thromboplastin and Hematologic-Diseases

Topics: Acute Disease; Animals; Disseminated Intravascular Coagulation; Hematologic Diseases; Heparin; Humans; Leukemia; Rabbits; Thromboplastin

There is compelling evidence that blood-borne tissue factor that is predominantly found on circulating microparticles (MPs) plays an important role in both, cancer biology and organ and stem-cell transplantation (SCT). Therefore, we hypothesized that numbers of tissue factor bearing MPs might be associated with complications and outcome in allogeneic SCT (allo-SCT).. In a prospective study, we enumerated total, platelet, endothelial, and tissue factor bearing MPs in plasma samples obtained from up to 60 patients with hematologic diseases at different time-points during the course of allo-SCT by flow cytometry. Patient- and transplant-related risk factors were included in statistical analysis.. Mean follow-up time was 968 days (0-1981 days). Thirty-four (56.7%) patients died, 17 due to transplant-related mortality (28.3%). High numbers of tissue factor positive MPs more than 500/μL before conditioning were predictive for shorter overall survival (P=0.017, hazard ratio=4.5) in multivariate analysis. This was mainly caused by an increase in transplant-related mortality (P=0.010, hazard ratio=11.0) with cumulative incidences at 1 year of 68.8% compared with patients with lower values (20.1%; P=0.002).. Tissue factor bearing MPs might be useful biomarkers for risk stratification in allo-SCT patients and further studies should investigate their origin, functional properties, and optimal cut-off values.

Topics: Adolescent; Adult; Aged; Biomarkers; Blood Platelets; Cell-Derived Microparticles; Endothelium, Vascular; Female; Flow Cytometry; Graft vs Host Disease; Hematologic Diseases; Humans; Incidence; Male; Middle Aged; Mucositis; Predictive Value of Tests; Prospective Studies; Recurrence; Risk Factors; Stem Cell Transplantation; Thromboembolism; Thromboplastin; Transplantation, Homologous; Young Adult

After developing a blood disorder, Yale Nemerson became interested in hematology. This led to his lifelong study of thrombogenic tissue factor and to his contributions to developing the modern theory of blood coagulation. The two Classic papers reprinted here detail some of Nemerson's studies on coagulation factors IX and VII.

Topics: Animals; Blood Coagulation; Factor IX; Factor VII; Hematologic Diseases; Hematology; History, 20th Century; Humans; Thromboplastin

To investigate the clinical significance of determination of plasma tissue factor (TF) antigen, we have developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) for plasma TF, using two different monoclonal antibodies against TF apoprotein, 6B4 (catching antibody) and 5G9 (detecting antibody), and tetramethyl benzidine/H2O2 as substrates. Titration curves of recombinant human TF in buffer containing Triton X-100 were linear within the range from 50 to 2000 pg/ml. The total assay time was 3 h. Ultracentrifugation and immunoblot analysis indicated that human plasma and urine contained 50,000 g sedimentable and non-sedimentable forms of TF, both of which were detected by our ELISA method. Plasma and urine concentrations of TF in healthy subjects and patients with various diseases were measured by the ELISA method. In healthy subjects, plasma and urinary TF levels were found to be 149 +/- 72 pg/ml (n = 30) and 175 +/- 60 pg TF/urine creatinine mg (n = 95), respectively. TF was increased in plasma of patients with disseminated intravascular coagulation (DIC), thrombotic thrombocytopenic purpura, vasculitis associated with collagen diseases, diabetic microangiopathy and chronic renal failure receiving haemodialysis, but not in the plasma of endotoxaemic patients without DIC. The plasma TF/serum creatinine ratio did not show a positive correlation. Measurement of TF antigen in plasma may be useful for evaluating the endothelial damage and cell destruction in TF-containing tissues.

Topics: Adult; Aged; Blood Coagulation Disorders; Diabetes Mellitus; Disseminated Intravascular Coagulation; Enzyme-Linked Immunosorbent Assay; Female; Hematologic Diseases; Humans; Immunoblotting; Male; Middle Aged; Purpura, Thrombotic Thrombocytopenic; Reference Standards; Thromboplastin; Ultracentrifugation; Vasculitis

Topics: Animals; Anticoagulants; Blood Coagulation Disorders; Blood Vessels; Dogs; Fibrinolysis; Hematologic Diseases; Hemorrhage; Hemostasis; Humans; Postoperative Complications; Rabbits; Rats; Stress, Physiological; Stress, Psychological; Surgical Procedures, Operative; Thromboplastin; Transfusion Reaction

Topics: Anemia; Anticoagulants; Blood Cell Count; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Platelets; Blood Transfusion; Fibrinogen; Fibrinolysis; Hematologic Diseases; Hemorrhage; Humans; Infant, Newborn; Postoperative Complications; Preoperative Care; Prothrombin; Prothrombin Time; Thromboplastin

Topics: Abruptio Placentae; Adult; Animals; Anuria; Bilirubin; Blood Coagulation Disorders; Dogs; Erythrocytes; Female; Fibrin; Fibrinolysis; Hematologic Diseases; Hemolysis; Humans; Mice; Pregnancy; Rabbits; Rats; Shock, Hemorrhagic; Thrombin; Thromboplastin

Topics: Anemia; Anemia, Hemolytic; Anemia, Hypochromic; Anemia, Pernicious; Blood Coagulation; Blood Proteins; Chloramphenicol; Epidemiology; Epoetin Alfa; Erythropoietin; Haptoglobins; Hematologic Diseases; Hematology; Hemochromatosis; Humans; Iron-Dextran Complex; Leukemia; Polycythemia; Thromboplastin; Vitamin B 12

Topics: Blood Coagulation Tests; Hematologic Diseases; Hemorrhage; Humans; Leukemia; Thrombocytopenia; Thromboplastin

Topics: Blood Coagulation Tests; Blood Platelets; Hematologic Diseases; Hemorrhagic Disorders; Humans; Platelet Function Tests; Thromboplastin

Topics: Diet; Dietary Fats; Hematologic Diseases; Humans; Lipids; Nutrition Assessment; Nutritional Status; Thromboplastin

Topics: Blood Platelets; Hematologic Diseases; Hemorrhagic Disorders; Thromboplastin

Topics: Cardiovascular Diseases; Hematologic Diseases; Humans; Prothrombin Time; Research; Sclerosis; Thrombophilia; Thromboplastin

Topics: Clinical Laboratory Techniques; Hematologic Diseases; Hemorrhagic Disorders; Thromboplastin

Topics: Hematologic Diseases; Hemorrhagic Disorders; Thromboplastin

Topics: Diagnosis, Differential; Hematologic Diseases; Hemophilia A; Hemophilia B; Hemorrhagic Disorders; Medicine; Thromboplastin