texas-red and Meniere-Disease

We examined the distribution of gentamicin sulfate in the inner ear after delivery via a chitosan glycerophosphate (CGP) hydrogel system and examined the change in morphology of the hair cells so as to determine how gentamicin affected the function of the inner ear.. A matrix of CGP hydrogel loaded with gentamicin conjugated with Texas Red (GTTR) was injected into the round window niche of the left ear of C57/BL6 mice. The mice were painlessly killed on day 1 or day 7 after injection. Confocal fluorescence microscopy was used to locate the gentamicin in the cochlear and vestibular systems.. In the vestibule, the intensity of GTTR staining in the hair cells of the macula of the saccule on day 1 was significantly stronger than that on day 7, and the number of hair cell bundles on top of the cuticular plate on day 7 was obviously decreased in comparison to that on day 1. In the cochlea, the intensity of GTTR staining in the basal turn was significantly stronger than that in the medial turn on both day 1 and day 7. Negligible fluorescence was observed in the apical turn on both day 1 and day 7. Less-intense GTTR staining was detected on day 7 than on day 1 in both the basal turn and the medial turn. There was some outer hair cell loss in the basal turn on day 7, and no hair cell loss in the medial and apical turns at any time point.. Gentamicin is distributed in the inner ear via the CGP hydrogel delivery system in a time-dependent and basal-to-apical manner. This finding implies that the vestibule and the basal turn may retain more gentamicin for a longer period than do other sites in the inner ear. These two characteristics may account for the high-frequency hearing loss and vestibular dysfunction seen with use of this system to deliver gentamicin into the inner ear.

Topics: Animals; Anti-Bacterial Agents; Chitosan; Delayed-Action Preparations; Drug Delivery Systems; Ear, Inner; Fluorescent Dyes; Gentamicins; Hair Cells, Auditory; Hair Cells, Vestibular; Hydrogel, Polyethylene Glycol Dimethacrylate; Meniere Disease; Mice; Mice, Inbred C57BL; Xanthenes

The distribution of gentamicin in the inner ear via the endolymphatic sac (ES) or round window (RW) routes was investigated.. Experimental study.. A fluorescent gentamicin-Texas Red conjugate (GTTR) was adopted to visualize the gentamicin. Adult guinea pigs were treated with GTTR applied to the ES or RW, the animals were allowed to recover for 3 days, then confocal microscopy was used to observe and quantify GTTR distributions in cochlear and vestibular sensory epithelium.. When GTTR was applied via the ES, strong GTTR labeling was observed in the vestibule while little GTTR was detected in the cochlea (P < .0001). However, distinct GTTR fluorescence was observed in the cochlea and vestibule following RW application (P = .7967). There was less GTTR labeling in cochlea via ES application than through RW administration (P < .0001).. ES drug application may be preferable for the treatment of intractable Meniere's disease. Laryngoscope, 2010.

Topics: Administration, Topical; Animals; Anti-Bacterial Agents; Cochlea; Endolymphatic Sac; Evoked Potentials, Auditory, Brain Stem; Gentamicins; Guinea Pigs; Hair Cells, Auditory; Meniere Disease; Microscopy, Confocal; Round Window, Ear; Xanthenes