tetracycline and Carcinoma--Squamous-Cell

In a pilot study, we showed that topical administration of a tetracycline could decrease oral bacteria levels for 6 hours in patients who underwent oral cancer surgery combined with tracheotomy and flap reconstruction. This multicenter, randomized control trial aimed to investigate the effectiveness of topical application of tetracycline ointment for prevention of surgical site infection (SSI) associated with major oral cancer surgery.. One hundred seventeen patients who underwent oral cancer resection combined with neck dissection, flap reconstruction, and tracheotomy were divided randomly into an intervention group (n = 56) and a control group (n = 61). The intervention consisted of topical administration of tetracycline ointment on the dorsum of the tongue every 6 hours for 48 hours postoperatively. Factors relating to the occurrence of SSI in both groups were subjected to logistic regression analysis.. SSI occurred in 11 patients (19.6%) in the intervention group and 22 patients (36.1%) in the control group. Multivariate analysis showed that a longer operating time and not receiving topical tetracycline were independent risk factors for development of SSI.. Administration of topical tetracycline for 48 hours postoperatively is an effective way of preventing SSI after oral cancer surgery.

Topics: Administration, Topical; Aged; Anti-Bacterial Agents; Carcinoma, Squamous Cell; Female; Humans; Male; Mouth Neoplasms; Pilot Projects; Surgical Flaps; Surgical Wound Infection; Tetracycline; Treatment Outcome

Topics: Carcinoma, Squamous Cell; Humans; Lung Neoplasms; Methotrexate; Tetracycline; Time Factors

Tetracycline is a photosensitising medication that increases skin vulnerability to UV-related damage.. We prospectively examined tetracycline use and risk of incident melanoma, squamous cell carcinoma (SCC), and basal cell carcinoma (BCC) based on 213 536 participants from the Nurses' Health Study (NHS), NHS2, and Health Professionals Follow-up Study. Information on ever use of tetracycline was asked via questionnaire. Diagnoses of melanoma and SCC were pathologically confirmed.. Tetracycline use was associated with a modestly increased risk of BCC, but was not associated with melanoma or SCC.

Topics: Adult; Carcinoma, Basal Cell; Carcinoma, Squamous Cell; Female; Humans; Incidence; Male; Melanoma; Middle Aged; Photosensitizing Agents; Prospective Studies; Risk; Skin Neoplasms; Tetracycline; United Kingdom

Esophageal cancer occurs as either squamous cell carcinoma (ESCC) or adenocarcinoma. ESCCs comprise almost 90% of cases worldwide, and recur with a less than 15% five-year survival rate despite available treatments. The identification of new ESCC drivers and therapeutic targets is critical for improving outcomes. Here we report that expression of the human DEK oncogene is strongly upregulated in esophageal SCC based on data in the cancer genome atlas (TCGA). DEK is a chromatin-associated protein with important roles in several nuclear processes including gene transcription, epigenetics, and DNA repair. Our previous data have utilized a murine knockout model to demonstrate that Dek expression is required for oral and esophageal SCC growth. Also, DEK overexpression in human keratinocytes, the cell of origin for SCC, was sufficient to cause hyperplasia in 3D organotypic raft cultures that mimic human skin, thus linking high DEK expression in keratinocytes to oncogenic phenotypes. However, the role of DEK over-expression in ESCC development remains unknown in human cells or genetic mouse models. To define the consequences of Dek overexpression in vivo, we generated and validated a tetracycline responsive Dek transgenic mouse model referred to as Bi-L-Dek. Dek overexpression was induced in the basal keratinocytes of stratified squamous epithelium by crossing Bi-L-Dek mice to keratin 5 tetracycline transactivator (K5-tTA) mice. Conditional transgene expression was validated in the resulting Bi-L-Dek_K5-tTA mice and was suppressed with doxycycline treatment in the tetracycline-off system. The mice were subjected to an established HNSCC and esophageal carcinogenesis protocol using the chemical carcinogen 4-nitroquinoline 1-oxide (4NQO). Dek overexpression stimulated gross esophageal tumor development, when compared to doxycycline treated control mice. Furthermore, high Dek expression caused a trend toward esophageal hyperplasia in 4NQO treated mice. Taken together, these data demonstrate that Dek overexpression in the cell of origin for SCC is sufficient to promote esophageal SCC development in vivo.

Topics: 4-Nitroquinoline-1-oxide; Animals; Carcinoma, Squamous Cell; DNA-Binding Proteins; Epithelium; Esophageal Neoplasms; Esophageal Squamous Cell Carcinoma; Gene Expression Regulation, Neoplastic; Keratinocytes; Mice, Transgenic; Oncogene Proteins; Poly-ADP-Ribose Binding Proteins; Response Elements; Tetracycline; Tongue; Transgenes

Alloxan-induced diabetic rats showed proliferative changes in the forestomach, accompanied by chronic inflammation, and one lesion progress to squamous cell carcinoma (SCC) without distant metastasis. The authors demonstrated that these lesions might be caused by Candida albicans infection. Antimicrobial therapy, particularly tetracycline treatment, has been blamed for a reduction in the number of competing bacterial organisms, which is frequently mentioned as a cause of candidiasis. The objective of this study is to ascertain whether or not tetracycline treatment can accelerate early-onset of C. albicans infection and the proliferative changes in this diabetic model. Alloxan-induced diabetic rats were given chlorinated water (AL group) and tetracycline solution (0.1% during week 1 and 0.01% thereafter) as drinking water (AT group). They were sacrificed after 25 weeks of drinking the treated water. The infection rate with C. albicans in the AT group was significantly higher than in the AL group. The incidence and severity of the squamous cell hyperplasia were enhanced in the AT group compared to the AL group. The proliferative lesions were consistently accompanied by inflammation and C. albicans infection in both groups. SCC was detected in one case in the AT group. These findings demonstrate that tetracycline induces C. albicans infection and enhances forestomach proliferative lesions in alloxan-induced diabetic rats.

Topics: Animals; Anti-Bacterial Agents; Candida albicans; Candidiasis; Carcinoma, Squamous Cell; Cell Proliferation; Diabetes Mellitus, Experimental; Female; Gastric Mucosa; Gastritis; Hyperplasia; Rats; Rats, Inbred Strains; Stomach; Tetracycline

Squamous cell carcinomas and several other cancers have been found to exhibit microarray expression profiles that include genes related to nuclear factor (NF)-kappaB, a signal activated transcription factor that is evolutionarily important in regulating early response gene programs to injury and infection. Inhibition of NF-kappaB by expression of a dominant negative signal phosphorylation site mutant of inhibitor-kappaB, IkappaBalphaM, under a tetracycline inducible promoter, established the role of NF-kappaB as an essential molecular switch modulating multiple genes important in the malignant phenotype. Bioinfomatic analysis of the promoter and coding region of IkappaBalphaM-modulated genes has enabled identification of new candidates with and without known NF-kappaB related motifs for validation and functional studies of their relationship to NF-kappaB. These studies illustrate how microarray data can be used to generate a hypothesis regarding regulation of genes by a specific signal transcription factor, and how genetic mutants and bioinformatic analysis can be used to analyze the relative importance of the regulatory molecule to expression of genes involved in the malignant phenotype.

Topics: Animals; Carcinoma, Squamous Cell; Cell Line, Tumor; Computational Biology; Disease Progression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Head and Neck Neoplasms; Humans; Mice; NF-kappa B; Oligonucleotide Array Sequence Analysis; Signal Transduction; Software; Tetracycline

Mutations in the tumor suppressor gene p53 were found in more than 90% of all human squamous cell carcinomas (SCC). To study the function of p53 in a keratinocyte background, a tetracycline-controlled p53 transgene was introduced into a human SCC cell line (SCC15), lacking endogenous p53. Conditional expression of wild-type p53 protein upon withdrawal of tetracycline was accompanied with increased expression of p21(WAF1/Cip1) resulting in reduced cell proliferation. Flow-cytometric analysis revealed that these cells were transiently arrested in the G1/S phase of the cell cycle. However, when SCC15 cells expressing p53 were exposed to ionizing radiation (IR), a clear shift from a G1/S to a G2/M cell cycle arrest was observed. This effect was greatly depending on the presence of wild-type p53, as it was not observed to the same extent in SCC15 cells lacking p53. Unexpectedly, the p53- and IR-dependent G2/M cell cycle arrest in the keratinocyte background was not depending on increased expression or stabilization of 14-3-3sigma, a p53-regulated effector of G2/M progression in colorectal cancer cells. In keratinocytes, 14-3-3sigma (stratifin) is involved in terminal differentiation and its cell cycle function in this cell type might diverge from the one it fulfills in other cellular backgrounds.

Topics: 14-3-3 Proteins; Biomarkers, Tumor; Carcinoma, Squamous Cell; Cell Cycle; Cell Cycle Proteins; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21; Exonucleases; Exoribonucleases; Gene Expression Regulation, Neoplastic; Humans; Keratinocytes; Neoplasm Proteins; Reverse Transcriptase Polymerase Chain Reaction; Tetracycline; Tumor Cells, Cultured; Tumor Suppressor Protein p53

We report the first case of bullous pemphigoid complicating radiation therapy for vulvar cancer. Shortly after completion of postoperative radiation therapy for a TIN1 vulvar carcinoma, the patient presented with a rash that started within, but continued to extend, well beyond the radiation field. A biopsy of the lesions confirmed the diagnosis of bullous pemphigoid, and she had prompt clinical resolution with systemic tetracycline and steroids.

Topics: Acute Disease; Aged; Anti-Inflammatory Agents; Carcinoma, Squamous Cell; Diagnosis, Differential; Female; Humans; Pemphigoid, Bullous; Radiodermatitis; Steroids; Tetracycline; Vulvar Neoplasms

In chylous fistulas following radical neck dissections, we have found reexploration to be unrewarding, with infrequent identification of a specific leakage site intraoperatively and persistent fluid accumulation postoperatively. As an alternative, we injected tetracycline hydrochloride into the supraclavicular wound bed. This procedure resulted in a rapid, sustained decline in fistula output in two of three cases, avoiding surgical intervention. Tetracycline sclerotherapy has been described for treatment of intrathoracic and other intracavitary fluid collections. We believe that tetracycline sclerotherapy is an effective adjunct in the management of chylous fistulas following radical neck dissections and that this therapy should be attempted before surgical reexploration.

Topics: Adenocarcinoma; Aged; Carcinoma, Squamous Cell; Chyle; Drainage; Female; Fistula; Head and Neck Neoplasms; Humans; Male; Methods; Neck Dissection; Parotid Neoplasms; Postoperative Complications; Sclerosing Solutions; Tetracycline; Time Factors

Actinomycotic infections are unusual, but the actual incidence is likely to be significantly higher than records indicate. The disease may complicate trauma of many types to the respiratory and digestive tracts, including operative procedures. This possibility should encourage more frequent use of anaerobic cultures in inflammatory diseases, particularly posttraumatic, and should prompt consideration of actinomycosis in the differential diagnosis of infections, especially in the cervicofacial area. We report four cases that demonstrate the variable course of this infection. Treatment is highly successful with appropriate use of antibiotics and surgery. A plea is made to use the least expensive, effective antibiotic in view of the prolonged course of therapy that is necessary to eradicate this infection.

Topics: Abscess; Actinomycosis, Cervicofacial; Aged; Ampicillin; Anti-Bacterial Agents; Carcinoma, Squamous Cell; Cephalosporins; Female; Humans; Laryngeal Neoplasms; Male; Middle Aged; Parotid Gland; Penicillin G; Tetracycline; Wounds and Injuries

The case history of a 52-year-old male with weight loss, steatorrhea and arthritis is presented. During clinical pathological conference, Whipple's disease was strongly suggested. The diagnosis could be proved morphologically. Antibiotic therapy with tetracycline 1 g daily for three months caused a prompt improvement. Small bowel biopsy showed disappearance of PAS-containing macrophages.

Topics: Body Weight; Carcinoma, Squamous Cell; Diarrhea; Head and Neck Neoplasms; Humans; Malabsorption Syndromes; Male; Middle Aged; Tetracycline; Whipple Disease

Topics: Acne Vulgaris; Adolescent; Carcinoma, Squamous Cell; Female; Humans; Immunologic Deficiency Syndromes; Palatal Neoplasms; Tetracycline

Topics: Administration, Oral; Adult; Aged; Biopsy; Carcinoma, Adenoid Cystic; Carcinoma, Squamous Cell; Female; Fluorescence; Humans; Injections, Intravenous; Laryngeal Neoplasms; Leukoplakia; Lymphoma; Male; Methods; Middle Aged; Mouth Neoplasms; Neoplasm Metastasis; Pharyngeal Neoplasms; Tetracycline; Time Factors

Topics: Adolescent; Adult; Bacteria; Black People; Carcinoma, Squamous Cell; Female; Granuloma Inguinale; Homosexuality; Humans; Male; Microscopy, Electron; Middle Aged; Penile Neoplasms; Staining and Labeling; Syphilis; Tetracycline

Topics: Biopsy; Carcinoma, Squamous Cell; Cystoscopy; Fluorescence; Follow-Up Studies; Humans; Methods; Tetracycline; Ultraviolet Rays; Urinary Bladder Neoplasms; Urine

Topics: Animals; Carcinoma, Squamous Cell; Fluorescence; Fluorometry; Kidney; Liver; Methods; Microscopy, Fluorescence; Neoplasms, Experimental; Rats; Skin Neoplasms; Spectrophotometry; Tetracycline; Time Factors

Topics: Carcinoma, Basal Cell; Carcinoma, Squamous Cell; Female; Humans; Keratoacanthoma; Methotrexate; Middle Aged; Neoplasm Recurrence, Local; Nose Neoplasms; Tetracycline

Topics: Adenocarcinoma; Carcinoma, Squamous Cell; Cytodiagnosis; Female; Genital Neoplasms, Female; Humans; Microscopy, Fluorescence; Ovarian Neoplasms; Tetracycline; Uterine Cervical Neoplasms; Uterine Neoplasms; Vulvar Neoplasms

Topics: Avitaminosis; Carcinoma, Squamous Cell; Denture, Complete; Gingival Diseases; Hematologic Diseases; Humans; Male; Middle Aged; Mouth Neoplasms; Poisoning; Stomatitis, Aphthous; Tetracycline; Ulcer

Topics: Adult; Carcinoma, Squamous Cell; Diagnosis, Differential; Granuloma Inguinale; Humans; Male; Middle Aged; Penile Diseases; Staining and Labeling; Streptomycin; Tetracycline

Topics: Animals; Benz(a)Anthracenes; Carcinoma, Squamous Cell; Female; Fluorescence; In Vitro Techniques; Injections, Intramuscular; Leukemia, Experimental; Male; Mammary Neoplasms, Experimental; Necrosis; Neoplasms, Experimental; Ovarian Neoplasms; Rabbits; Rats; Tetracycline

Topics: Adolescent; Adult; Aged; Carcinoma, Squamous Cell; Child; Diagnosis, Differential; Fluorescence; Humans; Middle Aged; Skin Diseases; Skin Neoplasms; Tetracycline

Topics: Adenocarcinoma; Carcinoma; Carcinoma, Squamous Cell; Cystoscopy; Fluorescence; Humans; Tetracycline; Ultraviolet Rays; Urinary Bladder Neoplasms