thromboplastin and Arteriosclerosis-Obliterans

In systemic lupus erythematosus (SLE) patients, the prevalence of arteriosclerosis obliterans (ASO) is high despite a lack of common risk factors for ASO. The main objective of this study was to investigate a possible direct role of anti-phospholipid antibodies (aPLs), which are frequently detected in SLE patients, in the pathogenesis of ASO.. We examined tissue factor (TF) expression on the monocyte surface by flow cytometric analysis in 89 SLE patients with or without ASO and/or aPLs and studied the in vitro effect of purified IgG fractions from plasma of SLE patients or normal healthy volunteers (aPLs(+) IgG, n=8; aPLs(-) IgG, n=6; Normal IgG, n=6) on the expression of TF and production of TNF-α and IL-1β in healthy peripheral blood mononuclear cells (PBMCs) or isolated monocytes.. We confirmed that high expression of monocyte TF was strongly associated with the prevalence of ASO and the presence of aPLs. Treatments of PBMCs with aPLs(-) IgG or normal IgG did not significantly increase expression of TF, TNF-α, and IL-1β messenger RNA (mRNA) and the production of TNF-α and IL-1β. However, stimulation of PBMCs with aPLs(+) IgG caused significant increase in expression of TF, TNF-α, and IL-1β mRNA. Moreover, aPLs(+) IgG stimulated PBMCs and significantly enhanced the production of TNF-α and IL-1β.. These results suggest that IgG-aPLs cause persistently high TF expression and inflammatory cytokine production by interacting with peripheral blood monocytes and lymphocytes, which may be an important mechanism in the pathogenesis of ASO peculiar to SLE patients.

Topics: Adolescent; Adult; Aged; Antibodies, Antiphospholipid; Arteriosclerosis Obliterans; Child; Cytokines; Female; Gene Expression Regulation; Humans; Immunoglobulin G; Leukocytes, Mononuclear; Lupus Erythematosus, Systemic; Male; Middle Aged; Monocytes; Thromboplastin; Tumor Necrosis Factor-alpha; Young Adult

The wall of lover-limb arteries affected with severe atherosclerosis contains a Complex of substances with pro- and anticoagulative activities. The arterial wall, traumatized in endarterectomy by separation of the individual coats, releases procoagulative substances into blood circulation. The most conspicuous local manifestations of hypercoagulation and hypofibrinolysis appear on the day of surgery. The artery operated upon releases the thromboplastic factor for nine days; substances shortening the thrombin time (antiheparin substance, thrombin accelerator), for five days; and inhibitors of fibrinolysis, for four days after operation. A correlation was found between the regenerative process in the endarterectomized artery and the dynamics of the release of tissue factors influencing the haemocoagulation.

Topics: Animals; Arteriosclerosis Obliterans; Blood Coagulation; Catheterization; Dogs; Endarterectomy; Fibrin; Fibrinolysis; Humans; Iliac Artery; Perfusion; Postoperative Complications; Thrombin; Thromboplastin; Time Factors

Topics: Arteriosclerosis Obliterans; Aspergillus; Blood Coagulation; Blood Coagulation Tests; Electrocardiography; Fibrinogen; Fibrinolysis; Humans; Immunoelectrophoresis; In Vitro Techniques; Injections, Intravenous; Pelvis; Peptide Hydrolases; Retinal Artery; Thromboangiitis Obliterans; Thrombophlebitis; Thromboplastin; Urticaria

Topics: Adult; Aged; Antithrombins; Arteries; Arteriosclerosis Obliterans; Blood Coagulation; Blood Coagulation Tests; Femoral Artery; Fibrin; Fibrinolysis; Heparin Antagonists; Humans; Iliac Artery; Middle Aged; Plasminogen; Thromboplastin

Topics: Adult; Animals; Arteriosclerosis Obliterans; Blood Coagulation; Diabetes Mellitus; Diabetic Angiopathies; Heparin; Humans; Methods; Middle Aged; Rabbits; Smoking; Thromboplastin

Topics: Aged; Amputation, Surgical; Arteriosclerosis Obliterans; Blood Coagulation Disorders; Chronic Disease; Coumarins; Diabetes Mellitus; Follow-Up Studies; Humans; Hypercholesterolemia; Hypertension; Middle Aged; Myocardial Infarction; Thromboplastin

Topics: Arteriosclerosis Obliterans; Blood Coagulation; Blood Coagulation Tests; Cerebrovascular Disorders; Coronary Disease; Humans; Myocardial Infarction; Thrombelastography; Thromboplastin

Topics: Arteries; Arteriosclerosis; Arteriosclerosis Obliterans; Humans; Thromboplastin