transforming-growth-factor-alpha and Paranasal-Sinus-Neoplasms

To measure HPV status, epidermal growth factor receptor (EGFR) and transforming growth factor-alpha (TGF-alpha) expression and Ki-67 index in exophytic papilloma (EP), inverted papilloma (IP) with dysplasia, IP with carcinoma and invasive squamous cell carcinoma (SCC).. Forty-four patients with sinonasal papilloma and invasive SCC were selected. The nasal tissues were stained with monoclonal antibodies to EGFR, TGF-alpha and Ki-67. The results were analysed using quantitative immunohistochemical analysis. In situ hybridization studies for HPV DNA for 6/11, 16/18 and 31/33 were also performed on the tissue.. Significant increase of EGFR and TGF-alpha was observed in IP with severe dysplasia, IP with carcinoma and invasive SCC compared to IP with mild dysplasia and control nasal mucosa. And a serial upreguration in terms of Ki-67 index in IP with dysplasia was observed. Among IP, HPV 6/11-positive was present in 42% tumour and HPV 16/18-positive was present in 31% of tumours. Among HPV 6/11 and 16/18-positive IP, significant increase of EGFR and Ki-67 index were observed.. Pre-cancerous lesions of IP exhibited elevated levels of EGFR and TGF-alpha and these expression may be associated with early events in IP carcinogenesis. HPV infection may be an early event in a multistep process of malignant formation of IP.

Topics: Antibodies, Monoclonal; Biomarkers, Tumor; Carcinoma; Carcinoma, Squamous Cell; Cell Transformation, Neoplastic; ErbB Receptors; Female; Humans; In Situ Hybridization; Ki-67 Antigen; Male; Middle Aged; Nasal Mucosa; Neoplasm Invasiveness; Nose Neoplasms; Papilloma; Papilloma, Inverted; Papillomaviridae; Paranasal Sinus Neoplasms; Precancerous Conditions; Transforming Growth Factor alpha