tetracycline has been researched along with Carcinoma--Embryonal* in 1 studies
1 other study(ies) available for tetracycline and Carcinoma--Embryonal
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F9 embryonal carcinoma cells engineered for tamoxifen-dependent Cre-mediated site-directed mutagenesis and doxycycline-inducible gene expression.
The study of gene functions in complex genetic environments such as mammalian cells would greatly benefit from systems allowing a tight control of gene expression. The tetracycline-inducible gene expression system and the site-specific Cre/loxP recombination system have gained increasing popularity for conditional expression and gene disruption. To facilitate the analysis of gene functions in a cell autonomous system, we have established an F9 murine embryonal carcinoma cell line, constitutively expressing both the doxycycline-controlled transactivator rtTA and the tamoxifen-dependent Cre recombinase Cre-ER(T). The expression of a reporter gene placed under the control of tetracycline operators was induced about 1000-fold by doxycycline, and tamoxifen-induced excision of a loxP-flanked DNA segment occurred in all cells. This genetically engineered cell line, which allows, upon simple ligand addition, sophisticated genetic manipulations, such as sequential inactivation of loxP-flanked genes, and tightly controlled reexpression of their cDNAs, should be a valuable tool for studying mammalian gene functions. Topics: Carcinoma, Embryonal; Doxycycline; Gene Expression; Genetic Engineering; Humans; Integrases; Mutagenesis, Site-Directed; Receptors, Estrogen; Repressor Proteins; Retinoids; Tamoxifen; Tetracycline; Tumor Cells, Cultured; Viral Proteins | 2000 |