tetracycline and Ischemia

Topics: Cholestyramine Resin; Clindamycin; Clostridium Infections; Enterocolitis, Pseudomembranous; Gastrointestinal Diseases; Humans; Ischemia; Lincomycin; Metals; Staphylococcal Infections; Tetracycline; Vancomycin

Stress pretreatments protect myocardium from ischemic injury. We hypothesized that tetracycline, an antibiotic, may induce a stress response via the inhibition of mitochondrial translation as it induces the cold stress response by translational inhibition in E. coli. If so, tetracycline may protect myocardium from ischemic injury as stress pretreatments do. Thus, we investigated the effects of tetracycline on myocardial ischemia and its association with stress response. In a dog model of acute ischemia, 4mg/kg tetracycline injected 30 min prior to the occlusion improved the functional recovery from stunning of myocardium caused by ischemia. The same dosage of tetracycline dramatically reduced the size of infarct area in murine hearts analyzed by tetrazolium staining. In HeLa cells, tetracycline induced molecules that were increased by cold stress, which suggests that tetracycline may induce a cold stress-like response in mammalian cells. These molecules were also induced by ischemic stress in murine hearts, suggesting that the stress response caused by translational inhibition in mitochondria may be associated with the cardioprotection by tetracycline. Our results suggest that a subclinical dosage of tetracycline may protect heart from ischemic injury. Therefore, tetracycline may be of great use in suppressing the development of infarction caused by myocardial ischemia. This study is also important for providing new insights into the non-antimicrobial effects of tetracycline and its derivatives.

Topics: Animals; Dogs; Heart; HeLa Cells; Hemodynamics; Humans; Ischemia; Mice; Myocardial Ischemia; Myocardium; Reperfusion; Tetracycline

Measurement of the fibrinolytic response of the peritoneum to experimental peritonitis and ischaemia.. Controlled study. Academic surgical unit, UK MATERIAL: Male Wistar rats. Peritoneal injuries were caused in four groups of male Wistar rats (n = 35 in each group): (1) control group ("open and close" laparotomy); (2) bacterial peritonitis (mixed faecal flora); (3) chemical peritonitis (10 mg/ml tetracycline) and; (4) ischaemic peritoneum (ligated peritoneal buttons). Peritoneal biopsy specimens were taken from five animals in each group at seven time intervals and plasminogen activating activity (PAA) measured by fibrin plate assay.. Compared with the control group the three peritoneal injuries produced a uniform reduction in PAA during the first 6 and 12 hours: at 6 hours the median PAA was 0.029 IU/cm2 for bacterial peritonitis, 0.021 IU/cm2 for chemical peritonitis, and 0.05 IU/cm2 for ischaemic peritoneum compared with 0.112 IU/cm2 for the control group; p < 0.001, ANOVA. At 12 hours the median PAA was 0.024 IU/cm2 for bacterial peritonitis, < or = 0.014 IU/cm2 for chemical peritonitis, and 0.05 IU/cm2 for ischaemic peritoneum compared with 0.112 IU/cm2 for the control group; p < 0.001, ANOVA. There then followed a rebound peak in all groups, maximal at 4-7 days, before a return to baseline values at two weeks.. Peritoneal fibrinolysis was appreciably inhibited after three different standardised peritoneal injuries. The data support the hypothesis that there is a single pathophysiological mechanism of adhesion formation.

Topics: Animals; Bacterial Infections; Fibrin; Fibrinolysis; Ischemia; Male; Models, Biological; Peritoneum; Peritonitis; Plasminogen; Postoperative Period; Rats; Rats, Wistar; Tetracycline; Time Factors

Liposomes accumulate is ischaemic rat intestine 24 h after mesenteric occlusion. The accumulation of liposomal components is shown to depend on necrotic or allied alterations in cell integrity/function associated with chronic ischaemia. Moreover, not all liposomal components accumulate at sites of infarction. It is concluded therefore that liposomes are likely to be of little value in the diagnosis and management of ischaemic disorders.

Topics: Animals; Etidronic Acid; Hydrocortisone; Intestines; Ischemia; Liposomes; Male; Necrosis; Pharmaceutical Vehicles; Rats; Tetracycline; Time Factors

The action of Mercurascan and Tetracycline on ischaemic liver in mice was investigated. The effect was reflected in different immunogenicity (in allotrasplantation reaction) of liver extracts 1 mg. membrane fraction) derived from treated and normal organs in the donor--recipient strain combination B10--B10.LP. In the recipients treated with a single administration of extract from ischaemic liver the survival time of skin grafts was shortened as compared to the untreated control group and the control group given normal liver extract. Immunogenicity of the liver extracts from Mercurascan- or Tetracycline-treated mice was diminished.

Topics: Animals; Antibody Formation; Fluoresceins; Ischemia; Isoantigens; Liver; Liver Extracts; Mice; Mice, Inbred C57BL; Organomercury Compounds; Skin Transplantation; Tetracycline; Transplantation, Homologous

A disease characterized by segments of ischaemic small intestine has been recognized in norther Thailand over the past decade. The clinical features and appearance of the diseased intestime are described. Most of the patients were treated by surgical resection of the affected bowel. The overall mortality was 14 per cent. Recently, some patients have been successfully treated by non-surgical means. The aetiology of the disease is still unknown.

Topics: Adolescent; Adult; Child; Child, Preschool; Diarrhea; Enteritis; Feces; Female; Gastrointestinal Hemorrhage; Humans; Hydrocortisone; Infant; Intestinal Mucosa; Intestine, Small; Ischemia; Male; Parasite Egg Count; Tetracycline; Thailand

Topics: Administration, Topical; Adult; Diabetes Complications; Diabetes Mellitus; Diabetic Coma; Foot Dermatoses; Gangrene; Humans; Hyperglycemia; Insulin; Ischemia; Skin Ulcer; Tetracycline; Tetracyclines

Topics: Age Factors; Animals; Cartilage, Articular; Dogs; Epiphyses; Femur Head; Femur Neck; Hip; Hip Joint; Humans; Ischemia; Necrosis; Osteochondritis; Prognosis; Rabbits; Radiography; Swine; Tetracycline

Topics: Animals; Calcification, Physiologic; Calcium; Female; Fluorescence; Ischemia; Kidney; Male; Microradiography; Organ Size; Osteogenesis; Rats; Tetracycline

Topics: Animals; Chlorpromazine; Cysteine; Injections, Intraperitoneal; Ischemia; Liver Diseases; Necrosis; Rats; Tetracycline

Topics: Animals; Bone and Bones; Bone Development; Bone Marrow; Dogs; Ischemia; Microscopy, Fluorescence; Pubic Bone; Tetracycline

Topics: Animals; Cats; Fluorescent Dyes; Humans; Ischemia; Methods; Myocardial Infarction; Tetracycline; Ultraviolet Rays

Topics: Animals; Fluorescence; Ischemia; Kidney Transplantation; Rabbits; Tetracycline

Topics: Adrenal Gland Diseases; Animals; Fluorescence; Histological Techniques; Infarction; Ischemia; Rats; Tetracycline

Topics: Acute Kidney Injury; Animals; Chromates; Female; Glomerular Filtration Rate; Ischemia; Nitrates; Rats; Serine; Tetracycline

Topics: Adrenocorticotropic Hormone; Ascorbic Acid; Blood Coagulation Disorders; Diagnosis; Drug Therapy; Fibrinolysis; Humans; Ischemia; Necrosis; Pathology; Prednisone; Promethazine; Purpura; Purpura Fulminans; Tetracycline

Topics: Adrenocorticotropic Hormone; Ascorbic Acid; Blood Coagulation Disorders; Diagnosis; Drug Therapy; Fibrinolysis; Humans; Ischemia; Necrosis; Pathology; Prednisone; Promethazine; Purpura; Purpura Fulminans; Tetracycline

Topics: Fluorescence; Infarction; Ischemia; Liver Circulation; Liver Diseases; Microscopy; Microscopy, Fluorescence; Pathology; Protein Synthesis Inhibitors; Rats; Research; Tetracycline

Topics: Ammonium Compounds; Chlorides; Fluorescence; Heparin Antagonists; Ischemia; Kidney; Kidney Diseases; Kidney Tubules; Mercury; Necrosis; Pathology; Potassium; Quaternary Ammonium Compounds; Rats; Research; Serine; Tetracycline; Toxicology

Topics: Anti-Bacterial Agents; Chloramphenicol; Ischemia; Liver Diseases; Penicillins; Rabbits; Research; Tetracycline

Topics: Estradiol; Fluorescence; Ischemia; Kidney Diseases; Pathology; Pharmacology; Physiology; Pituitary Hormones; Pituitary Hormones, Posterior; Rats; Research; Tetracycline

Topics: Anti-Bacterial Agents; Burns; Fluorescence; Humans; Inflammation; Ischemia; Myocardial Infarction; Necrosis; Neoplasms; Tetracycline