tetracycline and Wounds-and-Injuries

Topics: Calcium Chloride; Carbon Dioxide; Debridement; Diagnosis, Differential; Epinephrine; Heart Arrest; Humans; Hydrogen Peroxide; Hypnotics and Sedatives; Immunoglobulins; Immunotherapy; Intensive Care Units; Metabolic Diseases; Oxygen; Penicillins; Positive-Pressure Respiration; Spasm; Tetanus; Tetanus Antitoxin; Tetracycline; Tracheotomy; Urban Population; Wounds and Injuries

Topics: Anti-Inflammatory Agents; Bromelains; Clinical Trials as Topic; Drug Combinations; Edema; Female; Genital Diseases, Female; Hematoma; Humans; Intestinal Mucosa; Male; Phlebitis; Surgical Wound Infection; Tetracycline; Wounds and Injuries

Designing an antibacterial agent with a suitable water vapor permeability, good mechanical properties, and controlled antibiotic release is a promising method for stopping bacterial infection in wound tissue. In this respect, this work aims to prepare novel flexible polymeric hydrogel films via integrating UiO-66 into the polymeric carboxymethyl cellulose (CMC) hydrogel for improving the mechanical and antibiotic release performances. First, we performed a green hydrothermal synthetic method to synthesis UiO-66 and followed by encapsulating Tetracycline (TC) through immersion in its aqueous solution. Also, the casting technique was utilized to integrate different concentrations of the TC-encapsulated UiO-66 (TC@UiO-66, 5% to 15%) in the polymeric CMC matrix (CMC/TC@UiO-66) cross-linked by citric acid and plasticized by glycerol. The release performance showed a low initial burst release with a controlled release over 72 h in the artificial sweat and simulated wound exudate (PBS, pH 7.4) media. The in vitro cytotoxicity and antibacterial activity results revealed a good cytocompatibility toward Human skin fibroblast (HFF-1) cells and a significant activity against both E. coli and S. aureus with 1.3 and 1.7 cm inhibition zone, respectively. The obtained results recommend CMC/TC@UiO-66 films as a potential antibacterial wound dressing.

Topics: Anti-Bacterial Agents; Bandages; Carboxymethylcellulose Sodium; Cell Death; Cell Line; Cell Survival; Drug Liberation; Escherichia coli; Humans; Hydrogels; Hydrogen-Ion Concentration; Microbial Sensitivity Tests; Nanocomposites; Organometallic Compounds; Phthalic Acids; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Tensile Strength; Tetracycline; Wounds and Injuries

The intricate wound repair process involves the interplay of numerous cells and proteins. Using a porcine full-thickness wound (FTW) healing model, we hypothesized that the ex vivo gene transfer of vascular endothelial growth factor (VEGF)-transfected basal keratinocyte (KC) cell suspensions may generate cross-talk and induce matrix formation, angiogenesis, and accelerated healing. Moreover, to regulate overexpression of isoform 165 of VEGF and its effect on healing, we introduced a tetracycline (TC)-inducible gene switch in the expression plasmid. Autologous basal KCs were cultivated from the porcine donor and transfected using cationic liposomes. A dose-response curve was established to determine optimal activation of the gene switch by TC. In vivo, FTWs were treated with VEGF-transfected KCs and controls. Wound fluids were collected daily and examined using enzyme-linked immunosorbent assay. Biopsies were evaluated using hematoxylin and eosin and immunostaining for fibronectin, CD144, and lectin BS-1. In vitro, highest regulable VEGF165-expression was obtained with 1 microg/mL of TCs. In vivo, after induction of the gene switch by adding 1 microg/mL of TCs to the FTW, we obtained upregulated VEGF165 levels and enhanced fibronectin deposition and found more endothelial cell tubular formations and higher rates of reepithelialization than in controls. This ex vivo gene transfer model may serve as a platform for vascular induction in full-thickness tissue repair.

Topics: Animals; Anti-Bacterial Agents; Cells, Cultured; Disease Models, Animal; Endothelial Cells; Extracellular Matrix; Fibronectins; Gene Expression; Keratinocytes; Neovascularization, Physiologic; Swine; Tetracycline; Transfection; Transplantation, Autologous; Vascular Endothelial Growth Factor A; Wound Healing; Wounds and Injuries

We recently developed a new tetracycline-inducible gene switch employing the tetracycline operator-containing hCMV major immediate-early promoter and the tetracycline repressor, tetR, rather than the previously used tetR-mammalian cell transcription factor fusion derivatives.. The present study demonstrates that this tetR-mediated transcription repression system can function as a powerful gene switch for On-and-Off regulation of therapeutic gene expression in ex vivo gene transfer protocols. Firstly, for achieving regulated gene expression in a localized tissue environment, R11/OEGF cells, a stable line that expresses hEGF under the control of the tetR-mediated transcription repression switch, were transplanted into porcine full-thickness wounds enclosed by wound chambers.. By topically applying tetracycline in wound chambers at various concentrations or at different time points post-transplantation, the levels and timing of hEGF expression in transplanted wounds could be reversibly regulated by tetracycline. Over 3000-fold induction in hEGF expression was achieved in the local wound microenvironment. Secondly, R11/OEGF cells were intramuscularly injected into NCr outbread nude mice to test the efficacy of intermittent systemic gene delivery of a soluble peptide(s).. Basal circulating hEGF was undetectable and induced up to at least 1,500-fold after administration of tetracycline. Furthermore, the timing and duration of hEGF expression could be finely adjusted by the presence or the absence of tetracycline in the drinking water.

Topics: Animals; Anti-Bacterial Agents; Cell Line, Tumor; Cytomegalovirus; Epidermal Growth Factor; Female; Gene Expression; Gene Transfer Techniques; Humans; Male; Mice; Mice, Nude; Osteosarcoma; Promoter Regions, Genetic; Skin; Sus scrofa; Tetracycline; Transgenes; Wounds and Injuries

This article describes the first (to our knowledge) tetracycline-inducible regulatory system that demonstrates that the tetracycline repressor (tetR) alone, rather than tetR-mammalian cell transcription factor fusion derivatives, can function as a potent trans-modulator to regulate gene expression in mammalian cells. With proper positioning of tetracycline operators downstream of the TATA element and of human epidermal growth factor (hEGF) as a reporter, we show that gene expression from the tetracycline operator-bearing hCMV major immediate-early enhancer-promoter (pcmvtetO) can be regulated by tetR over three orders of magnitude in response to tetracycline when (1) the reporter was cotransfected with tetR-expressing plasmid in transient expression assays, and (2) the reporter unit was stably integrated into the chromosome of a tetR-expressing cell line. This level of tetR-mediated inducible gene regulation is significantly higher than that of other repression-based mammalian cell transcription switch systems. In an in vivo porcine wound model, close to 60-fold tetR-mediated regulatory effects were detected and it was reversed when tetracycline was administered. Collectively, this study provides a direct implementation of this tetracycline-inducible regulatory switch for controlling gene expression in vitro, in vivo, and in gene therapy.

Topics: Animals; Base Sequence; Chlorocebus aethiops; Cytomegalovirus; Epidermal Growth Factor; Female; Gene Expression Regulation; Gene Transfer Techniques; Genes, Immediate-Early; Genes, Reporter; HeLa Cells; Herpes Simplex Virus Protein Vmw65; Humans; Molecular Sequence Data; Operator Regions, Genetic; Osteosarcoma; Recombinant Fusion Proteins; Repressor Proteins; Swine; TATA Box; Tetracycline; Transcription, Genetic; Tumor Cells, Cultured; Vero Cells; Wounds and Injuries

Actinomycotic infections are unusual, but the actual incidence is likely to be significantly higher than records indicate. The disease may complicate trauma of many types to the respiratory and digestive tracts, including operative procedures. This possibility should encourage more frequent use of anaerobic cultures in inflammatory diseases, particularly posttraumatic, and should prompt consideration of actinomycosis in the differential diagnosis of infections, especially in the cervicofacial area. We report four cases that demonstrate the variable course of this infection. Treatment is highly successful with appropriate use of antibiotics and surgery. A plea is made to use the least expensive, effective antibiotic in view of the prolonged course of therapy that is necessary to eradicate this infection.

Topics: Abscess; Actinomycosis, Cervicofacial; Aged; Ampicillin; Anti-Bacterial Agents; Carcinoma, Squamous Cell; Cephalosporins; Female; Humans; Laryngeal Neoplasms; Male; Middle Aged; Parotid Gland; Penicillin G; Tetracycline; Wounds and Injuries

The ecology of 350 strains of group F streptococci isolated from clinical material over a six-year period is described. The respiratory tract, particularly the throat, yielded the largest number of isolates. Wound swabs, mainly appendectomy, ranked second as a source of this organism, followed closely by the genitourinary tract. A significant proportion of strains was recovered from gastrointestinal sites. Dental abscess yielded several strains, often in pure culture, and the external auditory canal was identified as minor locale of the group F streptococcus. A few isolates were also obtained from blood, cerebrospinal fluid, and miscellaneous tissues.

Topics: Digestive System; Ear, External; Ecology; Erythromycin; Humans; Penicillins; Periapical Abscess; Respiratory System; Streptococcus; Tetracycline; Urogenital System; Wounds and Injuries

The effectiveness of prophylactic antibiotics is dependent upon both the antimicrobial potency of the drug and the time at which it is first administered. Effectiveness is progressively lost when therapy is delayed, and it is generally recognized that drugs administered only 3-4 hours after contamination of a wound are largely without benefit. Another factor potentially influencing effectiveness in the traumatized patient is the considerable variation in the rate in which various antibiotics penetrate into the interstitial fluid compartment of surgical or traumatic wounds. The present study investigated the effect of route and method of administration of ampicillin, gentamicin, clindamycin, tetracycline, and cephalothin on subsequent wound fluid concentrations. Equivalent doses of each antibiotic were administered by either intermittent intravenous bolus (IV Push), continuous IV infusion (IV Cont) or intermittent intramuscular injection (IM). Peripheral blood and wound tissue fluid from previously implanted stainless steel cylinders were sampled sequentially during an 18 to 24 hour period and assayed for concentration of the antibiotic. Each antibiotic had a different pattern of distribution between serum and wound tissue compartments, but in general, the IV Push method showed comparable levels in wound fluid 4-12 times faster than the IV Cont method. After 12 hours, the highest sustained antibiotic concentrations in wound fluid was usually achieved with the IM route. These data suggest that the earliest and most sustained levels of antibiotic in wound tissue fluid can be achieved by a simultaneous IV Push and IM injection of the drug followed by intermittent IM injections in the normotensive patient or by an IV Push followed by IV Cont administration for patients in shock. These techniques are recommended when it is not possible to administer prophylactic antibiotics before bacterial contamination has occurred, such as regularly occurs in the traumatized patient, especially when treatment is delayed.

Topics: Ampicillin; Animals; Anti-Bacterial Agents; Cephalothin; Clindamycin; Dogs; Exudates and Transudates; Female; Gentamicins; Infusions, Parenteral; Injections, Intramuscular; Male; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Animals; Anti-Bacterial Agents; Bacterial Infections; Cat Diseases; Cats; Dog Diseases; Dogs; Drug Resistance, Microbial; Drug Therapy, Combination; Endocarditis, Bacterial; Kanamycin; Microbial Sensitivity Tests; Pneumonia; Streptomycin; Tetracycline; Time Factors; Wounds and Injuries

Topics: Acute Disease; Anti-Bacterial Agents; Bone Diseases; Clindamycin; Drug Therapy, Combination; Humans; Lincomycin; Moscow; Orthopedics; Penicillin G; Penicillin Resistance; Penicillins; Postoperative Complications; Pseudomonas Infections; Staphylococcal Infections; Streptomycin; Tetracycline; Wound Infection; Wounds and Injuries

The prevalence of tetracycline-resistant beta-haemolytic streptococci in South-west Essex has been recorded over the past 10 years. It has fallen from a peak of 35% in 1965 to a level of 9.2% in 1972. Ear infections no longer provide the highest incidence of these organisms; vaginal, perineal, and skin infections now seem to be of greater relative importance but throat swabs still provide the greatest actual number of isolations. Erythromycin-resistant strains are still rare.

Topics: Drug Resistance, Microbial; Ear; Ear Diseases; England; Erythromycin; Female; Humans; Nose; Paranasal Sinuses; Perineum; Pharynx; Skin Diseases; Sputum; Streptococcal Infections; Streptococcus pyogenes; Tetracycline; Vaginal Diseases; Vulva; Wounds and Injuries

Topics: Burns; Chloramphenicol; Drug Resistance, Microbial; Drug Synergism; Humans; Kanamycin; Melioidosis; Microbial Sensitivity Tests; Novobiocin; Pseudomonas; Sulfonamides; Tetracycline; Time Factors; Toluene; Wounds and Injuries

Topics: Animals; Anti-Bacterial Agents; Bacitracin; Chloramphenicol; Collagen; Fibrin; Hydroxyproline; Penicillins; Rats; Streptomycin; Tetracycline; Time Factors; Wound Healing; Wounds and Injuries

Topics: Animals; Klebsiella Infections; Proteus Infections; Pseudomonas Infections; Rats; Solutions; Staphylococcal Infections; Streptomycin; Tetracycline; Therapeutic Irrigation; Vancomycin; Wounds and Injuries

Topics: Aged; Agglutination Tests; Ampicillin; Cellulitis; Cephalosporins; Chloramphenicol; Diabetes Complications; Erythromycin; Humans; Immunodiffusion; Male; Neomycin; Pasteurella; Pasteurella Infections; Penicillin G; Penicillin Resistance; Sigmoid Neoplasms; Sulfonamides; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Adult; Bacteria; Cephalothin; Humans; Penicillin G; Retrospective Studies; Staphylococcal Infections; Staphylococcus; Surgical Wound Infection; Tetracycline; Wounds and Injuries

Topics: Adult; Aged; Amobarbital; Child; Chloramphenicol; Humans; Middle Aged; Neostigmine; Oxytetracycline; Phenytoin; Tetanus; Tetanus Antitoxin; Tetanus Toxoid; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Animals; Bacteria; Gas Gangrene; Humans; Immunization, Passive; Liver Extracts; Male; Neomycin; Penicillin G Procaine; Rats; Tetracycline; Time Factors; Wounds and Injuries

Topics: Animals; Guinea Pigs; Humans; Staphylococcal Infections; Surgical Wound Infection; Tetracycline; Wounds and Injuries

Topics: Humans; Suture Techniques; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Adult; Anti-Bacterial Agents; Female; Humans; Male; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Child; Child, Preschool; Chlortetracycline; Female; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Infant, Premature, Diseases; Oxytetracycline; Pregnancy; Tetracycline; Tooth Discoloration; Tooth, Deciduous; Twins; Wounds and Injuries

Topics: Adolescent; Anti-Bacterial Agents; Carpal Tunnel Syndrome; Drug Therapy; Hand; Hematoma; Humans; Infections; Penicillins; Protein Synthesis Inhibitors; Streptomycin; Surgical Procedures, Operative; Tetracycline; Wounds and Injuries; Wrist; Wrist Joint

Topics: Anti-Bacterial Agents; Debridement; Humans; Penicillins; Preventive Medicine; Protein Synthesis Inhibitors; Tetanus; Tetanus Antitoxin; Tetanus Toxoid; Tetracycline; Wounds and Injuries

Topics: Anti-Bacterial Agents; Fractures, Bone; Gangrene; Hospitals, General; Injections, Intravenous; Orthopedics; Osteomyelitis; Rolitetracycline; Tetracycline; Toxicology; Ulcer; Wounds and Injuries

Topics: Adolescent; Anti-Bacterial Agents; Bacillus; Child; Chloramphenicol; Cross Infection; Hospitals; Humans; India; Infant; Micrococcus; Nose; Orthopedics; Penicillins; Pharynx; Pneumococcal Infections; Pseudomonas Infections; Staphylococcal Infections; Streptomycin; Tetracycline; Wounds and Injuries

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Arthritis; Blood Chemical Analysis; Dogs; Injections, Intra-Arterial; Injections, Intravenous; Joints; Oxytetracycline; Penicillins; Research; Synovial Fluid; Synovial Membrane; Tetracycline; Wounds and Injuries

Topics: Anti-Bacterial Agents; Humans; Neomycin; Protein Synthesis Inhibitors; Psychotherapy; Tetracycline; Wounds and Injuries

Topics: Anti-Bacterial Agents; Fluorescence; Protein Synthesis Inhibitors; Tetracycline; Ulcer; Wound Healing; Wounds and Injuries

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Dermatologic Agents; Humans; Penicillins; Tetracycline; Wounds and Injuries

Topics: Cicatrix; Cornea; Corneal Injuries; Humans; Protein Synthesis Inhibitors; Research; Tetracycline; Wounds and Injuries