thiomuracin-a and Gram-Positive-Bacterial-Infections

Synthetic studies of the antimicrobial secondary metabolite thiomuracin A (1) were initiated to improve chemical stability and physicochemical properties. Functional group modifications of 1 included removing the C2-C7 side chain, derivatizing the C84 epoxide region, and altering the C44 hydroxyphenylalanine motif. The resulting derivatives simplified and stabilized the chemical structure and were evaluated for antibacterial activity relative to 1. The simplified structure and improved organic solubility of the derivatives facilitated isolation yields from fermentation broths and simplified the procedures involved for the process. These advancements increased material supply for continued medicinal chemistry optimization and culminated in the identification of 2, a structurally simplified and chemically stable analogue of 1 which retained potent antibiotic activity.

Topics: Animals; Anti-Bacterial Agents; Clostridioides difficile; Crystallography, X-Ray; Enterococcus; Escherichia coli; Escherichia coli Proteins; Female; Gram-Positive Bacterial Infections; Male; Mice; Microbial Sensitivity Tests; Models, Molecular; Molecular Structure; Peptide Elongation Factor Tu; Peptides, Cyclic; Protein Synthesis Inhibitors; Rats; Rats, Sprague-Dawley; Staphylococcus aureus; Streptococcus pyogenes; Structure-Activity Relationship; Thiazoles; Transcription, Genetic