thyronines and Starvation

The influence of an inhibitor of iodothyronines' extrathyroidal conversion on T4, T3 and rT3 deiodination by adult pig pituitary and cerebral cortical homogenates has been investigated. The homogenates were incubated with T4, T3 and rT3 in the presence of 5 mM dithiothreitol and evaporated diethyl ether extracts of sera obtained from fed and starved (1-14 days) rabbits. The extracts had no influence either on T4 to T3 or on T4 to rT3 conversion in cerebral cortex. Deiodination of rT3 to 3,3'-T2 in that tissue was significantly inhibited only by the extracts of sera obtained from 4 days starved rabbits. Inner-ring deiodination of both rT3 and T3 was not changed by the extracts got from short-term (1-4 days) fasted animals but was significantly reduced by the extracts from long-term (7-14 days) food-deprived subjects. Pituitary conversion of T4 to T3 was diminished by 35% in the presence of sera extracts gained from 1-9 days fasted rabbits and by about 50% on day 14 of fasting, but only the latter change was statistically significant. Short-term fasting inhibited T4 to rT3 conversion on days 2 and 4. Both deiodinations of rT3 and 5-deiodination of T3 were affected by extracts of sera collected during long-term fasting.

Topics: Animals; Cerebral Cortex; Diiodothyronines; In Vitro Techniques; Iodide Peroxidase; Pituitary Gland, Anterior; Rabbits; Starvation; Swine; Thyronines; Thyroxine; Tissue Extracts; Triiodothyronine; Triiodothyronine, Reverse

Characteristics of 3'-monoiodothyronine (3'-T1) degradation were examined in vitro in rat tissue homogenates. In rat liver homogenates, 3'-T1 degradation was optimal at pH 7.4, and was dependent upon time, temperature, and tissue concentration. The Michaeli's constant (Km) = 0.84 mumol/L. 3'-T1 degradation was enhanced by dithiothreitol and inhibited by propylthiouracil, sodium ipodate, ANS, and sodium azide but not by methimazole. Animals that fasted for three days had significant reductions in both hepatic T4 to T3 conversion (199 +/- 12 v 116 +/- 12 pg T3 generated/mg protein; P less than 0.001) and 3'-T1 degradation (588 +/- 31 v 148 +/- 53 pg 3'-T1 degraded/mg protein; P less than 0.001). To document that 3'-T1 degradation was occurring by deiodination, both liver and kidney homogenates were incubated with 125I-3'-T1 (approximately 3 microCi; 13.1 nmol/L). The reaction products were separated on a reverse-phase high pressure liquid chromatography (HPLC) column. In both tissues an iodide peak was generated, and no other radiolabeled peaks appeared except for 125I-3'-T1. These data suggest that 3'-T1 is metabolized by phenolic-ring monodeiodination and is enzymic in nature.

Topics: Animals; Chromatography, High Pressure Liquid; Hydrogen-Ion Concentration; In Vitro Techniques; Iodide Peroxidase; Iodine; Liver; Male; Peroxidases; Radioimmunoassay; Rats; Rats, Inbred Strains; Starvation; Thyroid Hormones; Thyronines

Topics: Animals; Diiodothyronines; Iodide Peroxidase; Kinetics; Liver; Male; Peroxidases; Radioimmunoassay; Rats; Rats, Inbred Strains; Starvation; Subcellular Fractions; Thyronines

Topics: Animals; Diiodothyronines; Dithiothreitol; Edetic Acid; Hyperthyroidism; Hypothyroidism; Iodide Peroxidase; Kinetics; Liver; Male; NADP; Peroxidases; Propylthiouracil; Rats; Starvation; Thyroidectomy; Thyronines; Triiodothyronine; Triiodothyronine, Reverse

Topics: Animals; Bile; Chromatography, High Pressure Liquid; Diiodothyronines; Male; Rats; Starvation; Thyronines; Thyroxine; Triiodothyronine; Triiodothyronine, Reverse

In order to compare, in vitro, the TSH suppressive effects of iodothyronines, rat pituitary quarters were first preincubated with T4, T3, rT3, or 3,3'-diiodothyronine (T2) in Gey and Gey buffer containing 1% bovine serum albumin for 2 h at 37 C and then incubated at 37 C for 1 h with the iodothyronine under study and TRH. TSH released into the medium during incubation was compared to that released by control pituitary fragments, which were not exposed to iodothyronines. All four iodothyronines (T3, T4, rT3, and T2) were able to significantly inhibit the TRH-induced release of TSH from pituitary fragments in a dose range of 0.015-2.2 microgram/ml. However, much larger doses of sodium iodide (1.25 mg/ml) and diiodotyrosine (10 and 30 microgram/ml) had no significant effect on the release of TSH. Among T3, rT3, and T4, T3 was the most potent and rT3 was the least potent. The relative potency of T3:T4:rT3 appeared to be approximately 100:12:1 when estimated from the lowest doses that caused significant inhibition of TRH-induced release of TSH, and approximately 100:6:0.5 when estimated from the doses that caused 50% inhibition of TSH release; the TSH inhibiting potency of T2 was similar to that of rT3. The activity of T4 could not be explained entirely on the basis of contamination of T4 with T3 or by in vitro conversion of T4 to T3. Similarly, the available data suggested that rT3 and T2 possess some, albeit modest, intrinsic TSH-Suppressive activity. TSH-inhibiting activities of T3, T4, and rT3 were also studied using pituitary fragments from starved and iodine-deficient rats. There was no evidence of a change in the sensitivity of the thyrotroph to either T3 or T4 in starvation. Similarly, comparison of the responses to several doses of rT3 did not indicate any significant abnormality in the sensitivity of the thyrotroph to rT3 in starvation or iodine deficiency. However, comparison of the TSH-suppressive effects of T4 in the iodine-deficient and normal rat indicated a significant increase in the sensitivity of the thyrotroph to T4 in iodine deficiency. A similar trend was also evident in the effect of T3 in iodine deficiency, but it fell short of statistical significance.

Topics: Animals; Diiodothyronines; In Vitro Techniques; Iodine; Male; Pituitary Gland, Anterior; Rats; Starvation; Thyronines; Thyrotropin; Thyrotropin-Releasing Hormone; Thyroxine; Triiodothyronine; Triiodothyronine, Reverse