thymalfasin and Cell-Transformation--Neoplastic

The effects of thymosin alpha1 (Talpha1) on mammary carcinogenesis was investigated in Fisher rats. Mammary carcinomas were observed 3 months after N-nitrosomethylurea (NMU) injection (10mg, i.p.) into Fisher rats. Daily administration of Talpha1 (10 microg, s.c.) reduced mammary carcinoma incidence and prolonged survival time. Animals treated with exogenous Talpha1 had a significantly greater blood white cell density than control Fisher rats. These results suggest that Talpha1 prevents mammary carcinoma incidence as a result of stimulation of the immune system.

Topics: Animals; Blood Chemical Analysis; Cell Transformation, Neoplastic; Female; Leukocyte Count; Mammary Neoplasms, Experimental; Methylnitrosourea; Rats; Rats, Inbred F344; Thymalfasin; Thymosin

The preparations of alpha 1-thymosin (T), alpha-tumor necrotic factor (TNF) and their based hybrid proteins: T-TNF, TNF-T, and T-TNF-T were studied by using a wide spectrum of immunobiological tests. In the L-929 cells, T-TNF preserved cytotoxicity unique to TNF; TNF-T preserved it 10 times less, and T-NTF-T was completely inactive. TNF-T inhibited the growth of Molt-4, Jm-9, Raji cells by 63 = 84%, and TNF suppressed only Raji cells by 50%. Hybrid proteins caused cell proliferation of the thymus, spleen, and lymph nodes; H-2K, CD4, and CD8 differently increased the expression of thymocytic antigens. The authors found the different effects of the drugs on phagocytosis, the production of antibody-forming cells, delayed reaction, and activation of natural killer cells. The protein T-TNF-T produced the most pronounced action.

Topics: Animals; Antibody Formation; Biomarkers, Tumor; CD4-CD8 Ratio; Cell Division; Cell Line, Transformed; Cell Transformation, Neoplastic; Humans; Lymph Nodes; Lymphocyte Activation; Macrophages; Mice; Phagocytosis; Spleen; Thymalfasin; Thymosin; Thymus Gland; Tumor Necrosis Factor-alpha