thromboxane-b2 and Bronchiolitis--Viral

The effect of respiratory syncytial virus (RSV) antibody complexes on the metabolism of human neutrophils was determined by examining the generation of luminol-dependent chemiluminescence, superoxide, and thromboxane B2. Incubation of neutrophils with RSV antibody complexes resulted in a significant increase in the production of chemiluminescence. The increase in chemiluminescence appeared to be due to (1) active phagocytosis of RSV antibody complexes as evidenced by 70% inhibition with cytochalasin B (P less than 0.001) or (2) increased superoxide production as evidenced by 40% inhibition with superoxide dismutase (P less than 0.001). The generation of superoxide was confirmed by specific analysis in a superoxide dismutase-inhibitable ferricytochrome c reduction assay. Of particular importance was the observation that RSV antibody complexes induced the release of significant quantities of thromboxane B2 from neutrophils as determined by radioimmunoassay. Oxygen radicals and/or products of arachidonic acid metabolism may, in part, mediate the pathogenesis of RSV infection through direct tissue damage and bronchoconstriction.

Topics: Adult; Antibodies, Viral; Arachidonic Acid; Arachidonic Acids; Bronchiolitis, Viral; Cytochalasin B; Humans; Luminescent Measurements; Neutrophils; Respiratory Syncytial Viruses; Respirovirus Infections; Superoxides; Thromboxane B2