thioacetamide and Sepsis

Dectin-1 is a C-type lectin receptor critical in anti-fungal immunity, but Dectin-1 has not been linked to regulation of sterile inflammation or oncogenesis. We found that Dectin-1 expression is upregulated in hepatic fibrosis and liver cancer. However, Dectin-1 deletion exacerbates liver fibro-inflammatory disease and accelerates hepatocarcinogenesis. Mechanistically, we found that Dectin-1 protects against chronic liver disease by suppressing TLR4 signaling in hepatic inflammatory and stellate cells. Accordingly, Dectin-1(-/-) mice exhibited augmented cytokine production and reduced survival in lipopolysaccharide (LPS)-mediated sepsis, whereas Dectin-1 activation was protective. We showed that Dectin-1 inhibits TLR4 signaling by mitigating TLR4 and CD14 expression, which are regulated by Dectin-1-dependent macrophage colony stimulating factor (M-CSF) expression. Our study suggests that Dectin-1 is an attractive target for experimental therapeutics in hepatic fibrosis and neoplastic transformation. More broadly, our work deciphers critical cross-talk between pattern recognition receptors and implicates a role for Dectin-1 in suppression of sterile inflammation, inflammation-induced oncogenesis, and LPS-mediated sepsis.

Topics: Animals; Cell Transformation, Neoplastic; Cells, Cultured; Chemokine CCL2; Cytokines; Diethylnitrosamine; Hepatocytes; Humans; Inflammation; Lectins, C-Type; Lipopolysaccharide Receptors; Lipopolysaccharides; Liver; Liver Cirrhosis; Liver Neoplasms; Macrophage Colony-Stimulating Factor; Mice; Mice, Inbred C57BL; Mice, Knockout; Recombinant Proteins; Sepsis; Signal Transduction; Thioacetamide; Toll-Like Receptor 4; Up-Regulation