tetracycline and Fractures--Bone

Compared with the current standard of implanting bone anabolics for fracture repair, bone fracture-targeted anabolics would be more effective, less invasive, and less toxic and would allow for control over what phase of fracture healing is being affected. We therefore sought to identify the optimal bone-targeting molecule to allow for systemic administration of therapeutics to bone fractures.. We found that many bone-targeting molecules exist, but most have been developed for the treatment of bone cancers, osteomyelitis, or osteoporosis. There are a few examples of bone-targeting ligands that have been developed for bone fractures that are selective for the bone fracture over the body and skeleton. Acidic oligopeptides have the ideal half-life, toxicity profile, and selectivity for a bone fracture-targeting ligand and are the most developed and promising of these bone fracture-targeting ligands. However, many other promising ligands have been developed that could be used for bone fractures.

Topics: Alkaline Phosphatase; Amino Acids, Acidic; Anabolic Agents; Diphosphonates; Drug Delivery Systems; Durapatite; Fracture Healing; Fractures, Bone; Humans; Immunoglobulin G; Oligopeptides; Recombinant Fusion Proteins; Tartrate-Resistant Acid Phosphatase; Tetracycline

Topics: Angiography; Animals; Blood Vessels; Bone and Bones; Bone Development; Bone Marrow; Calcification, Physiologic; Dogs; Embolism; Femur; Fractures, Bone; Methods; Microscopy, Fluorescence; Necrosis; Periosteum; Rabbits; Regional Blood Flow; Staining and Labeling; Tetracycline; Tibia; Wound Healing

Idiopathic osteoporosis (IOP) in premenopausal women is characterized by fragility fractures at low or normal bone mineral density (BMD) in otherwise healthy women with normal gonadal function. Histomorphometric analysis of transiliac bone biopsy samples has revealed microarchitectural deterioration of cancellous bone and thinner cortices. To examine bone material quality, we measured the bone mineralization density distribution (BMDD) in biopsy samples by quantitative backscattered electron imaging (qBEI), and mineral/matrix ratio, mineral crystallinity/maturity, relative proteoglycan content, and collagen cross-link ratio at actively bone forming trabecular surfaces by Raman microspectroscopy and Fourier transform infrared microspectroscopy (FTIRM) techniques. The study groups included: premenopausal women with idiopathic fractures (IOP, n = 45), or idiopathic low BMD (Z-score ≤ -2.0 at spine and/or hip) but no fractures (ILBMD, n = 19), and healthy controls (CONTROL, n = 38). BMDD of cancellous bone showed slightly lower mineral content in IOP (both the average degree of mineralization of cancellous bone [Cn.Ca(Mean) ] and mode calcium concentration [Cn.Ca(Peak) ] are 1.4% lower) and in ILBMD (both are 1.6% lower, p < 0.05) versus CONTROL, but no difference between IOP and ILBMD. Similar differences were found when affected groups were combined versus CONTROL. The differences remained significant after adjustment for cancellous mineralizing surface (MS/BS), suggesting that the reduced mineralization of bone matrix cannot be completely accounted for by differences in bone turnover. Raman microspectroscopy and FTIRM analysis at forming bone surfaces showed no differences between combined IOP/ILBMD groups versus CONTROL, with the exceptions of increased proteoglycan content per mineral content and increased collagen cross-link ratio. When the two affected subgroups were considered individually, mineral/matrix ratio and collagen cross-link ratio were higher in IOP than ILBMD. In conclusion, our findings suggest that bone material properties differ between premenopausal women with IOP/ILBMD and normal controls. In particular, the altered collagen properties at sites of active bone formation support the hypothesis that affected women have osteoblast dysfunction that may play a role in bone fragility.

Topics: Adult; Bone and Bones; Bone Density; Bone Matrix; Female; Fractures, Bone; Humans; Microspectrophotometry; Middle Aged; Osteoblasts; Osteogenesis; Osteoporosis; Premenopause; Prospective Studies; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Tetracycline

Topics: Animals; Bony Callus; Calcium; Fractures, Bone; Male; Phenytoin; Rats; Tetracycline; Wound Healing

Topics: Animals; Bone Regeneration; Bone Resorption; Casts, Surgical; Femur; Fractures, Bone; Male; Organ Size; Rabbits; Tetracycline; Tibia; Wound Healing

Topics: Adult; Chromium Alloys; Diplopia; Fractures, Bone; Humans; Male; Orbit; Penicillin G Procaine; Prostheses and Implants; Radiography; Surgical Mesh; Tetracycline

Topics: Age Factors; Animals; Epiphyses; Fractures, Bone; Fractures, Closed; Radiography; Rats; Tetracycline; Tibia; Tibial Fractures; Wound Healing

Topics: Adult; Animals; Bone and Bones; Bony Callus; Fractures, Bone; Humans; Male; Rabbits; Stress, Mechanical; Tetracycline; Tibia; Time Factors; Wound Healing

Topics: Anesthetics, Local; Calcaneus; Drug Combinations; Foot Injuries; Fractures, Bone; Fractures, Open; Hand Injuries; Humans; Injections; Metatarsus; Morpholines; Oleandomycin; Surgical Wound Infection; Tetracycline

Topics: Animals; Fractures, Bone; Fractures, Open; Hematopoiesis; Rabbits; Tetracycline

Topics: Anti-Bacterial Agents; Erythromycin Ethylsuccinate; Fractures, Bone; Humans; Injections, Intra-Arterial; Joint Dislocations; Kanamycin; Oleandomycin; Osteomyelitis; Streptomycin; Suppuration; Tetracycline

Topics: Animals; Autoradiography; Bony Callus; Calcium; Cell Count; Deficiency Diseases; Fluorescence; Fractures, Bone; Male; Mast Cells; Microradiography; Rats; Sulfur Isotopes; Tetracycline; Vitamin D Deficiency

Topics: Animals; Biopsy; Bone and Bones; Bone Development; Bone Transplantation; Calcium Isotopes; Fractures, Bone; Microscopy, Fluorescence; Pseudarthrosis; Rabbits; Radiography; Tetracycline; Time Factors; Transplantation, Autologous; Ulna

Topics: Animals; Bone Development; Bone Resorption; Fluorescent Dyes; Fractures, Bone; Freeze Drying; Hematoporphyrins; Porphyrins; Rabbits; Rats; Tetracycline; Wound Healing

Topics: Acrylic Resins; Animals; Bone Regeneration; Dogs; Femur; Fractures, Bone; Tetracycline; Wound Healing

Topics: Angiography; Animals; Bone and Bones; Epiphyses; Female; Femoral Fractures; Femur; Fluorescence; Fractures, Bone; Osteotomy; Rabbits; Tetracycline

Topics: Aged; Animals; Bone and Bones; Bone Diseases; Bone Resorption; Cortisone; Female; Fluorides; Fractures, Bone; Heparin; Humans; Male; Osteitis Deformans; Osteoporosis; Otosclerosis; Phosphates; Rats; Sodium; Tetracycline

Topics: Animals; Bone and Bones; Bone Regeneration; Fluorescence; Fractures, Bone; Male; Osteomyelitis; Rabbits; Tetracycline

Topics: Animals; Bone and Bones; Bone Regeneration; Fibula; Fractures, Bone; Haversian System; Humans; Rabbits; Rats; Tetracycline; Tibial Fractures; Wound Healing

Topics: Bone Regeneration; Fluorescence; Fractures, Bone; Fractures, Ununited; Humans; Microscopy; Microscopy, Fluorescence; Osteogenesis; Osteosclerosis; Tetracycline

Topics: Angiography; Animals; Denervation; Fluorescence; Fractures, Bone; Microradiography; Peripheral Nerves; Rats; Spinal Nerves; Tetracycline; Wound Healing

Topics: Angiography; Bony Callus; Cortisone; Fluorescence; Fractures, Bone; Microradiography; Microscopy; Microscopy, Fluorescence; Oxytetracycline; Pharmacology; Physiology; Rats; Research; Tetracycline; Tibial Fractures

Topics: Anti-Bacterial Agents; Cellulitis; Fracture Fixation; Fractures, Bone; Humans; Injections, Intra-Arterial; Rolitetracycline; Tetracycline; Wound Infection

Topics: Animals; Bone Regeneration; Bone Resorption; Calcium; Fluorescence; Fracture Healing; Fractures, Bone; Periosteum; Pharmacology; Rabbits; Research; Tetracycline; Wound Healing

Topics: Anti-Bacterial Agents; Fractures, Bone; Protein Synthesis Inhibitors; Rats; Research; Tetracycline; Toxicology

Topics: Anti-Bacterial Agents; Fractures, Bone; Humans; Injections, Intra-Arterial; Tetracycline; Wound Infection

Topics: Anti-Bacterial Agents; Fractures, Bone; Gangrene; Hospitals, General; Injections, Intravenous; Orthopedics; Osteomyelitis; Rolitetracycline; Tetracycline; Toxicology; Ulcer; Wounds and Injuries

Topics: Anti-Bacterial Agents; Bone and Bones; Fractures, Bone; Humans; Tetracycline

Topics: Enteritis; Enterocolitis, Pseudomembranous; Femoral Neck Fractures; Femur Neck; Fractures, Bone; Protein Synthesis Inhibitors; Tetracycline