transforming-growth-factor-beta and Chondromatosis--Synovial

Synovial chondromatosis is characterized by the formation of osteocartilaginous nodules (free bodies) under the surface of the synovial membrane in joints. Free bodies and synovium isolated from synovial chondromatosis patients expressed high levels of BMP-2 and BMP-4 mRNAs. BMP-2 stimulated the expression of Sox9, Col2a1, and Aggrecan mRNAs in free-body and synovial cells and that of Runx2, Col1a1, and Osteocalcin mRNAs in the synovial [corrected] cells only. BMP-2 increased the number of alcian blue-positive colonies in the free-body cell culture but not in the synovial cell culture. Noggin suppressed the expression of Sox9, Col2a1, Aggrecan, and Runx2 mRNAs in both the free-body and synovial cells. Further, it inhibited Osteocalcin expression in the synovial cells. These results suggest that BMPs are involved in the pathobiology of cartilaginous and osteogenic metaplasia observed in synovial chondromatosis.

Topics: Aggrecans; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Carrier Proteins; Cartilage, Articular; Cells, Cultured; Chondromatosis, Synovial; Collagen Type II; Core Binding Factor Alpha 1 Subunit; Humans; Osteocalcin; Recombinant Proteins; SOX9 Transcription Factor; Transforming Growth Factor beta

A histopathological study of 30 cases of synovial osteochondromatosis found that the process followed a temporal sequence characterised by three phases: (I) active intrasynovial disease only; (II) transitional lesions with both active intrasynovial proliferation and free loose bodies; and (III) many free osteochondral bodies with no demonstrable intrasynovial disease [J. Bone Joint Surg. 59 (1977) 792]. We present five cases of synovial chondromatosis of the temporpmandibular joint (TMJ) which we studied by immunohistochemical methods of for transforming growth factor beta (TGFbeta) and tenascin.

Topics: Adult; Chondromatosis, Synovial; Female; Humans; Immunohistochemistry; Joint Loose Bodies; Temporomandibular Joint Disorders; Tenascin; Transforming Growth Factor beta

Synovial chondromatosis (SC) is an uncommon, benign condition of unknown etiology. A case of SC of the temporomandibular joint (TMJ) with the immunohistochemical findings of transforming growth factor-beta (TGF) and tenascin (TN) is reported. The roles of TGF and TN in SC of TMJ are discussed.

Topics: Adult; Arthroscopy; Cartilage, Articular; Chondromatosis, Synovial; Collagen; Coloring Agents; Endoscopy; Extracellular Matrix; Female; Humans; Hyperplasia; Immunoenzyme Techniques; Joint Loose Bodies; Synovial Membrane; Temporomandibular Joint Disorders; Tenascin; Transforming Growth Factor beta