tetramethylrhodamine and Virus-Diseases

tetramethylrhodamine has been researched along with Virus-Diseases* in 1 studies

Other Studies

1 other study(ies) available for tetramethylrhodamine and Virus-Diseases

Article	Year
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens. Journal of virological methods, 2009, Volume: 162, Issue:1-2 Tetramethylrhodamine (TAMRA) and black hole quencher 1 (BHQ1) quenched probes and five one-step RT-PCR kits were evaluated in TaqMan real-time RT-PCR assays for detection of respiratory pathogens. The intra-assay variability of the BHQ1 probes were 1.2-2.8-fold lower than those of the TAMRA probes. All kits amplified the specific targets, but differed in their sensitivity by up to 3 orders of magnitude. The AgPath-ID kit provided the best overall performance for all assay targets. Topics: Bacteria; Bacterial Infections; DNA Probes; Fluorescent Dyes; Humans; Reagent Kits, Diagnostic; Respiratory System; Reverse Transcriptase Polymerase Chain Reaction; Rhodamines; Taq Polymerase; Virus Diseases; Viruses	2009

Article

Year

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens.

Journal of virological methods, 2009, Volume: 162, Issue:1-2

Tetramethylrhodamine (TAMRA) and black hole quencher 1 (BHQ1) quenched probes and five one-step RT-PCR kits were evaluated in TaqMan real-time RT-PCR assays for detection of respiratory pathogens. The intra-assay variability of the BHQ1 probes were 1.2-2.8-fold lower than those of the TAMRA probes. All kits amplified the specific targets, but differed in their sensitivity by up to 3 orders of magnitude. The AgPath-ID kit provided the best overall performance for all assay targets.

Topics: Bacteria; Bacterial Infections; DNA Probes; Fluorescent Dyes; Humans; Reagent Kits, Diagnostic; Respiratory System; Reverse Transcriptase Polymerase Chain Reaction; Rhodamines; Taq Polymerase; Virus Diseases; Viruses

2009