thromboplastin and Wounds--Penetrating

Essentials Methods were developed to image the hemostatic response in mouse femoral arteries in real time. Penetrating injuries produced thrombi consisting primarily of platelets. Similar to arterioles, a core-shell architecture of platelet activation occurs in the femoral artery. Differences from arterioles included slower platelet activation and reduced thrombin dependence.. Background Intravital studies performed in the mouse microcirculation show that hemostatic thrombi formed after penetrating injuries develop a characteristic architecture in which a core of fully activated, densely packed platelets is overlaid with a shell of less activated platelets. Objective Large differences in hemodynamics and vessel wall biology distinguish arteries from arterioles. Here we asked whether these differences affect the hemostatic response and alter the impact of anticoagulants and antiplatelet agents. Methods Approaches previously developed for intravital imaging in the mouse microcirculation were adapted to the femoral artery, enabling real-time fluorescence imaging despite the markedly thicker vessel wall. Results Arterial thrombi initiated by penetrating injuries developed the core-and-shell architecture previously observed in the microcirculation. However, although platelet accumulation was greater in arterial thrombi, the kinetics of platelet activation were slower. Inhibiting platelet ADP P2Y

Topics: Adenosine Diphosphate; Animals; Anticoagulants; Arterioles; Blood Coagulation; Blood Platelets; Femoral Artery; Fibrin; Hemodynamics; Hemostasis; Intravital Microscopy; Mice; Mice, Inbred C57BL; Microcirculation; Platelet Activation; Platelet Aggregation Inhibitors; Signal Transduction; Thrombin; Thromboplastin; Thrombosis; Wounds, Penetrating