thromboxane-b2 and Arteriosclerosis-Obliterans

In order to identify local changes in prostanoids in arteriosclerosis obliterans (ASO), plasma levels of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1 alpha(6-Keto-PGF1 alpha) in cubital venous blood (CV blood), in femoral arterial blood (FA blood) and in femoral venous blood (FV blood) of 27 patients with ASO of lower limbs and of 10 healthy controls were measured by RIA technique. The following results were obtained. 1) In the ASO patients, levels of TXB2 and the ratio between TXB2 and 6-Keto-PGF1 alpha (TXB2/6-Keto-PGF1 alpha) in FV blood of ischemic legs were elevated in comparison to those in CV blood or FA blood of the same group or those in FV blood of the controls. 2) This change was more clearly observed in the femoro-popliteal group, but in the aorto-iliac+ combined group, they were elevated not only in FV blood but also in FA blood. 3) No significant difference was observed between the Fontaine I + II group and the Fontaine III + IV group in these local changes in prostanoids. 4) The severer the angiographic manifestation between the abdominal aorta and the common femoral artery, the higher the plasma TXB2 levels in FA blood. Also, the poorer the peripheral run-off, the higher the plasma TXB2 production in the legs. These results suggest that the imbalance between TXA2 and PGI2 is obvious in ischemic legs of ASO patients, TXA2 is elevated downstream from damaged vessels, and the local plasma TXB2 levels reflect the severity of the vascular damage in ASO patients.

Topics: 6-Ketoprostaglandin F1 alpha; Adult; Aged; Arteriosclerosis Obliterans; Female; Humans; Leg; Male; Middle Aged; Thromboxane B2

Erythrocytes deformability, plasma cAMP, 6-keto-PGF1 alpha, TXB2 levels and fibrinolytic activity in patients suffering from arteriosclerosis obliterans before and after Trental were studied. Trental improved the deformability of red cells and resulted in an enhancement of diminished cAMP level in plasma and increased the fibrinolytic activity. The improvement of deformability observed after Trental could depend upon the influence of a higher cAMP level in plasma on the erythrocyte membrane. The increase of cAMP level seems to be the result of the inhibiting effect of Trental on phosphodiesterase and of higher fibrinolytic activity.

Topics: 6-Ketoprostaglandin F1 alpha; Aged; Arteriosclerosis Obliterans; Cyclic AMP; Erythrocyte Deformability; Female; Fibrinolysis; Humans; Male; Middle Aged; Pentoxifylline; Rheology; Theobromine; Thromboxane B2

Topics: Aged; Arteriosclerosis Obliterans; beta-Thromboglobulin; Blood Platelets; Double-Blind Method; Drug Synergism; Epoprostenol; Female; Humans; Male; Middle Aged; Platelet Aggregation; Platelet Factor 4; Thiophenes; Thromboxane B2; Ticlopidine

Current concepts of atherogenesis based on animal and human investigations indicate prostaglandins as a key factor in atherosclerotic lesions. The plasma profiles of thromboxane B2 (TXB2), 6-keto-PGF1 alpha, PGE2, PGF2 alpha, and PGA1 were investigated by means of a sensitive radioimmunoassay technique in 40 patients with arteriosclerosis obliterans and in 30 healthy control subjects. Abnormally high levels of TXB2 and PGE2 (222.97 +/- 320.86 pg/ml, mean +/- SD, vs 20 +/- 2.1 and 352.66 +/- 235.54 vs 24.4 +/- 3, p less than 0.01) were detected in arteriosclerosis obliterans patients. The ratio between TXB2 and 6-keto-PGF1 alpha was increased from 1.2 in control subjects to 6.0 in patients. In arteriosclerosis obliterans TXB2 increased in relation to clinical manifestations and to the extension of the vascular damage. In addition, TXB2 was positively related to serum triglyceride content (r = 0.562, p less than 0.05) and inversely related to platelet count (r = 0.727, p less than 0.001). The marked imbalance between the stable metabolites of thromboxane and prostacyclin in arteriosclerosis obliterans patients provides biologic evidence which fits well with the thrombogenic theory of atherosclerosis. These results further support the theory that prostaglandins may be heavily involved in atherosclerosis.

Topics: 6-Ketoprostaglandin F1 alpha; Aged; Aortography; Arteriosclerosis Obliterans; Blood Pressure; Dinoprost; Dinoprostone; Humans; Male; Middle Aged; Prostaglandins; Prostaglandins A; Prostaglandins E; Prostaglandins F; Risk; Thromboxane B2; Thromboxanes; Triglycerides

The platelet MDA and plasma TXB2 formation was studied in normal subjects and in patients with obliterative arteriosclerosis of the lower limbs. The increase in platelet MDA and plasma TXB2 formation could be found in these patients. It is concluded that these factors as well as the decrease in plasma cAMP level previously reported may play decisive role in the pathogenesis of occlusive arterial disease.

Topics: Adult; Aged; Arteriosclerosis Obliterans; Blood Platelets; Cyclic AMP; Female; Humans; Leg; Male; Malonates; Malondialdehyde; Middle Aged; Thromboxane B2; Thromboxanes

In our ongoing studies of the interrelationship between platelets and the vascular wall we found that an accurate estimate could be made of the clinical condition and more effective therapy prescribed when we monitored alterations in the plasma levels of TXB2. For this purpose we devised a radioimmunoassay with I125-TXB2-Tyramide. Patients with ischemic heart disorders, cerebral apoplexy, diabetes mellitus, Buerger's disease, Takayasu disease etc., all had statistically high levels of TXB2 as compared with healthy controls. In particular, patients with myocardial infarction, cerebral thrombosis and/or hemorrhage all revealed increases in levels of TXB2 and these levels increased in parallel with a worsening of the clinical condition, and there was always a re-increase in TXB2 level before a recurrence of an attack. As plasma TXB2 levels clearly reflect thrombogenic disorders, the assessment of these levels on a routine basis, enables a more accurate diagnosis, an indication of possible recurrences, and more effective chemotherapy and rehabilitation.

Topics: Adolescent; Adult; Arteriosclerosis; Arteriosclerosis Obliterans; Child; Chromatography, Gel; Circadian Rhythm; Coronary Disease; Diabetes Mellitus; Dinoprost; Female; Humans; Intracranial Embolism and Thrombosis; Male; Middle Aged; Prostaglandins F; Takayasu Arteritis; Thromboangiitis Obliterans; Thromboxane B2; Thromboxanes