tilianin has been researched along with Brain-Ischemia* in 1 studies
1 other study(ies) available for tilianin and Brain-Ischemia
| Article | Year |
|---|---|
Tilianin mediates neuroprotection against ischemic injury by attenuating CaMKII-dependent mitochondrion-mediated apoptosis and MAPK/NF-κB signaling.
Tilianin, a naturally occurring flavonoid glycoside, possesses versatile biological activities including antioxidant, anti-inflammatory, energy collecting and anti-hypoxic effects. Little is known about the mechanisms underlying the effect of tilianin against ischemic injury in neuronal cells. We aimed to determine the potential targets and mechanisms of tilianin treatment behind the crosstalk pathways induced by oxygen-glucose deprivation (OGD).. We used an in silico docking model for interaction mode analysis and in vitro models for mechanistic exploration and target verification. Protein changes were measured using cellular immunofluorescence and ELISA techniques.. Our study indicated that attenuation of CaMKII-linked signaling mediated through mitochondria and p38/JNK/NF-κB inflammatory pathways is a key mechanism by which tilianin exerts its neuroprotective effects against cerebral ischemia. Topics: Apoptosis; Brain Ischemia; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Cell Line, Tumor; Cell Survival; Enzyme-Linked Immunosorbent Assay; Flavonoids; Glucose; Glycosides; Humans; MAP Kinase Signaling System; Mitochondria; Molecular Docking Simulation; Neuroprotective Agents; NF-kappa B; Oxygen; p38 Mitogen-Activated Protein Kinases | 2019 |