transforming-growth-factor-beta and Dermatofibrosarcoma

Dermatofibrosarcoma protuberans (DFSP) is a malignant tumor originating in the dermis. Although it is locally aggressive and recurs unless completely excised, it only rarely metastasizes. In the present study, we established 4 cultured DFSP cell strains, which were almost identical to normal skin fibroblasts when observed under a phase-contrast-microscope, and we observed their responses to various growth factors. DFSP cells showed significantly greater response to platelet-derived growth factor BB(PDGF BB) and transforming growth factor beta 1(TGF beta 1) than normal fibroblasts. We also determined upregulation of PDGF beta receptors in DFSP cells by both 125I PDGF-BB binding assay and immunoblotting analysis. These findings suggest that the interaction between the PDGF-B chain and the overexpression of PDGF beta receptors might play a role in the development of DFSP tumors.

Topics: Cell Division; Dermatofibrosarcoma; Growth Substances; Humans; Immunoglobulin G; Platelet-Derived Growth Factor; Receptors, Platelet-Derived Growth Factor; Skin Neoplasms; Transforming Growth Factor beta; Tumor Cells, Cultured; Up-Regulation