thyronines and Acute-Disease

Mild acute pancreatitis (MAP) is a common acute abdominal disease, and exhibits rising incidence in recent decades. As an important component of systemic biology, metabonomics is a new discipline developed following genomics and proteomics. In this study, the objective was to analyze the serum metabonomics of patients with MAP, aiming to screen metabolic markers with potential diagnostic values.. An analysis platform with ultra performance liquid chromatography-high-resolution mass spectrometry was used to screen the difference metabolites related to MAP diagnosis and disease course monitoring.. A total of 432 endogenous metabolites were screened out from 122 serum samples, and 49 difference metabolites were verified, among which 12 difference metabolites were identified by nonparametric test. After material identification, eight metabolites exhibited reliable results, and their levels in MAP serum were higher than those in healthy serum. Four metabolites exhibited gradual downward trend with treatment process going on, and the differences were statistically significant (P < 0.05).. Metabonomic analysis has revealed eight metabolites with potential diagnostic values toward MAP, among which four metabolites can be used to monitor the disease course.

Topics: Acute Disease; Adult; Aged; Amylases; Chromatography, Liquid; Decanoates; Female; Glycocholic Acid; Humans; Lipase; Magnetic Resonance Imaging; Male; Mass Spectrometry; Metabolomics; Middle Aged; Pancreatitis; Principal Component Analysis; ROC Curve; Sphingosine; Support Vector Machine; Thyronines

Topics: Acute Disease; Diiodothyronines; Humans; Hyperthyroidism; Hypothyroidism; Liver Cirrhosis; Myocardial Infarction; Thyronines; Uremia

In order to study the alterations in thyroid hormone economy that accompany an acute bacterial infection, rhesus monkeys were inoculated i.v. with a virulent Diplococcus pneumoniae culture containing approximately 10(8) organisms per dose. This was found to produce a well-defined febrile illness followed in most instances by spontaneous recovery, thereby permitting sequential observations to be made during progression from the healthy state through acute infection into convalescence. During the acute febrile period of the infection, the clearance of both exogenously labeled L-thyroxine (T(4)) and 3,3',5-triiodo-L-thyronine (T(3)) from their peripheral pools was accelerated. This alteration was often evident by 8 hr after inoculation with the virulent culture and could not be ascribed to a decrease in extracellular binding. Despite the accelerated hormonal clearance, the concentrations of both endogenously labeled thyroid hormone and stable T(4) in the sera of the surviving monkeys remained essentially unchanged or increased, indicating that hormonal secretion must have increased during this period. During the convalescent period, hormonal clearance was similar to preinfection control values. Leukocytes isolated from blood obtained 6 hr after inoculation with the virulent culture displayed enhanced T(4)-deiodinative activity.

Topics: Acute Disease; Animals; Autoradiography; Blood Proteins; Electrophoresis; Haplorhini; Iodine; Iodine Isotopes; Leukocytes; Pneumococcal Infections; Protein Binding; Streptococcus pneumoniae; Thyronines; Thyroxine