thiobarbituric-acid and Burns

To determine xanthine oxidase and superoxide dismutase activities, lipid peroxidation, protein carbonylation, and total radical-trapping antioxidant parameter in survivors and nonsurvivors patients with severe burn injury.. Prospective, comparative observational study in an intensive care unit, burn division, in a trauma hospital.. Twenty-five consecutive patients who met the established criteria for severe burn injury (total burn surface area of more than 30%).. Plasma thiobarbituric acid reactive species and protein carbonyls levels were significantly higher in nonsurvivors than in survivors at 0 and 6 h. Elevated xanthine oxidase activity at 0 h was associated with adverse outcome after burn injury. In contrast, plasma superoxide dismutase activity and total radical-trapping antioxidant parameter did not differ significantly between nonsurvivors and survivors at any time point.. For the first time we demonstrate the value of oxidative parameters, namely thiobarbituric acid reactive species, protein carbonyls, and xanthine oxidase activity, in identifying burn patients with a poor prognosis. Whether these parameters are merely markers of clinical course, or whether they signal specific deleterious effects of oxidative stress during the burn injury remains to be elucidated.

Topics: Adult; Biomarkers; Blood Proteins; Burns; Critical Care; Female; Humans; Lipid Peroxidation; Male; Oxidation-Reduction; Prognosis; Prospective Studies; Superoxide Dismutase; Thiobarbiturates; Xanthine Oxidase

The role of lipid peroxidation in reduced red cell deformability has been examined after thermal injury of rats (full skin thickness over 15-20 per cent of total body surface). An increased concentration of erythrocyte malonyldialdehyde (MDA) and blood thiobarbituric acid (TBA) reactive product levels during the first 3 days after thermal trauma was established. There was a remarkable decrease in red cell superoxide dismutase (SOD) activity after burns and an increase in glucose-6-phosphate dehydrogenase (G-6-PD) on day 3 postburn. The deformability of erythrocytes was diminished significantly during the first 3 days after burns. There was a significant negative correlation between the deformability and the amount of MDA accumulated in erythrocytes (r = -0.92). Both the accumulation of MDA and the reduction of SOD activity in erythrocytes were suppressed by alpha-tocopherol treatment, which also prevented the decrease in erythrocyte deformability. These results suggest that the activation of the peroxidative process is a possible mechanism for the decreased deformability of erythrocytes during the early stage after thermal injury.

Topics: Analysis of Variance; Animals; Burns; Erythrocyte Deformability; Erythrocyte Membrane; Glucosephosphate Dehydrogenase; Lipid Peroxidation; Male; Malondialdehyde; Rats; Rats, Wistar; Superoxide Dismutase; Thiobarbiturates; Vitamin E

To elucidate the pathogenesis of acute gastric mucosal lesion (AGML), burn stress was loaded in rats with and without obstructive jaundice. The activation of gastric mucosal glycosidases (beta-N-acetyl-D-glucosaminidase (NAG), beta-glucuronidase (BG)), which were released into the cytoplasm as a results of instability of the lysosomal membrane, was studied biochemically, enzymatically and histochemically after burn stress with and without obstructive jaundice. The latent enzyme activity calculated by NAG or BG, which represented the stability of lysosomal membrane, was lowest at 2-3 hour after burn stress in both groups. In other words, the degree of activation of the glycosidases was highest at 2-3 hour after burn stress. The latent enzyme activity calculated by NAG decreased significantly (p < 0.05) at 1 hour after burn stress in obstructive jaundice group compared with the non-obstructive jaundice group. The staining of NAG before burn stress was observed in mucus neck cell and surface epithelial cell in granular shape and it was observed diffusely after burn stress, especially in obstructive jaundice group. The changes in staining of BG was similar to NAG. Before activation of glycosidases, the thiobarbituric acid reactants, which are considered products of lipid peroxidation, increased promptly at 30 min after burn stress in both groups. Ulcer index increased gradually after burn stress and the significance was found between in the obstructive jaundice group and in non-obstructive jaundice group at 3 hour after burn stress. It's concluded that obstructive jaundice accelerated the fragility of lysosomal membrane after burn stress and these change were considered to be the reasons of frequent occurrence of AGML.

Topics: Acetylglucosaminidase; Animals; Burns; Cholestasis; Gastric Mucosa; Glycoside Hydrolases; Male; Rats; Rats, Wistar; Stress, Physiological; Thiobarbiturates

Alterations in the erythrocyte rheology and the contents of activated free radical oxidation products (conjugated dienes, products of thiobarbituric acid and Schiff bases) in the acute phase of experimental thermic injury of the skin were studied. Erythrocyte flexibility reduction and erythrocyte aggregation increase correlated with elevated amounts of free radical oxidation products. Alpha-tocopherol avoided the accumulation of free radical oxidation products and improved both antioxidant defence and erythrocyte rheology. Thus we suppose that free radical oxidation products probably participate in the pathogenesis of erythrocyte rheology disturbances after thermic trauma.

Topics: Animals; Burns; Erythrocyte Aggregation; Erythrocyte Deformability; Filtration; Free Radicals; Hemoglobins; Kinetics; Lipid Peroxidation; Male; Oxidation-Reduction; Rats; Rats, Inbred Strains; Schiff Bases; Thiobarbiturates; Vitamin E

Topics: Animals; Burns; Gastric Mucosa; Lipid Peroxides; Male; Rats; Rats, Inbred Strains; Thiobarbiturates