transforming-growth-factor-beta and Hypopharyngeal-Neoplasms

Transforming growth factor beta1 (TGFbeta1) is a negative growth regulator in keratinocytes, and in vitro studies lead to the concept that loss of TGFbeta1 responsiveness is a critical step in epithelial carcinogenesis.. To investigate the prognostic relevance of TGFbeta1 expression in head and neck squamous cell carcinoma (HNSCC).. TGFbeta1 distribution was determined by immunohistochemistry in oral cavity/oropharynx (n = 79), larynx (n = 36) and hypopharynx (n = 25) tumors and in matched normal adjacent mucosa. TGFbeta-type I and II receptors were determined in 20 cases of differentiated oral cavity/hypopharynx tumors. Cases were considered positive if displaying reactivity in >10% of the cells.. TGFbeta1-positive expression was found in 47.2% of larynx, 36.7% of oral cavity/oropharynx and in 24% of the hypopharynx tumors. Reactivity in >60% of the cells was displayed only by 11.4% of HNSCC. All normal controls were positive. TGFbeta1-positive expression did not correlate with clinico pathological parameters. An association with differentiation was verified only in oral cavity/oropharynx tumors (P

Topics: Activin Receptors, Type I; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Carcinoma, Squamous Cell; Case-Control Studies; Female; Gene Expression Regulation, Neoplastic; Head and Neck Neoplasms; Humans; Hypopharyngeal Neoplasms; Immunohistochemistry; Laryngeal Neoplasms; Male; Middle Aged; Mouth Neoplasms; Oropharyngeal Neoplasms; Prognosis; Protein Serine-Threonine Kinases; Receptor, Transforming Growth Factor-beta Type I; Receptor, Transforming Growth Factor-beta Type II; Receptors, Transforming Growth Factor beta; Survival Rate; Transforming Growth Factor beta; Transforming Growth Factor beta1

To detect the mRNA expression of transforming growth factor-betal (TGF-beta1) in laryngeal and hypopharyngeal carcinoma, and to explore the pathogenesis of the two types neoplasms.. We examined the expression of TGF-beta1 mRNA in the tissue of human laryngeal and hypopharyngeal carcinoma from patients. The TGF-beta1 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).. The TGF-beta1 mRNA expression was significantly stronger in 17 cases of 20 laryngeal and hypopharyngeal carcinoma.. The result suggested that the expression level of TGF-beta1 in laryngeal and hypopharyngeal carcinoma may inhibit the human immunity and enhance the growth and metastasis of the laryngeal and hypopharyngeal carcinoma.

Topics: Aged; Female; Humans; Hypopharyngeal Neoplasms; Laryngeal Neoplasms; Male; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Transforming Growth Factor beta; Transforming Growth Factor beta1