thromboplastin and Arthritis

Topics: Arthritis; Blood Coagulation Factors; Collagen Diseases; Diabetes Complications; Endocrine Glands; Glucocorticoids; Humans; Myeloproliferative Disorders; Obesity; Sex Factors; Thromboembolism; Thrombophlebitis; Thromboplastin; Thyroid Diseases

Fibrin accumulation in the joint cavity is a common feature of chronic arthritides, such as rheumatoid arthritis (RA). Complex formation between tissue factor (TF) and factor VII (FVII) is the initial step in such a fibrin formation.. To assess the role of the TF/FVII complex in the pathogenesis of joint inflammation, 1) the levels of TF/FVII complex were measured in synovial fluid of RA patients; 2) the complex was injected to healthy mice intra-articularly.. Morphological analysis of the joints 4 days after TF/FVII injection revealed influx of CD4-Mac1+ mononuclear leukocytes into synovial tissue followed by cartilage and bone destruction. Inflammation induced by TF/FVII complex was more profound than that caused by each of the proteins separately, both with respect to frequency, severity and duration of arthritis. Interaction between macrophages and lymphocytes in sustaining joint inflammation was proved by the requirement of the combined lymphocyte/ monocyte depletion to abolish TF/FVII induced arthritis. Induction of monocyte attracting chemokines (MIP-1 alpha and RANTES) was shown to be one of the potential mechanisms for TF/FVII complex triggered inflammatory cell influx. Interestingly, TF/FVII complexes were detected in synovial fluid of 20/40 patients with RA.. Altogether these findings indicate that TF/FVII complexes, frequently found intra-articularly in joints of RA patients, may be an important component in both induction and progression of chronic destructive arthritis.

Topics: Animals; Arthritis; Chemokines; Chronic Disease; Factor VII; Humans; Immune System; Injections, Intra-Articular; Mice; Mice, Inbred BALB C; Mice, Inbred Strains; Mice, SCID; Spleen; Synovial Fluid; Thromboplastin

Tissue factor (TF) is the major intrinsic initiator of clotting. TF expression on monocytes has been associated with high titers of anticardiolipin antibodies (aCL) in patients with antiphospholipid syndrome (APS) with thrombosis. We investigated the influence of clinical factors on TF activity in blood from patients with systemic lupus erythematosus (SLE) and examined the relationship between aCL and TF.. In this cross sectional study, consecutive patients with SLE from one rheumatology clinic gave blood samples for measurement of TF activity, aCL, and Russell viper venom time. We assessed disease activity by measuring sedimentation rate, anti-dsDNA, and complement components C3 and C4, and measured clinical indices. Associations were investigated with the Wilcoxon rank-sum test and linear regression.. Sixty-nine patients contributed blood samples. The median age was 38 years, and 10 of the SLE patients had a history of thrombosis. Patients with active arthritis had TF activity 1.3 times that in patients without arthritis (p = 0.028). Users of nonsteroidal antiinflammatory drugs (NSAID) had TF activity significantly lower than nonusers (p = 0.010). Patients with previous thrombosis had TF activity significantly lower than patients without thrombosis (p < 0.001). Overall, aCL and TF activity were not associated when we adjusted for these clinical factors.. Arthritis, previous thrombosis, and use of NSAID significantly modified TF activity in patients with SLE. Unlike previous reports, we found no association between aCL titer and TF activity, which may be due to our adjusting for other important clinical factors.

Topics: Adolescent; Adult; Aged; Anti-Inflammatory Agents, Non-Steroidal; Antibodies, Anticardiolipin; Arthritis; Cross-Sectional Studies; Disease Progression; Female; Humans; Lupus Erythematosus, Systemic; Male; Middle Aged; Thromboplastin; Thrombosis

Topics: Animals; Arthritis; Blood Coagulation Disorders; Chlorambucil; Chromatography, Gel; Electrophoresis, Starch Gel; Factor VIII; gamma-Globulins; Humans; Immune Sera; Immune System Diseases; Immunoglobulin M; Male; Middle Aged; Prothrombin Time; Rabbits; Raynaud Disease; Thromboplastin

Topics: Adult; Aged; Arthritis; Blood Coagulation Disorders; Blood Coagulation Tests; Blood Platelets; Carcinoma; Factor VIII; Female; Fibrinolysin; Hepatitis; Humans; Liver Diseases; Male; Middle Aged; Necrosis; Rectal Neoplasms; Thrombin; Thromboplastin; Vaginal Neoplasms

Topics: Arthritis; Blood Coagulation Factors; Factor XI; Factor XII; Gout; Humans; In Vitro Techniques; Inflammation; Synovial Fluid; Thromboplastin

Topics: Anticoagulants; Arthritis; Arthritis, Rheumatoid; Hemorrhagic Disorders; Medical Records; Thromboplastin

Topics: Anticoagulants; Arthritis; Arthritis, Rheumatoid; Blood Coagulation; Hemorrhagic Disorders; Humans; Thromboplastin