thiouracil and Adenoma

Topics: Adenoma; Carbimazole; Child; Eye Manifestations; Female; gamma-Globulins; Graves Disease; Hot Temperature; Humans; Hyperkinesis; Hyperthyroidism; Iodides; Iodine Isotopes; Long-Acting Thyroid Stimulator; Pregnancy; Sweating; Thiouracil; Thyroid Function Tests; Thyroidectomy; Thyroxine

Topics: Adenoma; Animals; Dogs; Fluorenes; Humans; Iodine; Iodine Radioisotopes; Mice; Neoplasm Metastasis; Neoplasm Transplantation; Neoplasms, Experimental; Neoplasms, Radiation-Induced; Radiation Effects; Radiotherapy; Rats; Sheep; Thiouracil; Thiourea; Thyroid Gland; Thyroid Neoplasms; Thyroidectomy; Transplantation, Homologous

Topics: Adenoma; Alpha-Globulins; Antibody Formation; Antigens; Autoantibodies; Autoimmune Diseases; Beta-Globulins; Blood Proteins; Child; Humans; Hyperthyroidism; Hypothyroidism; Organ Specificity; Serum Globulins; Species Specificity; Thiouracil; Thyroid Function Tests; Thyroid Hormones; Thyroid Neoplasms; Thyroiditis; Thyroiditis, Autoimmune; Thyrotropin; Triiodothyronine

To investigate the cell cycle kinetics during the tumor promotion process induced by hypothyroidism in a rat model of thyroid follicular cell carcinogenesis, immunohistochemical analysis of cell cycle molecules and related signaling molecules was performed in conjunction with analysis of cell proliferation activity in an initiation-promotion model. Male F344 rats were injected with N-bis(2-hydroxypropyl)nitrosamine, and one week later treated with 6-propyl-2-thiouracil (PTU) at 12ppm in the drinking water for 4, 10 or 15 weeks. At each time point, proliferative lesions increased the expression of cyclin A, cyclin D, cyclin E and cyclin-dependent kinase (Cdk)-2, in association with the development of lesion stages from the early focal hyperplasia to the late carcinoma, while a subpopulation of proliferative lesions showed decreased numbers of both cell division cycle-2- and Ki-67-positive cells at week 15 compared with that at week 10, suggesting a reduced promoting effect of serum thyroid-stimulating hormone in the sensitive cellular population after long-term exposure to PTU. On the other hand, increased immunolocalization of phosphorylated and inactive glycogen synthase kinase (GSK)-3beta was observed in a subpopulation of proliferative lesions, in parallel with the cyclins and Cdk2. Nuclear immunoreactivity of phosphorylated and inactive retinoblastoma (Rb) protein was also increased in association with lesion development, with carcinomas showing increased cytoplasmic localization. The results suggest that proliferative lesions activate the cell cycle machinery following tumor promotion via a regulatory mechanism involving inactivation of GSK3beta and Rb protein, the latter signaling mechanism involving its aberrant nucleocytoplasmic transport for the acquisition of a malignant phenotype.

Topics: Adenocarcinoma, Follicular; Adenoma; Animals; Carcinogens; Cell Cycle Proteins; Cell Nucleus; Cell Transformation, Neoplastic; Cytoplasm; Disease Models, Animal; Glycogen Synthase Kinase 3; Glycogen Synthase Kinase 3 beta; Immunohistochemistry; Male; Nitrosamines; Protein Transport; Rats; Rats, Inbred F344; Retinoblastoma Protein; Signal Transduction; Thiouracil; Thyroid Neoplasms

Topics: Adenoma; Graves Disease; Humans; Hyperthyroidism; Iodine; Iodine Isotopes; Perchlorates; Thiouracil; Thyroglobulin; Thyroid Neoplasms; Thyroidectomy

Topics: Adenoma; Antithyroid Agents; Graves Disease; Humans; Hyperthyroidism; Imidazoles; Iodine; Perchlorates; Potassium; Radiography; Radionuclide Imaging; Sulfhydryl Compounds; Thiouracil; Thyroid Neoplasms; Thyroidectomy

Topics: Adenoma; Animals; Carcinogens; Carcinoma; Fluorenes; Methylthiouracil; Neoplasm Metastasis; Neoplasms, Experimental; Propylthiouracil; Rats; Thiouracil; Thyroid Neoplasms

Topics: Adenoma; Humans; Neoplasms; Pregnancy; Thiouracil; Thyroid Gland; Viscera

Topics: Adenoma; Adult; Humans; Hyperthyroidism; Neoplasms; Thiouracil; Thyroid Gland; Thyroid Neoplasms; Thyrotoxicosis