tetracycline and Carcinoma--Ehrlich-Tumor

Electron spin resonance spectra of spin labeled tetracycline (TC-SL) do not show any recognizable partitioning into a lipid bilayer or bulk hydrocarbon solvent, paraffin oil. TC-SL, however, penetrates through the model and biological membranes. It is shown that the rate of permeation depends on membrane composition and increases with temperature. In fluid phase, the rate is greater for saturated dimyristoylphosphatidylcholine than for unsaturated egg yolk phosphatidylcholine membranes. Cholesterol significantly decreases the rate, 30 mol% cholesterol decreases the rate of TC-SL permeation across egg yolk phosphatidylcholine bilayer 8 times at 37 degrees C. The rate of permeation of TC-SL across model membranes is much smaller than the rate for TEMPONE--a compound which slightly partitions into lipid bilayer, and much greater than the rate for TEMPO--choline-a positively charged compound. After addition to the suspension of Ehrlich's ascites tumor cells, the TC-SL is reduced to a non-paramagnetic form. Reduction rate is independent of oxygen concentrations, which led us to suggest that the permeation of TC-SL across the cell plasma membrane is the limiting step of reduction reaction.

Topics: Animals; Carcinoma, Ehrlich Tumor; Electron Spin Resonance Spectroscopy; Kinetics; Liposomes; Mice; Models, Biological; Permeability; Spin Labels; Tetracycline

Topics: Adenine; Animals; Carbon Radioisotopes; Carcinoma, Ehrlich Tumor; Cells; HeLa Cells; Humans; In Vitro Techniques; Oxygen Consumption; Tetracycline; Thymidine; Uridine; Valine

Topics: Acridines; Animals; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; Liver Neoplasms; Mice; Microscopy, Fluorescence; Neoplasms, Experimental; Radiation Effects; Rats; Tetracycline

Topics: Animals; Carcinoma, Ehrlich Tumor; Citrates; Citric Acid Cycle; Culture Techniques; Depression, Chemical; Drug Synergism; Fumarates; Glucose; Ketoglutaric Acids; Lactates; Malates; Oxaloacetates; Oxygen Consumption; Pyruvates; Stimulation, Chemical; Succinates; Tetracycline

Topics: Animals; Carcinoma, Ehrlich Tumor; Cell Line; Chickens; Depression, Chemical; Drug Synergism; Erythrocytes; Glucose; Glycolysis; Lactates; Oxygen Consumption; Stimulation, Chemical; Tetracycline

Topics: Animals; Carcinoma, Ehrlich Tumor; Culture Techniques; Depression, Chemical; Glucose; Glycolysis; Lactates; Mice; Oxygen Consumption; Tetracycline

Topics: Animals; Carcinoma, Ehrlich Tumor; Fluorescence; Methods; Mice; Tetracycline; Ultraviolet Rays

Topics: Aldehydes; Animals; Carcinoma, Ehrlich Tumor; Glycerol; Histological Techniques; In Vitro Techniques; Mice; Microscopy, Fluorescence; Tetracycline

Topics: Animals; Carcinoma, Ehrlich Tumor; Fluorescent Dyes; In Vitro Techniques; Mice; Spectrophotometry; Tetracycline

Topics: Adenoma; Aminosalicylic Acid; Aminosalicylic Acids; Animals; Anti-Bacterial Agents; Antineoplastic Agents; Antitubercular Agents; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; Chloramphenicol; Isonicotinic Acids; Leukemia; Leukemia, Experimental; Liver Neoplasms; Neoplasms; Neoplasms, Experimental; Oleandomycin; Pharmacology; Pyrazinamide; Research; Rolitetracycline; Sarcoma; Sarcoma, Experimental; Sarcoma, Yoshida; Streptomycin; Tetracycline

Topics: Acridines; Animals; Antineoplastic Agents; Carcinoma; Carcinoma, Ehrlich Tumor; Chemical Phenomena; Chemistry; Coloring Agents; Fluoresceins; Fluorescence; Merbromin; Mercury; Mice; Porphyrins; Protein Synthesis Inhibitors; Research; Tetracycline

Topics: Animals; Anti-Bacterial Agents; Carcinoma; Carcinoma, Ehrlich Tumor; Fluorescence; Fluorescent Dyes; Humans; Mice; Microscopy; Microscopy, Fluorescence; Neoplasms; Neoplasms, Experimental; Oxytetracycline; Rats; Research; Tetracycline