tetracycline and Skin-Neoplasms

Topics: Acne Vulgaris; Adult; Aged; Child, Preschool; Female; Gonorrhea; Hirsutism; Humans; Ichthyosis; Infant; Male; Melphalan; Middle Aged; Psoriasis; Sarcoidosis; Skin Diseases; Skin Neoplasms; Syphilis; Tetracycline; United States

Melanoma accounts for 3% of all skin cancers but causes 83% of skin cancer deaths. The first step in treatment of melanoma is the removal of the lesions, usually by surgical excision. Currently most lesions are removed without intraoperative margin control. Post-operative methods inspect 1-2% of the surgical margin and are prone to sampling errors. In this study we evaluate the use of reflectance and fluorescence polarization imaging for the demarcation of melanoma in thick fresh skin excisions.. Pigmented lesions clinically suspicious for melanoma were elliptically excised with proper margins. Elliptical surgical excisions were vertically bisected along the short axis of the specimen into two halves in the middle of the pigmented lesions. The vertically bisected tumor face was imaged. After that, one half of the sample was briefly stained in aqueous 2 mg/ml solution of tetracycline, whereas another half was stained in 0.2 mg/ml aqueous solution of methylene blue. Then both specimens were reimaged. Reflectance images were acquired in the spectral range between 390 and 750 nm. Fluorescence images of the tetracycline-stained tissue were excited at 390 nm and registered between 450 and 700 nm. Fluorescence of the methylene blue-stained samples was excited at 630 nm and registered between 650 and 750 nm. After imaging, the tissue was processed for standard H&E histopathology. The resulting histological and optical images were compared to each other.. Our findings demonstrate that both tetracycline and methylene blue are suitable for imaging dysplastic and benign nevi. Melanoma is better delineated in the samples stained in methylene blue. Accurate and rapid delineation of melanoma in standard fresh surgical excisions appears feasible.

Topics: Adult; Aged; Aged, 80 and over; Chromogenic Compounds; Dysplastic Nevus Syndrome; Fluorescence Polarization; Humans; Image Processing, Computer-Assisted; Lasers; Male; Melanoma; Methylene Blue; Microscopy, Polarization; Middle Aged; Pilot Projects; Skin Neoplasms; Tetracycline

Tetracycline is a photosensitising medication that increases skin vulnerability to UV-related damage.. We prospectively examined tetracycline use and risk of incident melanoma, squamous cell carcinoma (SCC), and basal cell carcinoma (BCC) based on 213 536 participants from the Nurses' Health Study (NHS), NHS2, and Health Professionals Follow-up Study. Information on ever use of tetracycline was asked via questionnaire. Diagnoses of melanoma and SCC were pathologically confirmed.. Tetracycline use was associated with a modestly increased risk of BCC, but was not associated with melanoma or SCC.

Topics: Adult; Carcinoma, Basal Cell; Carcinoma, Squamous Cell; Female; Humans; Incidence; Male; Melanoma; Middle Aged; Photosensitizing Agents; Prospective Studies; Risk; Skin Neoplasms; Tetracycline; United Kingdom

Topics: Administration, Topical; Adult; Aged; Carcinoma, Basal Cell; Dermatitis, Allergic Contact; Dermatitis, Occupational; Diagnosis, Differential; Facial Dermatoses; Female; Humans; Metronidazole; Nursing; Occupational Exposure; Patch Tests; Rosacea; Skin Neoplasms; Tetracycline

(1) Photosensitivity reactions are cutaneous disorders due to exposure to ultraviolet (UV) radiation of natural or artificial origin. They occur or are more prevalent on unprotected skin. The main clinical manifestations are burns, eczema-like rash, urticaria, pigmentation, or onycholysis; (2) Many drugs increase cutaneous sensitivity to UV, sometimes for therapeutic purposes, but it is generally an unwanted effect.

Topics: Aminolevulinic Acid; Amiodarone; Anti-Arrhythmia Agents; Anti-Inflammatory Agents, Non-Steroidal; Cardiovascular Agents; Dermatitis, Photoallergic; Dermatitis, Phototoxic; Dihematoporphyrin Ether; Diuretics; Doxycycline; Drug Hypersensitivity; Drug-Related Side Effects and Adverse Reactions; Eczema; Furocoumarins; Hematoporphyrin Photoradiation; Humans; Methotrexate; Onycholysis; Photochemotherapy; Photosensitivity Disorders; Photosensitizing Agents; Porphyrins; Psychotropic Drugs; Quinolines; Skin; Skin Aging; Skin Diseases; Skin Neoplasms; Skin Pigmentation; Sulfonamides; Sunscreening Agents; Tetracycline; Ultraviolet Rays; Urticaria; Verteporfin

The growth and metastasis of solid tumors relies on the activities of polypeptide growth factors to recruit stromal tissue and expand the tumor mass. Pleiotrophin (PTN) is a secreted growth factor with angiogenic activity that has been found to contribute to the growth and metastasis of tumors including melanoma. Here, we present a gene therapy approach of targeting PTN in established tumors using ribozymes. Tetracycline-regulated ribozyme expression vectors were used to deplete conditionally PTN mRNA from melanoma xenograft tumors in vivo. We found that tetracycline-mediated initiation of ribozyme expression in established tumors reduced further tumor growth. Next, we generated synthetic anti-PTN ribozymes that inhibit PTN-dependent colony formation of cells in soft agar. Intraperitoneal administration of these synthetic ribozymes into nude mice inhibited growth of PTN-positive, subcutaneous melanoma. Furthermore, PTN released from the tumors into the circulation of mice was reduced after ribozyme treatment. These data show that ribozyme targeting of rate-limiting tumor growth factors could provide an efficient tool for cancer therapy and that the efficacy may be reflected in the reduction of the serum levels of the targeted protein, PTN.

Topics: Animals; Carrier Proteins; Cytokines; Gene Expression; Genetic Therapy; Genetic Vectors; Humans; Melanoma; Mice; Mice, Nude; Neovascularization, Pathologic; RNA, Catalytic; RNA, Messenger; Skin Neoplasms; Tetracycline; Transfection

The laminin 5 (Ln-5) gamma2 chain and matrix metalloproteinases (MMPs) MMP-2 and membrane type 1 (MT1)-MMP act cooperatively and are required for highly aggressive melanoma cells to engage in vasculogenic mimicry when cultured on a three-dimensional matrix. Furthermore, generation of Ln-5 gamma2 chain promigratory fragments by MMP-2 and MT1-MMP proteolysis is necessary for an aggressive tumor cell-preconditioned matrix to induce vasculogenic mimicry in poorly aggressive tumor cells. These observations suggest that treatment regimes that specifically target aggressive tumor cells may fail to take into account changes in the extracellular microenvironment that persist after removal or destruction of an aggressive tumor and could result in a recurrence or continuance of the tumor. As a potential therapeutic approach to address this concern, the work presented here measured the molecular consequences of adding a chemically modified tetracycline (CMT-3; COL-3) that inhibits MMP activity to aggressive metastatic melanoma cells in three-dimensional culture. COL-3 inhibited vasculogenic mimicry and the expression of vasculogenic mimicry-associated genes in aggressive cells, as well as the induction of vasculogenic mimicry in poorly aggressive cells seeded onto an aggressive cell-preconditioned matrix. Furthermore, molecular analysis revealed that COL-3 not only inhibited the generation of Ln-5 gamma2 chain promigratory fragments in the aggressive cell-preconditioned matrix but also inhibited the induction of Ln-5 gamma2 chain gene expression in poorly aggressive cells by the aggressive cell-preconditioned matrix. These results suggest that COL-3 (and related chemically modified tetracyclines) may be useful in targeting molecular cues in the microenvironment of aggressive tumors and could potentially be used in a combinatorial manner with other therapies that specifically target and kill aggressive tumor cells.

Topics: Blotting, Western; Cell Adhesion Molecules; DNA Primers; Electrophoresis, Polyacrylamide Gel; Endothelial Growth Factors; Gene Expression Regulation; Humans; Kalinin; Matrix Metalloproteinase 2; Matrix Metalloproteinase Inhibitors; Matrix Metalloproteinases, Membrane-Associated; Melanoma; Metalloendopeptidases; Microscopy, Phase-Contrast; Molecular Mimicry; Neovascularization, Pathologic; Receptor Protein-Tyrosine Kinases; Receptors, Cell Surface; Receptors, TIE; Reverse Transcriptase Polymerase Chain Reaction; Skin Neoplasms; Tetracycline; Tetracyclines; Tumor Cells, Cultured; Uveal Neoplasms; Vascular Endothelial Growth Factor C

The treatment of 15 patients with neoplastic pleurisy and 25 with spontaneous pneumothorax occurring for the second time is described. All were given endopleural tetracycline therapy for symphyseal purposes. In the neoplastic pleurisy cases, the treatment reduced the number of thoracenteses required. In only 1 case did spontaneous pneumothorax recur a short time after treatment.

Topics: Adolescent; Adult; Aged; Breast Neoplasms; Central Nervous System Diseases; Female; Humans; Injections; Lung Neoplasms; Male; Middle Aged; Neoplasms; Pleura; Pleurisy; Pneumothorax; Rectal Neoplasms; Skin Neoplasms; Tetracycline

Topics: Adult; Aged; Female; Griseofulvin; Humans; Male; Mycosis Fungoides; Skin Neoplasms; Tetracycline

Topics: Adult; Cryosurgery; Curettage; Humans; Keratoacanthoma; Male; Methotrexate; Skin Neoplasms; Tetracycline

Topics: Adult; Aged; Eyelids; Head; Humans; Lip Neoplasms; Male; Middle Aged; Neck; Neoplasm Metastasis; Skin Neoplasms; Sulfuric Acids; Surgical Wound Infection; Tetracycline; Thorax

Topics: Animals; Carcinoma, Squamous Cell; Fluorescence; Fluorometry; Kidney; Liver; Methods; Microscopy, Fluorescence; Neoplasms, Experimental; Rats; Skin Neoplasms; Spectrophotometry; Tetracycline; Time Factors

Topics: Aged; Anti-Infective Agents, Local; Diagnosis, Differential; Female; Hematoma; Humans; Male; Melanoma; Middle Aged; Nails; Pseudomonas aeruginosa; Pseudomonas Infections; Skin Diseases; Skin Neoplasms; Tetracycline; Warts

Topics: Child; Coloring Agents; Humans; Leukoplakia; Melanoma; Pathology; Pemphigus; Skin Neoplasms; Staining and Labeling; Tetracycline

Topics: Adolescent; Adult; Aged; Carcinoma, Squamous Cell; Child; Diagnosis, Differential; Fluorescence; Humans; Middle Aged; Skin Diseases; Skin Neoplasms; Tetracycline

Topics: Anti-Bacterial Agents; Coloring Agents; Fluorescent Dyes; Humans; Neoplasms; Skin Neoplasms; Tetracycline