transforming-growth-factor-beta and Immunoglobulin-G4-Related-Disease

Immunoglobulin G4-related disease (IgG4-RD) is a systemic immunological disorder characterized by fibro-inflammatory conditions; however, the pathobiology of IgG4-RD has not been fully identified.. This study aimed to analyze systemic differences of innate and adaptive immune cells from healthy controls and patients with IgG4-RD.. Healthy controls (n = 9) and IgG4-RD patients (n = 7) were recruited with informed consent. Peripheral blood was collected from healthy controls and IgG4-RD patients, and three blood samples from IgG4-RD patients were re-collected two months after the last rituximab (RTX) treatment. The various immune cells and cytokine productions were measured by flow cytometry.. Blood CD14+ monocytes and steady-state follicular helper T cells were increased in patients with IgG4-RD. However, there were no changes in other immune cell populations, including B cells, CD4 T cells, CD8 T cells, and innate lymphoid cells. Also, the TGF-β-producing CD14+ monocytes were significantly augmented in patients with IgG4-RD. Two months after RTX treatment, total B cells (CD19+) were depleted; however, the expressions of TGF-β from CD14+ monocytes remained unchanged.. These findings showed that IgG4-RD is related to the increment of CD14+ monocytes. Besides, controlling increased TGF-β-producing CD14+ monocytes with RTX treatment might be a conducive way to regulate IgG4-RD.

Topics: Adaptive Immunity; Aged; Cell Separation; Cells, Cultured; Female; Flow Cytometry; Germinal Center; Humans; Immunity, Innate; Immunoglobulin G4-Related Disease; Leukocytes, Mononuclear; Lipopolysaccharide Receptors; Male; Middle Aged; Monocytes; T-Lymphocytes, Helper-Inducer; Transforming Growth Factor beta