tetracycline and Corynebacterium-Infections

Although the clinical presentation of Hidradenitis Suppurativa (HS) is strongly reminiscent of bacterial infection, the role of bacteria remains controversial. Studies have isolated an array of different bacterial specimens as well as biofilm formation in lesional HS skin. Consistent findings of Gram-positive cocci and -rods including Staphylococus aureus, Coagulase-negative staphylococci (CoNS) and Corynebacterium species (spp) in deep tissue samples have been demonstrated in HS. Although efficacy of antibiotics, i.e., rifampicin, clindamycin or tetracycline may support a microbial role in disease pathogenesis, the most often isolated bacterial specimens are commensal bacteria (CoNS).

Topics: Anti-Bacterial Agents; Biofilms; Clindamycin; Corynebacterium Infections; Hidradenitis Suppurativa; Humans; Rifampin; Skin; Staphylococcal Skin Infections; Staphylococcus aureus; Staphylococcus epidermidis; Tetracycline

Topics: Acne Vulgaris; Administration, Oral; Administration, Topical; Clindamycin; Corynebacterium Infections; Double-Blind Method; Female; Humans; Male; Microbial Sensitivity Tests; Random Allocation; Tetracycline

The tetracycline resistance region of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium striatum M82B was analyzed in Corynebacterium glutamicum ATCC 13032 and confined to a 4.4-kb SphI-Sa/I DNA fragment. Nucleotide sequence analysis revealed two open reading frames, termed tetA and tetB, specifying proteins of 513 and 528 amino acids, respectively. The deduced amino acid sequences of tetAB displayed similarity to ATP-binding cassette transporters including StrV and StrW of Streptomyces glaucescens which are proposed to play a role in the export of streptomycin-like aminoglycosides. An antibiotic susceptibility screening in C. glutamicum showed that the tetAB genes confer resistance to tetracycline, oxytetracycline and to the structurally and functionally unrelated beta-lactam antibiotic oxacillin.

Topics: Amino Acid Sequence; Anti-Bacterial Agents; Antiporters; ATP-Binding Cassette Transporters; Bacterial Proteins; Corynebacterium; Corynebacterium Infections; Drug Resistance, Multiple; Genes, Bacterial; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Oxacillin; Oxytetracycline; Penicillin Resistance; Penicillins; R Factors; Sequence Alignment; Sequence Analysis, DNA; Tetracycline; Tetracycline Resistance

The comparative study of 44 isolates of Corynebacterium group D2, from urine, most frequently, shows the pathogenic role of these bacteria in urinary tract infection, with or without urinary stones. These microorganisms have an opportunistic behaviour in other non-urinary sites, and become pathogen in immunosuppressed conditions. The rapid tests as urease, glucose acidification, nitrate reductase, associated with multiple resistance to antibiotics (beta-lactams and aminosides) identify easily Corynebacterium group D2, from 48 h cultures under CO2 conditions. The results of MIC determination of 10 antibiotics, show the high activity (100% sensitivity) of vancomycin and pristinamycin, with MIC modes, respectively, 0.5 and 0.03 mg/l. These antibiotics are the most useful for the treatment of non-urinary infections. Among quinolones, the most active agents are ciprofloxacin and ofloxacin (MIC modes: 4 and 2 mg/l), so these antimicrobials could be used for the treatment of urinary tract infections caused by Corynebacterium group D2.

Topics: Anti-Bacterial Agents; Ciprofloxacin; Corynebacterium; Corynebacterium Infections; Drug Resistance, Microbial; Enoxacin; Female; Humans; Male; Naphthyridines; Norfloxacin; Ofloxacin; Oxazines; Peptides, Cyclic; Pipemidic Acid; Rifampin; Tetracycline; Tetracycline Resistance; Urinary Tract Infections; Vancomycin; Virginiamycin

Topics: Carrier State; Corynebacterium; Corynebacterium Infections; Female; Humans; Male; Tetracycline; Ureaplasma; Urethritis

Topical antibiotics were used on patients with acne vulgaris. Corynebacterium acnes organisms from open comedones were quantitated during treatment, and the progress of the disease was evaluated. Clindamycin lotion completely suppressed the growth of C acnes organisms, whereas erythromycin and tetracycline did not depress the C acnes counts. Taken as a group, these antibiotics gave a substantial improvement of the disease on the treated side as compared with paired untreated sides of the face and back.

Topics: Acne Vulgaris; Administration, Topical; Anti-Bacterial Agents; Clindamycin; Corynebacterium Infections; Drug Therapy, Combination; Erythromycin; Erythromycin Estolate; Humans; Microbial Sensitivity Tests; Propionibacterium acnes; Skin; Tetracycline

A patient with nonspecific urethritis (NSU) and his consort were examined for infection with NSU corynebacteria, mycoplasmas, and gonococci. No classic and T-mycoplasmas or gonococci were cultured, but one species of NSU corynebacteria was isolated not only from the patient's urethral discharge during three episodes of NSU but also from his consort. It was not isolated after successful treatment of the patient with tetracycline and the use of condoms prevented reoccurrence of urethritis. This NSU corynebacterium was isolated previously from one epididymitis patient and two NSU patients but not from any of the normal male and female subjects examined. Therefore, this strain is considered to be one of the etiologic agents of NSU and female subjects are asymptomatic carriers. In consequence, it is suggested that NSU corynebacteria which are commensals and pathogens of the male and female urogenital tracts should be incorporated in a new species, of the Coryneform group, and that this strain should be the type species, Corynebacterium genitalium n. sp.

Topics: Adult; Contraceptive Devices, Male; Corynebacterium; Corynebacterium Infections; Female; Humans; Male; Sexually Transmitted Diseases; Tetracycline; Urethritis

Topics: Acne Vulgaris; Administration, Topical; Adolescent; Benzoyl Peroxide; Corynebacterium Infections; Drug Therapy, Combination; Fatty Acids; Fatty Acids, Nonesterified; Humans; Peroxides; Propionibacterium acnes; Skin; Tetracycline; Tretinoin; Vitamin A

Topics: Ampicillin; Animals; Brucellosis; Cell Count; Complement Fixation Tests; Corynebacterium Infections; Disease Models, Animal; Drug Combinations; Drug Interactions; Evaluation Studies as Topic; Female; Hemagglutination Tests; Injections, Intraperitoneal; Mice; Organ Size; Propionibacterium acnes; Pyrimidines; Spleen; Sulfamethoxazole; Tetracycline; Trimethoprim