thromboplastin and Laryngeal-Neoplasms

This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma.. Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed.. Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues.. TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.

Topics: Adult; Aged; Biomarkers, Tumor; Female; Head and Neck Neoplasms; Humans; Laryngeal Neoplasms; Male; Middle Aged; Thromboplastin

The blood coagulation mechanism may support tumor progression by several mechanisms including promotion of cell proliferation and angiogenesis. Immunohistochemical procedures were applied to AMeX-fixed sections of twelve cases of squamous cell carcinoma of the larynx obtained at surgical resection to determine the presence and distribution of tissue factor (TF), tissue factor pathway inhibitor (TFPI), other coagulation factors, fibrinogen, and fibrin in situ. TF antigen was present in normal squamous epithelial cells and tumor cells, predominantly in immature tumor cells in the vicinity of the host-tumor interface. Tumor cells stained also for factors VII and X. Staining for TFPI antigen was demonstrated in the connective tissue stroma adjacent to the tumor, in microvascular endothelial cells, and in normal squamous epithelial cells. Fibrinogen and factor XIIIa were distributed throughout the tumor connective tissue stroma. Fibrin (thrombin-cleaved fibrinogen) was detected at the host-tumor interface and along the margins of tumor nodules. Tumor cells in carcinoma of the larynx express a functional, TF-initiated pathway of blood coagulation. Interpretation of these findings together with the results of clinical trials of inhibitors of TF-induced coagulation activation versus effects of inhibitors of TF expression suggest novel approaches to the experimental therapy of laryngeal carcinoma.

Topics: Blood Coagulation; Carcinoma; Humans; Laryngeal Neoplasms; Lipoproteins; Neovascularization, Pathologic; Thromboplastin

It has been established that the only source of tissue thromboplastin activity in homogenate of cells (HeLa, L-41, RAMT, Hep-2 and FL) grown in synthetic nutrient media are permolecular formations represented by fragments of cellular membranes Thromboplastic activity of nutrient media is also associated with these formations, as fragments of cellular membranes enter the media during cell cultivation. The removal of all permolecular formations from homogenate of cells, from the medium in which they are cultivated, allows determination of the topography of tissue coagulation factors.

Topics: Amnion; Animals; Callitrichinae; Carcinoma, Squamous Cell; Cattle; Cell Line; Culture Media; Female; HeLa Cells; Humans; In Vitro Techniques; Kidney; Laryngeal Neoplasms; Leukemia; Male; Thromboplastin