tetracycline and Diabetes-Mellitus

Topics: Bone and Bones; Collagen; Diabetes Complications; Diabetes Mellitus; Humans; Microbial Collagenase; Periostitis; Tetracycline

Treating patients with diabetic foot infection (DFI) is challenging because of high rates of antibiotic resistance. Therefore, to administer a suitable antibiotic treatment, it is necessary to know the antibiotic resistance patterns in DFIs.. To explore this question, we selected metagenomic data of 36 tissue samples from patients with DFI in the National Center for Biotechnology Information Sequence Read Archive database.. A total of 229 antibiotic-resistant gene (ARG) subtypes belonging to 20 ARG types were detected. The antibiotic resistome of 229 different genes in the tissue samples of patients with DFI comprised 24 core and 205 accessory resistance genes. Among the core antibiotic resistome, multidrug, tetracycline, macrolide-lincosamide-streptogramin, and beta-lactam resistance genes were the dominant categories. Procrustes analysis indicated that both the microbial community composition and mobile genetic elements (MGEs) were determinants of the ARGs. In the network analysis, 29 species were speculated to be potential hosts of 28 ARGs based on the co-occurrence results. Plasmids and transposons were the most common elements that co-occurred with ARGs.. Our study provided detailed information about antibiotic resistance patterns in DFI, which has practical implications for suggesting a more specific antibiotic choice.

Topics: Anti-Bacterial Agents; Diabetes Mellitus; Diabetic Foot; Drug Resistance, Microbial; Genes, Bacterial; Humans; Tetracycline

To investigate the epidemiology of S. aureus and MRSA nasal carriage among people with diabetes at the Korle Bu Teaching Hospital in Accra, including the prevalence, predictors of carriage, and antibiotic resistance.. This study was cross-sectional, involving 300 diabetes patients and 106 non-diabetic individuals. Swab specimens of the nares were obtained from the participants and bacteriologically-cultured. Identification and characterization of S. aureus and MRSA were based on standard bacteriological methods; antimicrobial susceptibility testing was by the Kirby-Bauer method.. The prevalence of staphylococcal carriage, the diabetes group relative to the non-diabetes group, were 31.0% and 10.4% (S. aureus), and 3.3% and 0.0% (MRSA). Presence of diabetes predisposed to S. aureus carriage, but not MRSA nor coagulase-negative staphylococci (CoNS) carriage (OR = 3.88; p < 0.0001). Colonization with CoNS was protective of S. aureus (OR = 0.039, p < 0.001) and MRSA (OR = 0.115, p = 0.043) colonization among the diabetics. The antimicrobial resistance patterns recorded among the S. aureus isolated from the diabetic individuals relative to the non-diabetics were as follows: penicillin (95% vs. 91%), tetracycline (37% vs. 27%), cotrimoxazole (30% vs. 36%), erythromycin (17% vs. 0%), norfloxacin (13% vs. 0%), clindamycin (12% vs. 0%), gentamicin (9% vs. 0%), fusidic acid (10% vs. 9%), linezolid (4% vs. 0%), and rifampicin (5% vs. 0%). The proportion of multidrug resistant S. aureus was 41% (n = 38) in the diabetes group and 0% in the non-diabetes group; this difference was statistically significant (p = 0.01).. The presence of diabetes predisposed the participants to S. aureus carriage by almost four folds, but not MRSA carriage. Colonization with CoNS was protective of S. aureus and MRSA carriage in the diabetes group. Finally, linezolid remains a good therapeutic agent for anti-MRSA therapy.

Topics: Adolescent; Adult; Anti-Bacterial Agents; Carrier State; Clindamycin; Cross-Sectional Studies; Diabetes Complications; Diabetes Mellitus; Drug Resistance, Multiple, Bacterial; Erythromycin; Female; Fusidic Acid; Gentamicins; Humans; Linezolid; Male; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Middle Aged; Nasal Cavity; Norfloxacin; Penicillins; Rifampin; Staphylococcal Infections; Tetracycline; Trimethoprim, Sulfamethoxazole Drug Combination

Bullous pemphigoid (BP) is the most prevalent autoimmune blistering skin disease in Western countries and in Israel. Initial disease control is achieved in 60-90% of BP patients within 1-4 weeks of corticosteroid therapy. In the remainder of patients, recalcitrant disease is controlled with additional immunosuppressive treatment.. We aimed to evaluate the rate of BP patients who needed adjuvant therapy to achieve initial disease control and to identify potential predictors for recalcitrant disease.. We conducted a retrospective study of newly diagnosed BP patients who were hospitalized at the Tel Aviv Sourasky Medical Center between the years 2008-2014. We performed statistical analyses to assess the association between clinical factors and failure of initial disease control.. Among 114 hospitalized patients with newly diagnosed BP, 1.8% presented with oral mucosa involvement. Seven patients (6.1%) required systemic agents in addition to corticosteroids to achieve disease control. Hypertension (P = 0.048), involvement of the head region (P = 0.042), and metformin treatment (P = 0.02) were significantly more prevalent among patients with recalcitrant disease.. The low frequency of recalcitrant BP (6.1%) and the rarity of involvement of the oral mucosa (1.8%) in this study suggest that Israeli BP patients present a milder phenotype compared to similar patients from other geographic areas. Hypertension, head involvement, and metformin therapy were found to be significantly associated with the need for adjuvant therapy to achieve initial control, suggesting that these parameters may serve as predictors of treatment response in BP.

Topics: Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Anti-Inflammatory Agents; Diabetes Mellitus; Drug Therapy, Combination; Female; Head; Hospitalization; Humans; Hypertension; Hypoglycemic Agents; Immunosuppressive Agents; Male; Metformin; Methotrexate; Middle Aged; Pemphigoid, Bullous; Prednisone; Retrospective Studies; Risk Factors; Tertiary Care Centers; Tetracycline; Treatment Failure

Inappropriate antibiotics prescription in dental healthcare delivery that may result in the emergence of antibiotic-resistant bacteria, is a worldwide concern. The objective of the study was to determine the antibiotics knowledge and prescription patterns among dentists in Nigeria.. A total of 160 questionnaires were distributed to dentists attending continuing education courses organized by two organizations in Southern and Northern parts of Nigeria. Data analysis was done using SPSS version 17.0.. A total of 146 questionnaires were returned, properly filled, out of 160 questionnaires, giving an overall response rate 91.3%. The clinical factors predominantly influenced the choice of therapeutic antibiotics among the respondents. In this study, the most commonly prescribed antibiotics among the respondents was a combination of amoxicillin and metronidazole. Of the respondents, 136 (93.2%) of them considered antibiotic resistance as a major problem in Nigeria and 102 (69.9%) have experienced antibiotics resistance in dental practice. The major reported conditions for prophylactic antibiotics among the respondents were diabetic mellitus, HIV/AIDS, history of rheumatic fever, other heart anomalies presenting with heart murmur and presence of prosthetic hip. The knowledge of adverse effects of antibiotics was greatest for tooth discoloration which is related to tetracycline.. Data from this study revealed the most commonly prescribed antibiotics as a combination of amoxicillin and metronidazole. There existed gaps in prophylactic antibiotic prescription, consideration in the choice of therapeutic antibiotics and knowledge of adverse effects of antibiotics among the studied dentists.

Topics: Acquired Immunodeficiency Syndrome; Amoxicillin; Anaphylaxis; Anti-Bacterial Agents; Antibiotic Prophylaxis; Attitude of Health Personnel; Dental Care; Dentists; Diabetes Mellitus; Drug Combinations; Drug Prescriptions; Drug Resistance, Bacterial; Education, Dental; Female; Heart Diseases; Hip Prosthesis; HIV Infections; Humans; Male; Metronidazole; Nigeria; Practice Patterns, Dentists'; Rheumatic Fever; Tetracycline; Tooth Discoloration

Humans with heterozygous loss-of-function mutations in the hepatocyte nuclear factor-1alpha (HNF1alpha) gene develop beta-cell-deficient diabetes (maturity-onset diabetes of the young type 3), indicating that HNF1alpha gene dosage is critical in beta-cells. However, whether increased HNF1alpha expression might be beneficial or deleterious for beta-cells is unknown. Furthermore, although it is clear that HNF1alpha is required for beta-cell function, it is not known whether this role is cell autonomous or whether there is a restricted developmental time frame for HNF1alpha to elicit gene activation in beta-cells. To address this, we generated a tetracycline-inducible mouse model that transcribes HNF1alpha selectively in beta-cells in either wild-type or Hnf1alpha-null backgrounds. Short-term induction of HNF1alpha in islets from adult Hnf1alpha(-/-) mice that did not express HNF1alpha throughout development resulted in the activation of target genes, indicating that HNF1alpha has beta-cell-autonomous functions that can be rescued postnatally. However, transgenic induction throughout development, which inevitably resulted in supraphysiological levels of HNF1alpha, strikingly caused a severe reduction of cellular proliferation, increased apoptosis, and consequently beta-cell depletion and diabetes. Thus, HNF1alpha is sensitive to both reduced and excessive concentrations in beta-cells. This finding illustrates the paramount importance of using the correct concentration of a beta-cell transcription factor in both gene therapy and artificial differentiation strategies.

Topics: Animals; Apoptosis; Cell Division; Cells, Cultured; Diabetes Mellitus; Fluorescent Antibody Technique; Gene Dosage; Gene Expression Regulation; Hepatocyte Nuclear Factor 1-alpha; Islets of Langerhans; Male; Mice; Mice, Knockout; Mice, Transgenic; Mutation; Reverse Transcriptase Polymerase Chain Reaction; Tetracycline; Transcription, Genetic; Transcriptional Activation

Topics: Adult; Aged; Anti-Bacterial Agents; Dental Plaque; Diabetes Mellitus; Erythromycin; Female; Humans; Hypoglycemic Agents; Insulin; Male; Microbial Sensitivity Tests; Middle Aged; Periodontal Pocket; Periodontitis; Staphylococcus; Tetracycline

Topics: Acidosis; Biguanides; Diabetes Mellitus; Drug Interactions; Humans; Phenformin; Tetracycline

Topics: Actinomyces; Actinomycosis; Aged; Anus Diseases; Colitis, Ulcerative; Crohn Disease; Diabetes Mellitus; Diagnosis, Differential; Female; Follow-Up Studies; Humans; Male; Middle Aged; Penicillin G; Rectal Diseases; Rectal Fistula; Suppuration; Tetracycline

Topics: Administration, Topical; Adult; Diabetes Complications; Diabetes Mellitus; Diabetic Coma; Foot Dermatoses; Gangrene; Humans; Hyperglycemia; Insulin; Ischemia; Skin Ulcer; Tetracycline; Tetracyclines

Topics: Carbimazole; Chlorpromazine; Diabetes Mellitus; Diazepam; Drug Prescriptions; Epilepsy; Female; Fetus; Heparin; Humans; Hyperthyroidism; Infant, Newborn; Insulin; Maternal-Fetal Exchange; Pregnancy; Pregnancy Complications; Pregnancy Trimester, First; Smoking; Streptomycin; Tetracycline; Warfarin

Topics: Anal Canal; Blood Glucose; Calcium; Candida; Carrier State; Cholesterol; Diabetes Mellitus; Erythromycin; Female; Humans; Iron; Male; Mouth; Prednisolone; Prednisone; Skin; Tetracycline; Vagina

Topics: Adult; Bone Development; Diabetes Mellitus; Humans; Microscopy, Fluorescence; Ribs; Tetracycline

Topics: Acinetobacter; Bacteremia; Chloramphenicol; Diabetes Mellitus; Drug Resistance; Drug Resistance, Microbial; Enterobacter; Escherichia coli; Fluorescent Antibody Technique; Kanamycin; Klebsiella; Liver Cirrhosis; Polymyxins; Proteus; Pseudomonas; Salmonella; Sepsis; Streptomycin; Tetracycline; Urinary Tract Infections

The mean cross-sectional area of Haversian systems in adult human ribs tends to be constant in the face of sevenfold changes in the rates at which these systems are made. This implies that different mechanisms control the total amount of cellular work in making Haversian systems and the rate at which this work is performed.

Topics: Adult; Biomedical Research; Bone and Bones; Diabetes Mellitus; Fluorescence; Haversian System; Humans; Protein Biosynthesis; Proteins; Ribs; Tetracycline

Topics: Abscess; Animals; Anti-Bacterial Agents; Bronchitis; Cross Infection; Diabetes Mellitus; Erythromycin; Furunculosis; Fusidic Acid; Gangrene; Humans; Leukemia; Lung Diseases; Penicillins; Pyelonephritis; Sepsis; Staphylococcal Infections; Streptomycin; Sulfonamides; Tetracycline

Topics: Alcaligenes; Anti-Bacterial Agents; Chloramphenicol; Colistin; Diabetes Mellitus; Enterobacteriaceae; Escherichia coli Infections; Female; Humans; Kanamycin; Klebsiella; Neomycin; Nitrofurantoin; Novobiocin; Penicillins; Polymyxins; Proteus Infections; Staphylococcal Infections; Streptomycin; Sulfonamides; Tetracycline; Urinary Tract Infections