tetracycline and Dental-Caries

Topics: Anti-Bacterial Agents; Dental Caries; Female; Gastrointestinal Tract; Humans; Mouth; Pregnancy; Rats; Research; Tetracycline

Topics: Bone and Bones; Chemical Phenomena; Chemistry; Collagen; Dental Caries; Dental Caries Susceptibility; Dental Enamel Hypoplasia; Dental Pulp; Dentin; Fluorescence; Growth; Humans; Neoplasms; Polysaccharides; Tetracycline; Tetracyclines; Tooth Discoloration

The aim of the present study is to evaluate the probiotic effect of. Plaque samples from permanent first molars were collected and transferred aseptically onto Mitis-Salivarius agar and incubated at 37°C for 24 hours in the presence of 5-10% CO. Mutans streptococci exhibited positive inhibition with both the probiotic strains and. Dental caries remains silent epidemic and increasing antibiotic resistance is another major challenge that threatens the world. Newer methods such as whole-bacteria replacement therapy using probiotics for decreasing harmful oral pathogens and reducing the intake of antibiotics must be explored. More researches to promote use of probiotics should be initiated due to its possible preventive and health maintenance benefits providing an end to new cavities and antibiotic resistance.

Topics: Agar; Anti-Bacterial Agents; Cephalothin; Dental Caries; Erythromycin; Humans; Lactobacillus; Lactobacillus acidophilus; Penicillins; Probiotics; Streptococcus mutans; Tetracycline; Vancomycin

Dental caries remains the most prevalent oral infectious disease worldwide. In this study, the antibacterial and the antibiofilm activities of five essential oils (EO's): eugenol (EUG), carvacrol (CAR), thymol (TYH), p-cymene (CYM) and γ-terpinene (TER) were tested (alone or in combinaison with tetracycline) against oral bacteria. In addition, their potential roles to enhance the accumulation of ethidium bromide (EtBr) in bacterial cells were tested. Our results indicated that EO's induced a selective antimicrobial activity. A synergistic effect of EO's and tetracycline (TET) was noticed with a reduction rate ranged from 2 to 8-fold. In addition, the efflux of EtBr was inhibited with a decrease in loss of EtBr from the bacteria. On the other hand a significant anti-biofilm activities of EO's (alone or combined with antibiotics) was noticed. In conclusion the tested EO's may be considered as a potential natural source with a resistance-modifying activity and may be applied to eradicate bacterial biofilm.

Topics: Anti-Bacterial Agents; Bacteria; Biofilms; Cyclohexane Monoterpenes; Cymenes; Dental Caries; Dental Enamel; Drug Resistance, Bacterial; Drug Synergism; Ethidium; Eugenol; Microbial Sensitivity Tests; Microbiota; Microscopy, Electron, Scanning; Monoterpenes; Mouth; Oils, Volatile; Staphylococcus aureus; Tetracycline; Thymol

The study was carried out to see the diversity of oral microflora and its antibiotic sensitivity test in children of age group 6 to 12 years was carried.. Total 50 patients of age group 6 to 12 years were analyzed for their oral microflora and then checked for the antibiotic susceptibility test. The samples that were collected were incubated at 37°C for 48 hours. Once dispersed samples were taken and Gram staining was done, also they were spread on to a number of freshly prepared agar plates and incubated to allow cells to form microbial colony.. The result showed microflora common in all types, Gram-positive facultative anaerobic rods and cocci. In normal children Gram-positive facultative anaerobic and fermenting cocci were predominant where as in children with caries growth of microbiota that were Gram-negative and positive, capnophilic, motile and anaerobic rods and cocci belonging to members of genera S. mutans and A. actinomycetemcomitans was seen.. By the present study it has been concluded that the number of bacteria determined by microscopic counts was twice as high in caries patients as in healthy sites, and also recommended that amoxicillin, ampicillin and amikacin are the most effective antibacterial drugs for the treatment of dental caries.

Topics: Aggregatibacter actinomycetemcomitans; Amikacin; Amoxicillin; Ampicillin; Anti-Bacterial Agents; Bacteria; Bacterial Load; Bacteriological Techniques; Child; Clindamycin; Dental Caries; Enterococcus faecalis; Erythromycin; Gram-Negative Bacteria; Gram-Positive Bacteria; Humans; Microbial Sensitivity Tests; Mouth; Porphyromonas gingivalis; Streptococcus mutans; Tetracycline; Vancomycin

Antimicrobial peptides (AMPs) are naturally occurring, broad-spectrum antimicrobial agents that have recently been examined for their utility as therapeutic antibiotics. Unfortunately, they are expensive to produce and are often sensitive to protease digestion. To address this problem, we have examined the activity of a peptide mimetic whose design was based on the structure of magainin, exhibiting its amphiphilic structure. We demonstrate that this compound, meta-phenylene ethynylene (mPE), exhibits antimicrobial activity at nanomolar concentrations against a variety of bacterial and Candida species found in oral infections. Since Streptococcus mutans, an etiological agent of dental caries, colonizes the tooth surface and forms a biofilm, we quantified the activity of this compound against S. mutans growing under conditions that favor biofilm formation. Our results indicate that mPE can prevent the formation of a biofilm at nanomolar concentrations. Incubation with 5 nM mPE prevents further growth of the biofilm, and 100 nM mPE reduces viable bacteria in the biofilm by 3 logs. Structure-function analyses suggest that mPE inhibits the bioactivity of lipopolysaccharide and binds DNA at equimolar ratios, suggesting that it may act both as a membrane-active molecule, similar to magainin, and as an intracellular antibiotic, similar to other AMPs. We conclude that mPE and similar molecules display great potential for development as therapeutic antimicrobials.

Topics: Actinomyces viscosus; Alkynes; Anti-Infective Agents; Bacteria; Bacterial Proteins; Biofilms; Candida; Dental Caries; DNA; Escherichia coli; Humans; Kinetics; Lipopolysaccharides; Magainins; Microbial Sensitivity Tests; Molecular Structure; Mouth; Peptides; Phenethylamines; Protein Binding; Staphylococcus aureus; Streptococcus mutans

The objectives of the study were to describe the oral health status and treatment needs of the 5- to 6-year-old and 12-year-old children in Southern China; to describe the patterns of oral health behaviors, knowledge, and attitudes among the 12-year-olds; and to assess the effects of socio-behavioral factors on the 12-year-old children's dental caries experiences. The study sample was comprised of 1,587 5- to 6-year-old and 1,576 12-year-old urban and rural schoolchildren living in Guangdong Province. Three calibrated dentists clinically examined the children, and trained interviewers interviewed the 12-year-olds. Caries prevalence of the 5- to 6-year-old children was high (urban 78% vs. rural 86%); the mean dmft of the urban and rural children was 4.8 and 7.0, respectively. The caries prevalence and mean DMFT score of the 12-year-olds were 41% and 0.9 (urban) and 42% and 0.9 (rural). Only 2% of the 12-year-olds exhibited no calculus or gingival bleeding, while more than 70% had calculus. In conclusion, there is an urgent need for establishing caries-preventive activities for preschool children. The prevalence of caries among the 12-year-olds was not high, but their periodontal condition was unsatisfactory. Knowledge about gum bleeding and the use of fluoride was low. More oral health education activities should be organized, especially for the rural children.

Topics: Analysis of Variance; Chi-Square Distribution; Child; Child Behavior; Child, Preschool; China; Dental Caries; DMF Index; Female; Health Behavior; Health Education, Dental; Health Knowledge, Attitudes, Practice; Humans; Male; Oral Health; Prevalence; Rural Health; Surveys and Questionnaires; Tetracycline; Tooth Discoloration; Urban Health

Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real-time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low-level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image-processing systems.

Topics: Animals; Bone and Bones; Bone Development; Bone Transplantation; Dental Caries; Dental Materials; Dental Restoration, Permanent; Fluorescent Antibody Technique; Humans; Immunohistochemistry; Microscopy, Fluorescence; Osteoclasts; Rabbits; Rats; Rhodamines; Tetracycline

Topics: Animals; Dental Caries; Dental Enamel; Dental Fissures; Dentin; Dentin, Secondary; Female; Male; Microscopy, Fluorescence; Molar; Odontoblasts; Rats; Rats, Inbred Strains; Staining and Labeling; Tetracycline

Many effective methods currently are available for preventing oral diseases and promoting oral health. The responsibility for ensuring the optimal use of these approaches is shared by members of the dental profession and other primary care providers in the present health care delivery system. Recognition of each provider's role and greater collaborative efforts could enhance the gains that already have been made with respect to improving the oral health status of children and adolescents.

Topics: Child; Child, Preschool; Dental Caries; Diet; Fluoridation; Fluorides; Fluorides, Topical; Gingivitis; Humans; Infant; Oral Health; Tetracycline; Tobacco Use Disorder; Tooth Discoloration; Tooth Diseases

Complaints of enamel defects in American Indian children residing on the St. Regis reservation in New York State prompted an epidemiological study. The results of that study, reported earlier (Rebich et al., 1983), indicated that over one-fifth of the American Indian children had discoloration of the dentition due to ingestion of tetracycline during the years of tooth formation. These data also provided an ideal opportunity to examine the link between tetracycline staining and caries which has been postulated by previous authors. American Indian children, ages 7-18, were found to have a higher caries experience than other children and a lower rate of dental service utilization, as evidenced by the filled component of the DMFS index (FS/DMFS). Within the American Indian population, however, no indication was found of any association between tetracycline staining and dental caries.

Topics: Adolescent; Age Factors; Child; Dental Caries; DMF Index; Humans; Indians, North American; New York; Tetracycline; Tooth Discoloration

Topics: Child; Dental Caries; Dental Enamel; Fluoridation; Fluorides; Fluorosis, Dental; Humans; Tetracycline; Tooth Discoloration

Topics: Dental Caries; Humans; Tetracycline; Time Factors; Tooth; Tooth Discoloration

The prevalence, distribution, and interrelationship between tetracycline discoloration, enamel defects, and dental caries was determined in 86 patients with cystic fibrosis (age 3 to 24 years). The dental caries experience was compared to control subjects matched for sex, race, exposure to optimally fluoridated water, chronologic age, and dental age. The findings indicate a high prevalence of tetracycline discolorations and enamel defects but a significantly reduced dental caries experience in these patients. However, interrelationships between these three factors were not strikingly evident. The presence of an altered oral health status in cystic fibrosis patients and the increased prevalence of this disease due to recent therapeutic and diagnostic advances in its management suggest the need for further familiarization with the dental manifestations of cystic fibrosis.

Topics: Adolescent; Adult; Child; Child, Preschool; Cystic Fibrosis; Dental Caries; Dental Caries Susceptibility; Dental Enamel; Dental Enamel Hypoplasia; Female; Humans; Male; Tetracycline; Tooth Discoloration

Topics: Amino Acids; Dental Caries; Dental Pulp Devitalization; Dentin; Fluorescence; Humans; Spectrometry, Fluorescence; Tetracycline; Tissue Extracts

Topics: Ameloblasts; Animals; Dental Caries; Dental Enamel; Microscopy, Electron; Rats; Tetracycline

Streptococcus faecalis strains ND539 and OG1 have been previously shown to be cariogenic in gnotobiotic animals. Deoxyribonucleic acid analyses have revealed the presence of a single 26-megadalton plasmid designated pAM539 in the former strain, whereas the latter strain was found to be plasmid-free. By gene transfer experiments, it was possible to construct isogenic pairs of strains that differed only with regard to the presence or absence of pAM539. Comparative studies of isogenic pairs showed that the presence of pAM539 conferred bacterial sensitivity to a bacteriocin produced by S. faecalis strain 5952.

Topics: Animals; Bacteriocins; Conjugation, Genetic; Dental Caries; DNA, Bacterial; Enterococcus faecalis; Plasmids; Rats; Streptomycin; Tetracycline

The influence of tetracyclines embedded in hard substances on caries predisposition of the teeth is tested in Syrian hamsters. Caries predisposition in the teeth that mineralized under tetracycline influence is by 67% smaller than in the teeth of the animals of the control group. This phenomenon can neither be explained by a permanent change in the microflora of the oral cavity nor by a local effect of the antibiotic resulting from dissolving processes when it is released. The reduction in caries predisposition can only be due to a change in the mineral structure of the enamel that developed under the influence of tetracycline.

Topics: Animals; Cricetinae; Dental Caries; Dental Caries Susceptibility; Dentinogenesis; Female; Molar; Pregnancy; Pregnancy, Animal; Tetracycline

Topics: Adolescent; Blood Protein Disorders; Child; Child, Preschool; Dental Caries; DMF Index; Female; Gingivitis; Gingivitis, Necrotizing Ulcerative; Humans; IgA Deficiency; Male; Mouth Breathing; Oral Hygiene; Periodontal Diseases; Tetracycline; Tooth Discoloration

Topics: Acetaminophen; Adult; Brompheniramine; Dental Caries; Edema; Female; Humans; Iatrogenic Disease; Oral Hemorrhage; Periapical Tissue; Periodontal Diseases; Pulpectomy; Root Canal Obturation; Root Canal Therapy; Sodium Hypochlorite; Tetracycline; Therapeutic Irrigation

Topics: Actinomyces; Actinomycosis; Adult; Biopsy; Bronchial Neoplasms; Cephalosporins; Dental Caries; Epilepsy; Female; Humans; Lung Diseases; Male; Middle Aged; Periostitis; Radiography; Tetracycline

Topics: Child, Preschool; Dental Caries; Dental Enamel Hypoplasia; Fluoridation; Humans; Infant; Infant, Newborn; Tetracycline; Tooth; Tooth Diseases; Tooth Eruption; Tooth, Supernumerary

Topics: Calcitonin; Calcium; Child; Child, Preschool; Dental Caries; Dentition; Fluorides; Humans; Infant; Infant, Newborn; Odontogenesis; Parathyroid Hormone; Pediatric Dentistry; Phosphorus; Tetracycline; Tooth Abnormalities; Tooth Calcification; Tooth, Deciduous; Vitamin D

Actinobolin, a known inhibitor of protein synthesis, has been shown not to interfere selectively with acid production or dextransucrase activity in a cariogenic streptococcus when the antibiotic is added to a concentration of 500 mug/ml. It has also been shown that actinobolin does not alter the total in vivo flora of the oral cavity of the rat when tested in a rat caries model system. A culture of cariogenic streptococci, adapted to in vitro growth in the presence of 1 mg of actinobolin per ml, has also been isolated.

Topics: Animals; Anti-Bacterial Agents; Bacteriological Techniques; Culture Media; Dental Caries; Dextrans; Diet; Disease Models, Animal; Drug Resistance, Microbial; Genetics, Microbial; Glucosyltransferases; Hydrogen-Ion Concentration; Male; Molar; Mutation; Rats; Rats, Inbred Strains; Streptococcus; Tetracycline; Time Factors

Topics: Age Factors; Child, Preschool; Dental Caries; Female; Gestational Age; Humans; Infant; Male; Minerals; Oxytetracycline; Pregnancy; Rolitetracycline; Tetracycline; Tooth Abnormalities; Tooth Calcification; Tooth Discoloration; Tooth, Deciduous

Topics: Dental Calculus; Dental Caries; Dental Deposits; Dental Plaque; Diagnosis, Oral; Fluoresceins; Humans; Tetracycline; Tooth Discoloration; Ultraviolet Rays

Topics: Adult; Arteritis; Dental Caries; Dexamethasone; Fluorescent Dyes; Gingivitis; Humans; Hypersensitivity; Male; Phlebitis; Photography; Radiography; Remission, Spontaneous; Retinal Vessels; Rhinitis; Streptomycin; Tetracycline; Tonsillitis

Topics: Child; Dental Caries; Female; Humans; Infant, Newborn; Odontogenesis; Pregnancy; Tetracycline; Tooth Abnormalities; Tooth Calcification; Tooth Discoloration

Topics: Child, Preschool; Dental Caries; Dental Caries Susceptibility; Dental Enamel; Hardness; Humans; Tetracycline; Time Factors; Tooth, Deciduous

Topics: Animals; Dental Caries; Dental Caries Susceptibility; Dentin, Secondary; Rats; Tetracycline; Tooth Discoloration

Topics: Adolescent; Age Factors; Child; Child, Preschool; Chlortetracycline; Dental Caries; Germany, West; Humans; Infant; Oxytetracycline; Tetracycline; Tooth; Tooth Abnormalities; Tooth Discoloration; Tooth, Deciduous

Topics: Child; Dental Caries; Dental Enamel Hypoplasia; Dental Pulp Necrosis; Dentinogenesis Imperfecta; Female; Humans; Infant, Newborn; Jaundice, Neonatal; Porphyrias; Pregnancy; Pregnancy Complications; Tetracycline; Tooth Discoloration

Topics: Animals; Autoradiography; Calcium; Calcium Isotopes; Carbon Isotopes; Dental Caries; Dental Enamel; Histocytochemistry; Microradiography; Microscopy, Fluorescence; Proline; Rats; Tetracycline

Topics: Child; Child, Preschool; Dental Caries; Fluorescence; Humans; Infant; Infant, Newborn; Mass Screening; Tetracycline; Tooth Discoloration; Tooth, Deciduous; Ultraviolet Rays

Topics: Animals; Autoradiography; Calcification, Physiologic; Calcium Isotopes; Carbon Isotopes; Dental Caries; Dental Enamel; Dental Enamel Proteins; Microradiography; Proline; Rats; Tetracycline

Topics: Carbohydrates; Child; Child, Preschool; Clubfoot; Dental Caries; Erythema; Extremities; Female; Humans; Male; Periodontitis; Tetracycline; Tooth Discoloration

Topics: Adolescent; Child; Child, Preschool; Chlortetracycline; Cystic Fibrosis; Dental Caries; Female; Fluorescence; Humans; Incisor; Male; Oxytetracycline; Tetracycline; Tooth Calcification; Tooth Discoloration

Topics: Animals; Cariogenic Agents; Dental Caries; Dental Caries Susceptibility; Dental Plaque; Dietary Carbohydrates; Erythromycin; Male; Mouth; Rats; Tetracycline

Topics: Animal Feed; Animals; Cariogenic Agents; Dental Caries; Dental Caries Susceptibility; Disease Reservoirs; Food; Rats; Tetracycline

Topics: Adolescent; Adult; Child; Dental Caries; DMF Index; Female; Humans; Male; Models, Theoretical; Penicillins; Respiratory Tract Diseases; Rheumatic Fever; Tetracycline

Topics: Animals; Cricetinae; Dental Caries; Dental Caries Susceptibility; Fluorides, Topical; Penicillins; Tetracycline

Topics: Abnormalities, Drug-Induced; Anesthesia, Dental; Anesthetics; Dental Caries; Dentistry; Female; Fetal Death; Fetal Diseases; Fluorides; Gingivitis; Humans; Hyperemesis Gravidarum; Hypotension; Periodontal Diseases; Pregnancy; Pregnancy Complications; Radiography, Dental; Stress, Physiological; Tetracycline

Topics: Abnormalities, Drug-Induced; Anti-Bacterial Agents; Child; Demeclocycline; Dental Calculus; Dental Caries; Erythromycin; Female; Fluorescence; Humans; Infant; Oxytetracycline; Pregnancy; Tetracycline; Tetracyclines; Tooth, Deciduous; Toxicology

Topics: Animals; Anti-Bacterial Agents; Dental Caries; Female; Humans; Postpartum Period; Pregnancy; Protein Synthesis Inhibitors; Rats; Tetracycline