thiourea and Staphylococcal-Infections

The increasing resistance of bacteria to commercially available antibiotics threatens patient safety in healthcare settings. Perturbation of ion homeostasis has emerged as a potential therapeutic strategy to fight against antibacterial resistance and other channelopathies. This study reports the development of 8-aminoquinoline (QN) derivatives and their transmembrane Zn

Topics: Animals; Anti-Bacterial Agents; Humans; Ionophores; Mammals; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Quinolines; Staphylococcal Infections; Thiourea; Zinc

Topics: Anti-Bacterial Agents; Biofilms; Humans; Microbial Sensitivity Tests; Plankton; Staphylococcal Infections; Staphylococcus aureus; Thiourea

To test the effect that the treatment with topical antioxidants may have on corneal infection, we have studied the effect of topically applied antioxidants, such as dimethylthiourea 0.5% (DMTU) and dismutase superoxide 0.2% (SOD), on infectious experimental keratitis caused by Staphylococcus aureus. We have quantified the results of the incubated corneas in ex vivo as well as in in vivo treated with antioxidants by using the luminol amplified chemiluminescence technique (LAC). The evaluation of corneal inflammation was performed calculating the average inflammatory index obtained from the clinical observation of the corneal secretion, corneal edema and ciliary injection. The evolution of the corneal infiltration was evaluated by means of computerized planymetry. The antioxidants used in this study demonstrated a significant reduction of the LAC values when compared with a control group both in the in vivo as well as in ex vivo studies. No significant differences in the clinical evaluation of the average inflammatory index were observed between the study and the control groups. However, a significant increase in the corneal infiltration was registered in the antioxidant treated group (p < 0.001) evaluated by computerized planymetry. Our results indicate that the use of antioxidants as antiinflammatory drugs may have a potential negative influence on the course of infectious keratitis.

Topics: Administration, Topical; Animals; Antioxidants; Disease Models, Animal; Eye Infections, Bacterial; Keratitis; Ophthalmic Solutions; Rabbits; Staphylococcal Infections; Superoxide Dismutase; Thiourea

The clinically used topical antibiotic ointments, gentamicin 0.1%, oxytetracycline 3% w/polymyxin 0.1% and chloramphenicol 2% and an experimental preparation, 10-undecen-1-yl thiopseudourea iodide (AHR-1911) were studied for anti-infective action applied externally on the skin of mice inoculated subcutaneously with S. aureus and E. coli. In both infections statistically significant difference was encountered between curative and "clinical" healing rate in the case of the less effective preparations and/or dosages. The method appears suitable to establish and to compare the in vivo activity of ointments. This is not directly related to that of the aqueous drug solutions. AHR-1911, a powerful inhibitor of nucleic acid and protein synthesis, showed a bimodal action, with maximal effectiveness at 0.12-0.25% concentration, due presumably to the anti-inflammatory effect of higher concentrations. Direct evidence for the absorption of the drug from the application site was obtained using 14C labeled AHR-1911.

Topics: Abscess; Administration, Topical; Animals; Drug Combinations; Drug Evaluation; Escherichia coli Infections; Gentamicins; Isothiuronium; Mice; Ointments; Oxytetracycline; Polymyxins; Skin Ulcer; Staphylococcal Infections; Thiourea

Topics: Amphotericin B; Animals; Anti-Bacterial Agents; Anti-Infective Agents; Bacterial Infections; Candida albicans; Candidiasis; Escherichia coli; Escherichia coli Infections; Mice; Neomycin; Nystatin; Penicillins; Polymyxins; Staphylococcal Infections; Streptomycin; Tetracycline; Thiourea; Undecylenic Acids

Topics: Animals; Aorta, Abdominal; Bacterial Infections; Constriction; Hypoxia; Lung; Macrophages; Male; Mice; Phagocytosis; Phosphorus Radioisotopes; Pneumonia; Pulmonary Alveoli; Pulmonary Edema; Rats; Staphylococcal Infections; Thiourea

Topics: Acetylcholine; Administration, Topical; Animals; Anti-Bacterial Agents; Anti-Infective Agents; Anti-Inflammatory Agents; Antifungal Agents; Arthritis; Arthrodermataceae; Bacteria; Blood Pressure; Burns; Candida; Candidiasis; Carrageenan; Depression, Chemical; Dextrans; Gastrointestinal Motility; Guinea Pigs; Hindlimb; Kaolin; Mice; Rabbits; Rats; Staphylococcal Infections; Thiourea

Topics: Bacteria; Escherichia coli; Guanidines; Klebsiella Infections; Mercaptoethylamines; Methods; Mycobacterium tuberculosis; Pseudomonas aeruginosa; Salmonella typhimurium; Shigella sonnei; Staphylococcal Infections; Staphylococcus; Streptococcus pneumoniae; Streptococcus pyogenes; Sulfides; Thiosulfates; Thiourea

Topics: Humans; Staphylococcal Infections; Streptococcal Infections; Sulfanilamide; Sulfanilamides; Sulfonamides; Thiourea