thiourea and Hypertrophy

Physiopathological discrepancies exist between the most widely used models of pulmonary hypertension (PH), namely monocrotaline- and hypoxia-induced PH. The development of a new model could help in the understanding of underlying mechanisms. Repeated alpha-naphthylthiourea (ANTU) injections (5 mg/kg weekly, 3 wk) induced pulmonary vascular remodeling, which was associated with development of PH and right ventricular hypertrophy. ANTU followed by granulocyte colony-stimulating factor (G-CSF; 25 microgram. kg(-1). day(-1) subcutaneously, 3 days/wk) induced higher pulmonary arterial pressures and right ventricular hypertrophy than ANTU alone. Lidocaine, which inhibits neutrophil functions, inhibited PH exacerbation by G-CSF. Endothelial nitric oxide synthase expression, measured to assess ANTU-related endothelial toxicity, decreased significantly in ANTU-treated rats and fell even more sharply when G-CSF was given. This occurred despite a significant increase in vascular endothelial cell growth factor expression in lung and right ventricle in rats given ANTU alone and even more in rats given ANTU plus G-CSF. Repeated ANTU administration induces PH with vascular remodeling that can be further aggravated by the neutrophil activator G-CSF.

Topics: Animals; Blood Vessels; Blotting, Western; Body Weight; Chronic Disease; Drug Synergism; Granulocyte Colony-Stimulating Factor; Hypertension, Pulmonary; Hypertrophy; Lung; Male; Nitric Oxide Synthase; Nitric Oxide Synthase Type III; Organ Size; Rats; Rats, Sprague-Dawley; Thiourea; Vascular Endothelial Growth Factor A

Topics: Animals; Cell Count; Gastric Juice; Gastric Mucosa; Gastrins; Histamine H2 Antagonists; Hypertrophy; In Vitro Techniques; Metiamide; Pentagastrin; Rats; Receptors, Histamine H2; Sodium Chloride; Stimulation, Chemical; Thiourea; Time Factors

Topics: Animals; Connective Tissue; Cysts; Epithelial Cells; Epithelium; Fishes; Gonads; Hyperemia; Hyperplasia; Hypertrophy; Injections, Intraperitoneal; Iodine Isotopes; Pituitary Gland; Temperature; Thiourea; Thyroid Diseases; Thyroid Gland

Topics: Cystitis; Drug Combinations; Escherichia coli; Escherichia coli Infections; Humans; Hypertrophy; Male; Prostatic Hyperplasia; Sulfadiazine; Sulfonamides; Thiourea

Topics: Goiter; Hypertrophy; Thiourea; Thyroid Gland