thromboplastin and Toxemia

Topics: Animals; Blood Coagulation; Cytokines; Disseminated Intravascular Coagulation; Endotoxins; Factor VIIa; Fibrinolysis; Humans; Thromboplastin; Toxemia

Topics: Afibrinogenemia; Blood Coagulation; Blood Coagulation Tests; Diagnosis, Differential; Disseminated Intravascular Coagulation; Fibrinolysis; Hemostasis; Humans; Models, Biological; Syndrome; Thromboplastin; Toxemia

The expression of surface tissue factor procoagulant activity and its shedding by blood monocytes can be induced by several stimuli. Few of these defined situations, other than the presence of bacteria and their toxins, are commonly present in the young human infant. In this study, measurements were made of the percentage of monocytes expressing surface tissue factor apoprotein (TFA) in blood taken from babies in the early weeks of life. Mononuclear cells were separated from blood in an environment free of detectable endotoxin. After exposure to a polyclonal rabbit antibody raised to purified brain TFA and subsequent exposure to a fluorescin-labeled murine anti-rabbit IgG, the cell fluorescent activity was analyzed by flow cytometry. The percentage of monocytes showing strong fluorescence was determined. In every instance when systemic bacterial infection was present, more than 60% of the monocytes examined showed fluorescence indicative of the presence of surface TFA. In a single case of fungal Candida septicemia, none of the monocytes was positive. More than 60% of cells were found to be positive in certain instances where infection was highly probable but not proven. Positive cells were found in three cases of isoimmune hemolytic disease of the newborn, as had been anticipated from previous studies, whereas less than 25% of monocytes derived from babies in the absence of discernible infection or isoimmune hemolytic disease expressed surface TFA (p less than 0.001). These findings provide insight into a possible mechanism of coagulation activation in sepsis and may prove to be a useful predictor of the presence of infection or endotoxemia in young infants.

Topics: Apoproteins; Bacteremia; Bacterial Infections; Endotoxins; Erythroblastosis, Fetal; Humans; Infant; Infant, Newborn; Monocytes; Thromboplastin; Toxemia

We believe that toxic events observed after thermal injury may be caused by the release of normally intracellular substances into the circulation. We define these substances as 'metabolic' factors. Analysis of extracts prepared from normal and burned mouse skin indicates that the burned skin extract contains increased clot-promoting (Thromboplastin-like) substances and, perhaps, less RNA than normal skin extracts. Injection of RNA or its breakdown products into the burned site significantly increases the acute mortality in burned mice. No increase in mortality is observed when these substances are injected into a non-burned site on burned mice. We suggest that 'Thromboplastin-like substances and RNA or RNA breakdown products may be some of the 'metabolic' factors involved in acute burn toxicity. Upon being released from their intracellular residence after thermal injury, their combined activity contributes to the acute mortality observed.

Topics: Animals; Burns; DNA; Female; Mice; Nucleic Acids; RNA; Skin; Thromboplastin; Toxemia

Topics: Acute Kidney Injury; Anemia, Hemolytic; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Transfusion; Coronary Disease; Female; Heart Arrest; Hemorrhagic Disorders; Humans; Hyaline Membrane Disease; Infant, Newborn; Ischemia; Kidney Transplantation; Male; Obstetric Labor Complications; Pregnancy; Shock, Septic; Shwartzman Phenomenon; Thrombocytopenia; Thromboembolism; Thromboplastin; Toxemia; Wounds and Injuries

Topics: Chorion; Dicumarol; Female; Humans; Pre-Eclampsia; Pregnancy; Thromboplastin; Toxemia