tetracycline and Carcinoma--Small-Cell

Biloma is an extraductular collection of bile within a defined capsular space. Prior reports have documented an association between biloma and abdominal trauma, and between biloma and iatrogenic injury resulting from abdominal surgery, percutaneous catheter drainage, or transhepatic cholangiogram. To our knowledge, bilomas have not previously been associated with lung cancer. We report a case of spontaneous biloma that developed as a complication of small cell lung cancer.

Topics: Aged; Bile; Carcinoma, Small Cell; Hepatomegaly; Humans; Lung Neoplasms; Male; Neoplasm Staging; Organotechnetium Compounds; Peritoneal Cavity; Pyrrolidines; Radionuclide Imaging; Radiopharmaceuticals; Tetracycline; Tomography, X-Ray Computed; Ultrasonography

It is well established that induction of the p53 tumor suppressor protein in cells can lead to either cell cycle arrest or apoptosis. To further understand features of p53 that contribute to these cell responses several p53-null Saos2 and H1299 cell lines were generated that express wild-type or mutant forms of p53, or the cyclin-dependent kinase inhibitor p21/WAF1, under a tetracycline-regulated promoter. Our results show that the cellular level of p53 can dictate the response of the cell such that lower levels of p53 result in arrest whereas higher levels result in apoptosis; nevertheless, DNA damage can heighten the apoptotic response to p53 without altering the protein level of p53 in cells. We also demonstrate that arrest and apoptosis are two genetically separable functions of p53 because a transcriptionally incompetent p53 can induce apoptosis but not arrest, whereas induction of p21/WAF1, which is a major transcriptional target of p53, can induce arrest but not apoptosis. Finally, we show that a full apoptotic response to p53 requires both its amino and carboxyl terminus, and our data suggest that there is synergism between transcription-dependent and -independent functions of p53 in apoptosis. Thus, there are multiple independent cellular responses to p53 that together may account for the extraordinarily high frequency of p53 mutations in diverse types of human tumors. The implications of these results are discussed and a model is proposed.

Topics: Apoptosis; Camptothecin; Carcinoma, Small Cell; Cell Cycle; Cell Division; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; DNA Damage; Enzyme Inhibitors; Humans; Mutation; Osteosarcoma; Tetracycline; Topoisomerase I Inhibitors; Transcription, Genetic; Transcriptional Activation; Tumor Cells, Cultured; Tumor Suppressor Protein p53

Topics: Carcinoma, Bronchogenic; Carcinoma, Small Cell; Cyclophosphamide; Humans; Lung Neoplasms; Neoplasm Metastasis; Tetracycline; Time Factors