tetracycline has been researched along with Fish-Diseases* in 16 studies
1 review(s) available for tetracycline and Fish-Diseases
1 trial(s) available for tetracycline and Fish-Diseases
15 other study(ies) available for tetracycline and Fish-Diseases
| Article | Year |
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Polystyrene nanoplastics exacerbated the ecotoxicological and potential carcinogenic effects of tetracycline in juvenile grass carp (Ctenopharyngodon idella).
This study aims to evaluate the ecotoxicity effects of single tetracycline (TC) exposure and mixture exposure in presence of polystyrene nanoplastics (PS-NPs, 80 nm) on juvenile Ctenopharyngodon idella. We carried out single and combined exposure of TC (5000 μg/L) and PS-NPs (20, 200, 2000 μg/L) for 7 days. Compared to TC single exposure, co-exposure to PS-NPs and TC significantly changed the levels of antioxidant entities, including T-AOC, SOD, and CAT in the liver and intestine of C. idella, indicating that PS-NPs might enhance the oxidative damage caused by TC. Further, the co-exposure significantly upregulated the mRNA expression levels of MMP2, MMP9, and IL-8 in a concentration-dependent manner in the liver and intestine tissues of C. idella, compared to the control and TC single exposure groups. Moreover, the phylogenetic tree showed that MMP2 and MMP9 in C. idella are relatively conservative, and the mRNA expressions of MMP2 are significantly positively correlated with TGFβ1, IL8, and MMP9 in Liver hepatocellular carcinoma (LIHC) and Colon adenocarcinoma (COAD). The above genes in LIHC and COAD were significantly correlated with various immune cells. Further, histopathological analysis revealed tissue lesions in the intestine and gill of fish in all the exposed groups, compared to the control group. In short, the present study illustrated that the toxicological effects of organic pollutants such as TC could be influenced by the presence of NPs in the C. idella. Topics: Animal Feed; Animals; Carps; Diet; Fish Diseases; Fish Proteins; Microplastics; Phylogeny; Polystyrenes; Tetracycline | 2022 |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration.
Antibiotics are used to treat bacterial infections in fish aquaculture, and these drugs can interact with immune cells/the immune system and potentially leave fish vulnerable to viral, fungal, parasitic, or other bacterial infections. However, the effects of antibiotics on fish immunity have largely been overlooked by the aquaculture industry. We tested, at 12 and 20 °C, whether tetracycline and florfenicol (the most commonly used antibiotics in commercial aquaculture), affected the Atlantic salmon's capacity to respond to bacterial or viral stimulation. Atlantic salmon were acclimated to 12 or 20 °C and fed with tetracycline or florfenicol (100 and 10 mg kg of body weight Topics: Animals; Anti-Bacterial Agents; Fish Diseases; Immunity, Innate; Salmo salar; Tetracycline; Thiamphenicol | 2022 |
Detection of variants of the pRAS3, pAB5S9, and pSN254 plasmids in Aeromonas salmonicida subsp. salmonicida: multidrug resistance, interspecies exchanges, and plasmid reshaping.
The ubiquitous water-borne Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a worldwide disease in fish farms. Plasmids carrying antibiotic resistance genes have already been described for this bacterium. The aim of the present study was to identify and characterize additional multidrug resistance plasmids in A. salmonicida subsp. salmonicida. We sequenced the plasmids present in two multiple antibiotic-resistant isolates using high-throughput technologies. We also investigated 19 other isolates with various multidrug resistance profiles by genotyping PCR and assessed their resistance to tetracycline. We identified variants of the pAB5S9 and pSN254 plasmids that carry several antibiotic resistance genes and that have been previously reported in bacteria other than A. salmonicida subsp. salmonicida, which suggests a high level of interspecies exchange. Genotyping analyses and the antibiotic resistance profiles of the 19 other isolates support the idea that multiple versions of pAB5S9 and pSN254 exist in A. salmonicida subsp. salmonicida. We also identified variants of the pRAS3 plasmid. The present study revealed that A. salmonicida subsp. salmonicida harbors a wide variety of plasmids, which suggests that this ubiquitous bacterium may contribute to the spread of antibiotic resistance genes in the environment. Topics: Aeromonas salmonicida; Animals; Anti-Bacterial Agents; Base Sequence; Canada; Drug Resistance, Multiple, Bacterial; Fish Diseases; Furunculosis; Gene Transfer, Horizontal; Gram-Negative Bacterial Infections; Molecular Sequence Data; Plasmids; Salmon; Sequence Analysis, DNA; Tetracycline | 2014 |
Prevalence of tet gene and complete genome sequencing of tet gene-encoded plasmid (pAHH01) isolated from Aeromonas species in South Korea.
To investigate the tetracycline resistance related to tet genes in Aeromonas isolates collected from water and diseased fish in South Korea.. A total of 34 Aeromonas strains were examined for their susceptibility to tetracycline using the minimum inhibitory concentration (MIC) assay, and the genetic determinants (tetA to E) were analysed. Among these strains, the tetA and tetE genes were predominant (tetA was found in six strains, and tetE was found in nine strains), and 15 strains were tetracycline-resistant by the MIC assay. Additionally, the 8979-bp plasmid that contains the tetE gene was fully sequenced.. These data may be important with regard to the spread and persistence of tetracycline resistance genes in the bacterial populations that are present in aquaculture systems.. Interestingly, no isolate has previously been shown to harbour three tet genes that are mediated by efflux systems, but the tetA, tetD and tetE genes were all isolated from one strain, which had the highest MIC value for tetracycline among the strains analysed in this study. We also investigated the full-length plasmid that encoded the tetE gene from a tetracycline-resistant strain. Topics: Aeromonas; Animals; Anti-Bacterial Agents; Aquaculture; Fish Diseases; Fishes; Microbial Sensitivity Tests; Molecular Sequence Data; Plasmids; Republic of Korea; Sequence Analysis, DNA; Tetracycline; Tetracycline Resistance | 2012 |
Impact of antibiotic treatments on the expression of the R plasmid tra genes and on the host innate immune activity during pRAS1 bearing Aeromonas hydrophila infection in zebrafish (Danio rerio).
The transfer of R plasmids between bacteria has been well studied under laboratory conditions and the transfer frequency has been found to vary between plasmids and under various physical conditions. For the first time, we here study the expression of the selected plasmid mobility genes traD, virB11 and virD4 in the 45 kb IncU plasmid, pRAS1, conferring resistance to tetracycline, trimethoprim and sulphonamide, using an in vivo zebrafish infection- treatment model.. Three days after oral infection of adult zebrafish with Aeromonas hydrophila harboring pRAS1, elevated expression of pro-inflammatory cytokine (TNF α, IL-1β and IL-8) and complement C3 genes in the intestine coincided with disease symptoms. Tetracycline, trimethoprim and an ineffective concentration of flumequine given 48 h prior to sampling, strongly increased expression of plasmid mobility genes, whereas an effective dosage of flumequine resulted in lower levels of mRNA copies of these genes relative to placebo treatment. Following effective treatment with flumequine, and ineffective treatments with a low concentration of flumequine, with trimethoprim or with sulphonamide, the intestinal expression of immune genes was strongly induced compared to placebo treated control fish.. Treatment of zebrafish infected with an antibiotic resistant (TcR, TmR, SuR) A. hydrophila with ineffective concentrations of flumequine or the ineffective antimicrobials tetracycline and trimethoprim strongly induced expression of genes mediating conjugative transfer of the R-plasmid pRAS1. Simultaneously, there was a strong induction of selected inflammatory and immune response genes, which was again evident in fish subjected to ineffective treatment protocols. Our findings point to the essential role of therapeutic practices in escalation or control of antibiotic resistance transfer, and suggest that antibiotic substances, even in sub-inhibitory concentrations, may stimulate innate defenses against bacterial infections. Topics: Aeromonas hydrophila; Animals; Anti-Bacterial Agents; Conjugation, Genetic; Drug Resistance, Multiple, Bacterial; Female; Fish Diseases; Fluoroquinolones; Gene Expression Regulation, Bacterial; Gene Transfer, Horizontal; Gram-Negative Bacterial Infections; Immunity, Innate; Intestines; Male; Metagenome; R Factors; RNA, Ribosomal, 16S; Tetracycline; Trimethoprim; Zebrafish | 2012 |
Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea.
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser(83)→Arg(83) or Ser(83)→Asn(83). We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida. Topics: Aeromonas salmonicida; Animals; Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Bacterial; Environment; Fish Diseases; Fishes; Gram-Negative Bacterial Infections; Microbial Sensitivity Tests; Point Mutation; Polymerase Chain Reaction; Quinolones; Republic of Korea; Sequence Analysis; Tetracycline; Tetracycline Resistance | 2011 |
IncA/C plasmid-mediated florfenicol resistance in the catfish pathogen Edwardsiella ictaluri.
Topics: Animals; Anti-Bacterial Agents; Catfishes; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Edwardsiella ictaluri; Enterobacteriaceae Infections; Fish Diseases; Molecular Sequence Data; Plasmids; Thiamphenicol | 2009 |
Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida.
Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four groups based on the specific tetracycline resistance (tet) gene carried [tet(A) or tet(B)], incompatibility of the plasmid [IncU or other], whether the plasmid carried the IS6100 sequences, the sul1 gene, coding for sulfonamide resistance, the dfrA16 gene, coding for trimethoprim resistance, and/or carried a complete Tn1721, and their ability to transfer their Tc(r) plasmids to an Escherichia coli recipient at 15 degrees C. Five of the isolates, with genetically related Tc(r) plasmids, were able to transfer their plasmids to an E. coli recipient at frequencies ranging from 5.7x10(-4) to 2.8x10(-6) per recipient. The 1992 isolate carried a genetically distinct plasmid, which transferred at a slightly higher rate. The three remaining isolates carried one of two genetically different plasmids, which were unable to transfer to an E. coli recipient. Conjugal transfer at 15 degrees C is the lowest temperature that has been documented in bacteria. Topics: Aeromonas salmonicida; Animals; Anti-Bacterial Agents; Antiporters; Bacterial Proteins; Conjugation, Genetic; DNA Transposable Elements; Drug Resistance, Bacterial; Escherichia coli; Fish Diseases; Genes, Bacterial; Gram-Negative Bacterial Infections; Plasmids; Salmon; Sulfonamides; Temperature; Tetracycline; Tetracycline Resistance; Trimethoprim; Trout | 2005 |
Identification and characterization of sporadic isolates of Streptococcus iniae isolated from humans.
Seven reference strains and seven clinical isolates of Streptococcus iniae, submitted to the Centers for Disease Control and Prevention Streptococcus Reference Laboratory between 2001 and 2004, were successfully identified by a conventional identification system. The seven randomly submitted clinical isolates were sensitive to beta-lactams, macrolides, quinolones, and vancomycin. Two of the seven clinical isolates were resistant to tetracycline. All seven strains grew well and multiplied in a phagocytosis assay. One of the seven randomly submitted strains was more similar to the type strain of S. iniae than to the other six strains. The latter six strains were similar if not identical to representative strains from a cluster of disease in Canada (M. R. Weinstein et al., N. Engl. J. Med. 337:589-594, 1997). Topics: Animals; Anti-Bacterial Agents; Bacterial Typing Techniques; Dolphins; Drug Resistance, Bacterial; Fish Diseases; Fishes; Humans; Microbial Sensitivity Tests; Phenotype; Streptococcal Infections; Streptococcus; Tetracycline | 2005 |
Class 1 integrons mediate antibiotic resistance in the fish pathogen Aeromonas salmonicida worldwide.
The presence of class 1 integrons was investigated in 38 sulfonamide-resistant strains of Aeromonas salmonicida subsp. salmonicida, atypical A. salmonicida and Escherichia coli conjugants with R plasmids originating from A. salmonicida. The strains originated from Finland, France, Japan, Norway, Scotland, Switzerland, and the United States. Additional resistance determinants in strains with class 1 integrons were also determined. Of 21 strains containing a class 1 integron, 19 had a single gene cassette, 1 strain had two cassettes, and 1 strain was found to lack an integrated gene cassette. In the integrons with single cassettes, aadA2 was present in eight strains, dfr16 in five strains, and aadA1 and dfrIIc in three strains each. In the integron with two cassettes, qacG and orfD were present. Tetracycline resistance was observed in 20 of the integron-positive strains, caused by the determinants Tet A and Tet E, in which Tet A frequently was associated with Tn1721. Class 1 integrons seem to be important in mediating antibiotic resistance also in the marine environment. The gene cassettes reported in this study are all described in bacteria associated with humans, and this demonstrates once more how the common gene pool is shared between organisms belonging to different environments. Topics: Aeromonas; Animals; Anti-Bacterial Agents; Blotting, Southern; Conjugation, Genetic; DNA Primers; Drug Resistance; Escherichia coli; Fish Diseases; Fishes; Gram-Negative Bacterial Infections; In Situ Hybridization; Integrins; Microbial Sensitivity Tests; Phenotype; Plasmids; Reverse Transcriptase Polymerase Chain Reaction; Tetracycline | 2001 |
Determination of oxytetracycline in the live fish feed Artemia using high-performance liquid chromatography with ultraviolet detection.
A high-performance liquid chromatographic analytical method was developed for the determination of oxytetracycline in Artemia nauplii. A solid-phase extraction protocol was used to recover oxytetracycline and the internal standard tetracycline, from the Artemia samples. Oxytetracycline was analyzed using a 150 x 4.6 mm I.D. Hypersil-ODS column, a mobile phase of acetonitrile-tetrahydrofuran-0.01 M oxalic acid buffer (pH 3.0) (15:3:82, v/v), and an ultraviolet detection wavelength of 365 nm. The calibration curve of oxytetracycline in Artemia was linear (r2 = 0.9998) from 0.1 to 6.4 micrograms/g of tissue. Using a signal-to-noise ratio of 4:1 the oxytetracycline detection limit was 10 ng/g of tissue. Mean recovery of oxytetracycline amounted to 97%, while intra-assay variability was 1.5%. Quantitative data from an in-vivo feeding study indicated an excellent uptake of oxytetracycline by Artemia, as its levels reached 25.6 micrograms per g of nauplii. Topics: Animal Feed; Animals; Artemia; Chromatography, High Pressure Liquid; Fish Diseases; Fishes; Furans; Liposomes; Oxytetracycline; Tetracycline | 1995 |
Detection of R plasmids in naturally occurring fish-pathogenic bacteria, Edwardsiella tarda.
The conjugative R plasmids were detected from fish-pathogenic Edwardsiella tarda isolated from cultured eels. These R plasmids were resistant to sulfonamide and tetracycline, or sulfonamide, streptomycin, chloramphenicol, tetracycline and kanamycin. There were no inhibitions in growth of phages W-31, P1, T1, T3, T7, and lambda. They were classified into incompatibility group A. Topics: Animals; Chloramphenicol; Conjugation, Genetic; Drug Resistance, Microbial; Enterobacteriaceae; Escherichia coli; Fish Diseases; Kanamycin; Mutation; R Factors; Streptomycin; Sulfonamides; Tetracycline | 1977 |
Effects of chemotherapeutics on bacterial ecology in the water of ponds and the intestinal tracts of cultured fish, ayu (Plecoglossus altivelis).
Drug-resistant gram-negative bacilli conferred with R factors were isolated with high frequencies from the intestinal tracts of ayu (Plecoglossus altivelis) cultured in ponds, in which chemotherapeutics had often been used, and with relatively low frequencies from ayu which received no administration of chemotherapeutics. Drug-resistant bacteria were also isolated at low frequencies from the intestinal tracts of wild ayu in rivers, as well as from the water of ayu-culturing ponds and some of them carried R factors. The drug-resistant bacteria carrying R factors were Aeromonas liquefaciens, Citrobacter, Enterobacter cloacae, Escherichia coli, Hafnia and unidentified strains. All the R factors were classified as the Fi-(F) type, except the two R factors detected in an E. coli strain and in an unidentified strain. Topics: Animals; Chloramphenicol; Drug Resistance, Microbial; Ecology; Enterobacteriaceae; Enterobacteriaceae Infections; Fish Diseases; Fishes; Intestines; Microbial Sensitivity Tests; R Factors; Tetracycline; Water Microbiology | 1975 |
Detection of R factors in naturally occurring Aeromonas salmonicida strains.
R factors were detected in Aeromonas salmonicida strains isolated from diseased salmonid fish. One of such R(+) strains was the one isolated in the United States as early as 1959. Topics: Aeromonas; Animals; Chloramphenicol; Dihydrostreptomycin Sulfate; Drug Resistance, Microbial; Escherichia coli; Fish Diseases; Genetics, Microbial; Microbial Sensitivity Tests; Salmonidae; Sulfathiazoles; Tetracycline | 1971 |
Detection of resistance factors in fish pathogen Aeromonas liquefaciens.
Topics: Aeromonas; Animal Diseases; Animals; Chloramphenicol; Cyprinidae; Drug Resistance, Microbial; Eels; Escherichia coli; Fish Diseases; Fishes; Genetics, Microbial; Microbial Sensitivity Tests; Salmonidae; Streptomycin; Sulfanilamides; Tetracycline; Turtles | 1971 |