thymosin-beta(4) and HIV-Infections

thymosin-beta(4) has been researched along with HIV-Infections* in 1 studies

Other Studies

1 other study(ies) available for thymosin-beta(4) and HIV-Infections

Article	Year
Modulation of cellular gene expression of HIV type 1 infection as determined by subtractive hybridization cloning: downregulation of thymosin beta 4 in vitro and in vivo. AIDS research and human retroviruses, 1994, Volume: 10, Issue:11 Chronic infection with HIV-1 has profound effects on host cell growth and function. We used subtractive hybridization cloning to identify genes whose expression is modulated by HIV-1 infection in the T leukemia cell line CEM. The gene encoding thymosin beta 4, a ubiquitous polypeptide associated with hematopoietic differentiation, showed two- to threefold reduced transcription in HIV-1-infected CEM cells and other HIV-1-infected T cells and macrophages in vitro. Solid-phase radioimmunoassay revealed about a threefold decrease in the level of thymosin beta 4 protein in lysates of infected cells. Northern blot analysis of RNA samples from lymphocytes of five AIDS patients reveals an up to fivefold reduction in the level of thymosin beta 4 mRNA. These results indicate that HIV-1 infection may directly influence the expression of certain physiologically important proteins. Topics: Cell Line; Cloning, Molecular; DNA, Complementary; Down-Regulation; Gene Expression; HIV Infections; HIV-1; Humans; Nucleic Acid Hybridization; RNA, Messenger; T-Lymphocytes; Thymosin	1994

Article

Year

Modulation of cellular gene expression of HIV type 1 infection as determined by subtractive hybridization cloning: downregulation of thymosin beta 4 in vitro and in vivo.

AIDS research and human retroviruses, 1994, Volume: 10, Issue:11

Chronic infection with HIV-1 has profound effects on host cell growth and function. We used subtractive hybridization cloning to identify genes whose expression is modulated by HIV-1 infection in the T leukemia cell line CEM. The gene encoding thymosin beta 4, a ubiquitous polypeptide associated with hematopoietic differentiation, showed two- to threefold reduced transcription in HIV-1-infected CEM cells and other HIV-1-infected T cells and macrophages in vitro. Solid-phase radioimmunoassay revealed about a threefold decrease in the level of thymosin beta 4 protein in lysates of infected cells. Northern blot analysis of RNA samples from lymphocytes of five AIDS patients reveals an up to fivefold reduction in the level of thymosin beta 4 mRNA. These results indicate that HIV-1 infection may directly influence the expression of certain physiologically important proteins.

Topics: Cell Line; Cloning, Molecular; DNA, Complementary; Down-Regulation; Gene Expression; HIV Infections; HIV-1; Humans; Nucleic Acid Hybridization; RNA, Messenger; T-Lymphocytes; Thymosin

1994