tetracycline and Toxoplasmosis--Animal

tetracycline has been researched along with Toxoplasmosis--Animal* in 3 studies

Reviews

1 review(s) available for tetracycline and Toxoplasmosis--Animal

Article	Year
Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii. Parasitology, 2014, Volume: 141, Issue:11 The dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput. Topics: Animals; Gene Expression Regulation; Genes, Essential; Genes, Protozoan; Mutation; Phenotype; Tetracycline; Toxoplasma; Toxoplasmosis, Animal	2014

Article

Year

Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii.

Parasitology, 2014, Volume: 141, Issue:11

The dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput.

Topics: Animals; Gene Expression Regulation; Genes, Essential; Genes, Protozoan; Mutation; Phenotype; Tetracycline; Toxoplasma; Toxoplasmosis, Animal

2014

Other Studies

2 other study(ies) available for tetracycline and Toxoplasmosis--Animal

Article	Year
Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion. Science (New York, N.Y.), 2002, Oct-25, Volume: 298, Issue:5594 Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model. Topics: Animals; Calcimycin; Calcium; Cell Line; Cells, Cultured; Exocytosis; Genetic Vectors; Humans; Mice; Movement; Nonmuscle Myosin Type IIA; Organelles; Protozoan Proteins; Tetracycline; Toxoplasma; Toxoplasmosis, Animal; Trans-Activators; Transfection; Transgenes; Virulence; Virulence Factors	2002
[THE EFFECT OF SIMULTANEOUS ADMINISTRATION OF TETRACYCLINE, ASCORBIC ACID, DIACYCLINE AND SULPHOMETOXYDIOZINE ON THE COURSE OF SUBACUTE TOXOPLASMOSIS IN WHITE MICE]. Wiadomosci parazytologiczne, 1964, Volume: 10 Topics: Animals; Antiprotozoal Agents; Ascorbic Acid; Mice; Pharmacology; Research; Tetracycline; Toxoplasmosis; Toxoplasmosis, Animal	1964

Article

Year

Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion.

Science (New York, N.Y.), 2002, Oct-25, Volume: 298, Issue:5594

Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.

Topics: Animals; Calcimycin; Calcium; Cell Line; Cells, Cultured; Exocytosis; Genetic Vectors; Humans; Mice; Movement; Nonmuscle Myosin Type IIA; Organelles; Protozoan Proteins; Tetracycline; Toxoplasma; Toxoplasmosis, Animal; Trans-Activators; Transfection; Transgenes; Virulence; Virulence Factors

2002

[THE EFFECT OF SIMULTANEOUS ADMINISTRATION OF TETRACYCLINE, ASCORBIC ACID, DIACYCLINE AND SULPHOMETOXYDIOZINE ON THE COURSE OF SUBACUTE TOXOPLASMOSIS IN WHITE MICE].

Wiadomosci parazytologiczne, 1964, Volume: 10

Topics: Animals; Antiprotozoal Agents; Ascorbic Acid; Mice; Pharmacology; Research; Tetracycline; Toxoplasmosis; Toxoplasmosis, Animal

1964