thiourea and Leptospirosis

thiourea has been researched along with Leptospirosis* in 1 studies

Other Studies

1 other study(ies) available for thiourea and Leptospirosis

Article	Year
Technical improvement to prevent DNA degradation of Leptospira spp. in pulsed field gel electrophoresis. Letters in applied microbiology, 2009, Volume: 49, Issue:2 Leptospirosis is a public health problem. Infection with pathogenic Leptospira occurs by exposure to many environments and is traditionally associated with occupational risk activities. Pulsed-field gel electrophoresis was used to investigate the epidemiological relatedness among Leptospira isolates. However, analysis by PFGE yielded inconclusive data as a result of extensive DNA degradation. This degradation can be significantly reduced by the inclusion of thiourea in the electrophoresis buffer, improving the analysis of DNA banding patterns. Topics: Animals; Bacterial Typing Techniques; Deoxyribonucleases; DNA Fingerprinting; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Humans; Leptospira; Leptospirosis; Thiourea	2009

Article

Year

Technical improvement to prevent DNA degradation of Leptospira spp. in pulsed field gel electrophoresis.

Letters in applied microbiology, 2009, Volume: 49, Issue:2

Leptospirosis is a public health problem. Infection with pathogenic Leptospira occurs by exposure to many environments and is traditionally associated with occupational risk activities. Pulsed-field gel electrophoresis was used to investigate the epidemiological relatedness among Leptospira isolates. However, analysis by PFGE yielded inconclusive data as a result of extensive DNA degradation. This degradation can be significantly reduced by the inclusion of thiourea in the electrophoresis buffer, improving the analysis of DNA banding patterns.

Topics: Animals; Bacterial Typing Techniques; Deoxyribonucleases; DNA Fingerprinting; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Humans; Leptospira; Leptospirosis; Thiourea

2009