tetracycline and Dentin--Secondary

Placement of mini-implants carries with it the risk of iatrogenic damage to the adjacent root surfaces. The aims of this study were to assess the type of trauma incurred on tooth roots after contact or approximation with mini-implants during placement, and to observe and analyze the healing responses via histologic analysis.. Four male minipigs were used as experimental subjects. Twenty mini-implants (1.6 × 8 mm) were implanted in each minipig into the buccal sides of all 4 quadrants between the roots of teeth so that contact or approximation between the mini-implant and root surface occurred, with the aid of dental fluoroscopy. All mini-implants on the left side of the mouth were left in situ, and all mini-implants on the right side were removed immediately after placement. The minipigs were killed at 4-week intervals up to week 16, and histologic sections were made.. When mini-implants were left in situ, the root surface was mostly resorbed away from the mini-implant thread. Partial repair started at 8 weeks. When the mini-implant thread was left touching the root, there was no normal healing response. If the mini-implant was placed less than 1 mm from the periodontal ligament, resorption was evident on the root surface. Abnormal healing responses were seen when the pulp tissue was ruptured, mostly through osteodentin formation. In all instances after mini-implant removal immediately after placement, varying degrees of cementum repair was observed.. Immediate removal of the mini-implant leads to cementum repair, whereas leaving the mini-implant in place will cause either a delay in repair or no repair. Placing mini-implants less than 1 mm from the root surface causes root-surface resorption.

Topics: Animals; Anthraquinones; Cementogenesis; Dental Cementum; Dental Implants; Dental Pulp; Dentin, Secondary; Fluoresceins; Fluorescent Dyes; Fluoroscopy; Male; Orthodontic Anchorage Procedures; Periodontal Ligament; Root Resorption; Specific Pathogen-Free Organisms; Swine; Swine, Miniature; Tetracycline; Time Factors; Tooth Root; Wound Healing

Topics: Adult; Dental Devices, Home Care; Dental Pulp Cavity; Dentin, Secondary; Female; Humans; Tetracycline; Tooth Bleaching; Tooth Discoloration

Topics: Animals; Dental Caries; Dental Enamel; Dental Fissures; Dentin; Dentin, Secondary; Female; Male; Microscopy, Fluorescence; Molar; Odontoblasts; Rats; Rats, Inbred Strains; Staining and Labeling; Tetracycline

The rate of tertiary dentin produced in response to four lining materials placed in standardized cavities in dogs' teeth was measured over a period of 119 days. Sterile denatured dentin ash was used as a control material in an attempt to quantify tertiary dentin formation in response to cavity preparation. In all periods the amounts of tertiary dentin formed beneath the control material were significantly smaller than those formed beneath any of the four lining materials. In the first 98 days the rate of formation of tertiary dentin beneath calcium hydroxide (Calxyl) was significantly greater than that beneath the material containing corticosteroid (Ledermix cement). In the last 21 days there was no difference in daily rates of tertiary dentin production beneath all of the lining materials. With all the lining materials there was a highly significant difference between the daily amounts of tertiary dentin formed in the first 6 weeks and those in the last 11 weeks.

Topics: Animals; Calcium Hydroxide; Demeclocycline; Dental Cavity Lining; Dental Cavity Preparation; Dentin; Dentin, Secondary; Dogs; Drug Combinations; Staining and Labeling; Tetracycline; Time Factors; Triamcinolone Acetonide; Zinc Oxide-Eugenol Cement

The teeth of dogs in which the root dentin was incompletely formed were prepared so that dentin was exposed. Tetracycline was then injected and the teeth were studied for dentin formation after the periods of 18, 20, 48 and 76 weeks. After the dentin was exposed slightly, reparative secondary dentin was not formed in the pulp horn area; However, a considerable amount of dentin was formed at the walls of the pulp chambers and the root canals. No changes were noted in the pulp horn area in the prepared teeth in comparison with unprepared teeth. The results imply that dentinal changes in the pulp horn area caused by dentinal cutting are more closely related to the depth of dentinal preparation than to the age of the tooth and to the elapsed time after preparation.

Topics: Animals; Dentin; Dentin, Secondary; Dogs; Tetracycline; Tooth Root

The influence of formocresol treatment on the pulp tissue of 24 primary monkey teeth was studied using tetracycline and 3H-proline labeling techniques. Six untreated monkey teeth served as controls. The tetracycline labeled teeth were examined between 352 and 600 d, following treatment. The 3H-proline labeled teeth were observed over a period of 22-607 d, the isotope being administered 15 d before extraction. The labeling was evaluated in the coronal, middle and apical area of the roots. Dentin formation as indicated by tetracycline labeling was observed in both control teeth and successfully treated teeth, as well as in some of the unsuccessfully treated teeth. The average dentin formation rate per day varied from 1 micrometer in the control teeth to 0.14 micrometer in the pulpotomized teeth (P less than 0.01). Success of treatment was of significant importance for the amount of dentin formation (P less than 0.001). Labeling with 3H-proline, indicating collagen synthesis, could be observed in the pulp and predentin of the majority of areas judged to be normal, degenerated or inflamed. Labeling was not observed in fixed or necrotic tissue. In degenerated pulp tissue the proline labeling was clearly reduced. The findings indicate that dentin formation and collagen synthesis may take place in formaldehyde influenced pulp tissue although at a decreased rate.

Topics: Animals; Collagen; Dental Pulp; Dentin, Secondary; Formocresols; Haplorhini; Proline; Pulpotomy; Tetracycline; Tooth, Deciduous; Tritium

Topics: Animals; Dental Cavity Preparation; Dental Cements; Dental Materials; Dental Pulp Capping; Dentin, Secondary; Dogs; Gutta-Percha; Microscopy, Fluorescence; Tetracycline; Zinc Oxide-Eugenol Cement

Topics: Animals; Dental Caries; Dental Caries Susceptibility; Dentin, Secondary; Rats; Tetracycline; Tooth Discoloration

Topics: Animals; Calcium Hydroxide; Dental Pulp Capping; Dentin; Dentin, Secondary; Dentinogenesis; Dogs; Microscopy, Fluorescence; Phenytoin; Tetracycline

Topics: Animals; Autoradiography; Dental Pulp Capping; Dentin, Secondary; Microscopy, Fluorescence; Molar; Proline; Rats; Tetracycline; Tritium

Topics: Animals; Dentin, Secondary; Microradiography; Microscopy, Fluorescence; Proline; Rats; Tetracycline; Tritium

Topics: Animals; Dentin; Dentin, Secondary; Fluorescence; Guinea Pigs; Histocytochemistry; Microradiography; Microscopy; Microscopy, Fluorescence; Pharmacology; Research; Tetracycline; Tooth Calcification

Topics: Alloxan; Animals; Dentin; Dentin, Secondary; Diabetes Mellitus, Experimental; Protein Synthesis Inhibitors; Rats; Research; Tetracycline

Topics: Absorptiometry, Photon; Acrylic Resins; Adolescent; Adult; Aged; Animals; Child; Dental Cementum; Dentin; Dentin, Secondary; Female; Haplorhini; Humans; In Vitro Techniques; Male; Mandible; Microradiography; Microscopy, Fluorescence; Middle Aged; Tetracycline; Tooth Abrasion