tetracycline and Bone-Neoplasms

We report the case of a 15-year-old male patient presenting with persistent wrist pain after surgical treatment of a triquetral osteoid osteoma. The patient was found to have a persistent nidus. These tumors are difficult to observe intraoperatively or through fluoroscopy, limiting adequate resection and resulting in continued pain.. The nidus was excised successfully through an unusual technique using tetracycline for intraoperative identification. The patient remains asymptomatic without recurrence at 31 months postoperative. Difficulties visualizing and removing carpal lesions leading to recurrence are described.

Topics: Adolescent; Bone Neoplasms; Humans; Male; Orthopedic Procedures; Osteoma, Osteoid; Reoperation; Tetracycline; Tomography, X-Ray Computed; Triquetrum Bone

We report a 62-year-old man who presented with pain on the right side of his hip. CT revealed destructive masses in the right femur and left ilium. Histological examination indicated metastases from hepatocellular carcinoma, and further investigations revealed the primary tumor in the liver. Hepatobiliary scintigraphy using 99mTc N-pyrydoxyl-5-methyltryptophan and fused SPECT/CT clearly showed abnormal accumulation in these bone metastases from hepatocellular carcinoma.

Topics: Biliary Tract; Bone Neoplasms; Carcinoma, Hepatocellular; Humans; Liver; Liver Neoplasms; Male; Middle Aged; Multimodal Imaging; Organotechnetium Compounds; Positron-Emission Tomography; Pyrrolidines; Tetracycline; Tomography, X-Ray Computed

Bone tumor is a notoriously difficult disease to manage, requiring frequent and heavy doses of systemically administered chemotherapy. Targeting anticancer drug to the bone after systemic administration may provide both greater efficacy of treatment and less frequent administration. In this paper, a series of bone targeting Asp oligopeptides 5-fluorouracil conjugates have been synthesized in a convergent approach and well characterized by NMR and MS techniques. Their hydroxyapatite (HAP) affinity, drug release and cytotoxicity characteristics were evaluated in in vitro conditions. All the prodrugs were water soluble and exhibited high affinity to HAP .The efficient release of the active drug moiety occurring by the cleavage of different linkage in physiological conditions significantly reduced the number of viable human cancer cells. From in vivo distribution, we get these compounds with high bone-selectivity and long halflife. These results provided an effective entry to the development of new bone targeting chemotherapeutic drugs.

Topics: Animals; Antineoplastic Agents; Bone Neoplasms; Cell Line, Tumor; Cell Survival; Chromatography, High Pressure Liquid; Drug Delivery Systems; Fluorouracil; Humans; Mice; Molecular Structure; Prodrugs

Osteoid osteoma is infrequently encountered in the carpal bones. Its typical radiological features are not usually observed in this localization, which may result in delayed diagnosis. Since incomplete removal of the nidus may result in persistance or recurrence of the symptoms, other methods of locating the tumour during the resection should be used. We report a case of an osteoid osteoma of the triquetrum which, after incomplete initial resection and several revisions, developed instability of the carpus which required a limited intracarpal arthrodesis. A screw fragment left after implant extraction limited the imaging possibilities to study the persistent nidus. Intra-operative localization of the nidus with tetracycline dye and its visualization with ultraviolet light facilitated the complete removal.

Topics: Adult; Bone Neoplasms; Carpal Bones; Female; Fluorescence; Fluoroscopy; Humans; Intraoperative Period; Osteoma, Osteoid; Reoperation; Tetracycline

Topics: Bone Neoplasms; Carcinoma, Hepatocellular; Clavicle; Humans; Ilium; Liver Neoplasms; Male; Middle Aged; Organotechnetium Compounds; Pyrrolidines; Radionuclide Imaging; Radiopharmaceuticals; Ribs; Scapula; Skull; Technetium Tc 99m Medronate; Tetracycline

To study the effects of XW630 on bone formation in overiectomized (OVX) rats and in human osteoblast-like cell line TE85.. Bone histomorphometric analysis was performed with undecalcified bone sections and tetracycline intraperitoneally labeling.. Compared with that of OVX rats, the static data of trabecular bone volume (TBV)/total tissue volume (TTV), TBV/sponge bone volume (SBV) and mean trabecular plate density (MTPD) were enhanced while mean trabecular plate spacing (MTPS) decreased after treated with XW630 for 13w. The dynamic data of single-labeled surface [Sfract(s)], double-labeled surface [Sfract(d)], Sfract(d+1/2s), trabecular osteoid surface (TOS) and bone formation rate in tissue level (Svf) were increased and osteoid maturation period (OMP) shortened in XW630 group. In osteoblast-like cells, both 3H-thymidine incorporation and cell count increased after treated with XW630 for 48. Treated with XW630 for 12 approximately 18h, inducible nitric oxide synthase (iNOS) activity and cGMP content increased in time-dependent manners.. XW630 enhanced bone activation frequency and increased trabecular connectivity, stability, and strength. The cellular mechanism related to effects of XW630 on bone formation in ovariectomized rats.

Topics: Animals; Bone Neoplasms; Cell Division; Cyclic GMP; Estrone; Female; Femur; Humans; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Osteoblasts; Osteogenesis; Osteosarcoma; Ovariectomy; Piperazines; Rats; Rats, Wistar; Tetracycline; Tetracyclines; Tumor Cells, Cultured

To study the effects of 2-[3-estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) in human osteoblast-like cell line TE85.. [3H]Thymidine incorporation and cell count for cell proliferation, radioimmunoassay for cyclic GMP (cGMP) content, and monitoring the conversion of [3H]arginine for inducible nitric-oxide synthase (iNOS) activity assay.. After treatment with XW630 for 48 h, [3H]thymidine incorporation and cell numbers increased by 62.7% and 96.9%, respectively. NG-monomethyl-L-arginine (L-NMMA, an NOS inhibitor) induced a concentration-dependent inhibitory effect on the proliferation after treatment for 48 h. The inhibitory effect was prevented partially by XW630 (1.0 nmol.L-1). After treatment with XW630 for 12-48 h, iNOS activity and cGMP concentration increased in time-dependent manners.. XW630 stimulated cell proliferation, enhanced iNOS activity and cGMP content in human osteoblast-like cell line TE85.

Topics: Bone Neoplasms; Cell Division; Cyclic GMP; Estrogens, Conjugated (USP); Estrone; Humans; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Osteoblasts; Osteosarcoma; Piperazines; Tetracycline; Tetracyclines; Tumor Cells, Cultured

Bone metastases are a common complication in prostate and breast cancer patients. It leads to extensive morbidity and eventually mortality. Matrix metalloproteinases are implicated in various steps of development of metastasis, through their ability to degrade the extracellular matrix. Increased matrix metalloproteinase activity of tumor cells has been associated with a higher metastatic potential. Inhibitors of metalloproteinases have been shown to effectively reduce or prevent the formation of metastases. The family of tetracyclines is able to inhibit matrix metalloproteinase activity through chelation of the zinc ion at the active site of the enzyme. Using tumor cell lines relevant to bone metastases, i.e. PC-3, MDA-MB-231, Hs696, B16/F1, we showed that tetracycline and derivatives of tetracycline, namely doxycycline and minocycline, also induced cytotoxicity. The effective concentrations are relatively high for plasma, but are clinically achievable in the bone, since tetracyclines are osteotropic. All four bone-metastasizing tumor cells produced and secreted various matrix metalloproteinases. Doxycycline was able to inhibit the activity of 72- and 92-kDa type IV collagenase secreted by bone-metastasizing cells by 79-87%. These characteristics could make tetracycline a unique candidate as a therapeutic agent to prevent bone metastases in cancer patients with a high likelihood for development of bone metastasis. Studies using animal models of experimental bone metastasis will be necessary to confirm this.

Topics: Adenocarcinoma; Animals; Anti-Bacterial Agents; Blotting, Western; Bone Neoplasms; Breast Neoplasms; Cell Survival; Collagenases; Culture Media, Conditioned; Doxycycline; Extracellular Matrix; Gelatinases; Humans; Male; Matrix Metalloproteinase 1; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Matrix Metalloproteinase Inhibitors; Melanoma; Metalloendopeptidases; Mice; Minocycline; Prostatic Neoplasms; Protease Inhibitors; Tetracycline; Tetracyclines; Tumor Cells, Cultured

The severity of pain and functional disability contrast with the benignity and small size which characterize the osteoid osteoma. This study evaluates the clinical and histological efficiency of an intraoperative localization of the nidus by the tetracycline fluorescence test which is based on histomorphometric bone labeling techniques.. 17 out 25 osteoïd osteoma, operated between 1987 and 1995, had tetracycline fluorescence test. The patients received 1 gram orally during each of the 3 pre-operative days. Fluorescence under ultraviolet light was assessed on the removed specimen, and on the tumor site, before and after surgical resection.. At a mean follow-up of 39 months (12-85), 15 of the 17 patients (88 per cent) were painless. The result was always acquired within the first weeks after procedure. 2 patients had a primary failure due to incomplete removal of fluorescent nidus, and one was reoperated with success after one year. One patient had a false negative test although there was histological evidence of osteoid osteoma on the removed tissues with a complete relief of pain. In summary, the test was helpful in 14 cases (82 per cent) guiding "en bloc" resection or curetage, and allowing 70 per cent of histological nidus diagnosis.. This test is easy to carry out by pre-operative ingestion of tetracycline and only requires an ultra-violet light source. It demonstrated a good reliability which can be compared with that of more complex radio-labeling techniques or CT guided drill resections which impede histological confirmation in half of the cases. The fluorescence test allows a secure and precise procedure without complication, and we use it mainly for cancellous and superficial osteoid osteoma, and for revision of previous failed excisions. When the nidus is cortical and deeply located, radioguided techniques must be discussed.

Topics: Adolescent; Adult; Bone Neoplasms; Female; Fluorescence; Humans; Intraoperative Period; Male; Middle Aged; Osteoma, Osteoid; Radiography; Tetracycline

The diagnostic value of whole body scanning using 99mTc-N-pyridoxylmethyltryptophan (PMT) was evaluated in 16 patients with bone metastases from hepatocellular carcinoma, in comparison with 99mTc-MDP. Of the 72 known lesions of bone metastases, 63 (87.5%) were detected by 99mTc-PMT scintigraphy, which demonstrated increased uptake of radionuclide. However, 99mTc-MDP bone scintigraphy detected only 45 lesions (62.5%), which were shown as increased, decreased, or mixed patterns of uptake. Thus 99mTc-PMT scintigraphy was more sensitive than 99mTc-MDP bone scintigraphy. In addition, the latter showed poor specificity because of its high false positive rate due to degenerative change. All lesions undetected by 99mTc-PMT scintigraphy were located in areas that overlapped the liver or bowel activity. In conclusion, it is recommended that whole body 99mTc-PMT scintigraphy be combined with 99mTc-MDP bone scintigraphy for the detection of bone metastases from hepatocellular carcinoma.

Topics: Aged; Aged, 80 and over; Bone Neoplasms; Carcinoma, Hepatocellular; Female; Humans; Japan; Liver Neoplasms; Male; Middle Aged; Organotechnetium Compounds; Pyrrolidines; Radionuclide Imaging; Retrospective Studies; Tetracycline; Tetracyclines

The purposes of this study were (1) to evaluate the various methods of preoperative staging of osteoid osteomas, (2) to compare the different methods of intraoperative localization and excision of the tumor, and (3) to develop a clinical strategy for the treatment of persistent (recurrent) lesions. From 1978 until 1986, 14 consecutive patients had excision of osteoid osteomas. All patients' operative reports, roentgenograms, bone scans, computed tomographic scans and histologic studies were reviewed. With an average follow-up period of 94.5 months, 13 of 14 patients were cured with one operative procedure. One patient required two procedures. No patient developed a recurrence. Eleven of 14 niduses were confirmed on hematoxylin and eosin sections. Computed tomographic scan was the most useful preoperative study in localizing the nidus. It helped determine the surgical approach and the portion of bone to be resected. Intraoperative nuclear scanning (IONS) is a reliable technique in confirming complete removal of the nidus, with no increase in operative time. Intraoperative scanning did not appreciably affect the amount of bone resected. Recurrent tumors can be reliably resected, with a high cure rate, by careful preoperative staging and with use of IONS.

Topics: Adolescent; Adult; Bone Neoplasms; Child; Child, Preschool; Female; Fluorescence; Gamma Cameras; Humans; Male; Monitoring, Intraoperative; Neoplasm Recurrence, Local; Neoplasm Staging; Osteoma, Osteoid; Radionuclide Imaging; Tetracycline; Tomography; Tomography, X-Ray Computed

Nine patients with a clinical and radiographic diagnosis of osteoid-osteoma received 750 to 4,000 milligrams of tetracycline preoperatively. Immediate examination of the surgically removed specimens under ultraviolet light demonstrated fluorescence of the nidus in all nine patients. Reactive and normal bone did not fluoresce. This simple technique permits quick, easy, economical, and sure verification that the nidus has been excised.

Topics: Adolescent; Adult; Bone Neoplasms; Child; Female; Fluorescence; Humans; Intraoperative Care; Male; Osteoma, Osteoid; Preoperative Care; Tetracycline; Ultraviolet Rays

Twelve patients with tumors and three with osteitis of the extremities were studied using 99mTc-labelled tetracycline in order to image the process with gamma camera. The diagnosis of all cases were verified histologically. The labelled tetracycline was found to give positive images for the most malignant tumors and active osteitis which together comprised about half of the studied cases.

Topics: Bone Neoplasms; Extremities; Humans; Osteitis; Osteosarcoma; Radionuclide Imaging; Soft Tissue Neoplasms; Technetium; Tetracycline; Tibia

A prospective study of 40 consecutive classic primary osteosarcomas employing tomography, angiography, radio-scanning, tetracycline labelling, macroscopic and microscopic study revealed that in 10 (25%) "skip" metastases were found. In 8 the "skips" were completely unsuspected prior to computation. It is evident that patients with "skips" are more prone to local recurrence and have a worse prognosis following amputation than those without such satellite lesions. Through-bone amputation entails a considered risk of leaving micro-foci of tumor; proximal disarticulation does not obviate the risk of cross joint "skips" when the lesion is in a sub-articular site. What affect such residual foci has upon the effectiveness of adjunct therapy and conversely whether such therapy will permit more conservative surgery by suppressing residual "skips" is an important but unanswered question.

Topics: Adolescent; Adult; Aged; Amputation, Surgical; Bone Neoplasms; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Neoplasm Metastasis; Osteosarcoma; Prospective Studies; Tetracycline

Topics: Adult; Aged; Ascitic Fluid; Bone Neoplasms; Breast Neoplasms; Cardiovascular Diseases; False Negative Reactions; False Positive Reactions; Female; Filtration; Gastrointestinal Neoplasms; Humans; Male; Membranes, Artificial; Methods; Middle Aged; Neoplasms; Ovarian Neoplasms; Pleural Effusion; Prostatic Neoplasms; Respiratory Tract Diseases; Respiratory Tract Neoplasms; Testicular Neoplasms; Tetracycline; Tuberculosis

Topics: Adolescent; Adult; Animals; Antigens, Neoplasm; Blood Proteins; Bone Neoplasms; Child; Child, Preschool; Epitopes; Female; Femoral Neoplasms; Fluoresceins; Humans; Immune Sera; Immunity, Active; Immunity, Maternally-Acquired; Immunization, Passive; Immunodiffusion; Immunoelectrophoresis; Immunotherapy; Infant; Leukocyte Count; Lung Neoplasms; Lymphocyte Activation; Lymphocytes; Male; Neoplasm Metastasis; Neoplasm Transplantation; Osteosarcoma; Rabbits; Skin Tests; Tetracycline; Tibia

Topics: Adult; Bone Neoplasms; Cervical Vertebrae; Cobalt Isotopes; Cyclophosphamide; Humans; Lung Neoplasms; Male; Metacarpus; Neoplasm Metastasis; Nose Neoplasms; Petrous Bone; Radiography; Sarcoma, Ewing; Skull Neoplasms; Tetracycline; Thoracic Vertebrae

Topics: Adult; Animals; Bone and Bones; Bone Neoplasms; Chondrosarcoma; Fluorescence; Humans; Infection Control; L Forms; Male; Mice; Pancreatitis; Pericarditis; Pneumonia; Postoperative Complications; Rabbits; Tetracycline

Topics: Blood Circulation; Bone Neoplasms; Carotid Arteries; Coloring Agents; Fluorescence; Fluorescent Dyes; Humans; Injections, Intra-Arterial; Lip Neoplasms; Neoplasms; Tetracycline

Topics: Bone Neoplasms; Fluorescence; Humans; Neoplasms; Osteosarcoma; Sarcoma; Tetracycline

Topics: Bone Neoplasms; Colon; Fluorescence; Intestinal Neoplasms; Neoplasms; Protein Synthesis Inhibitors; Stomach Neoplasms; Tetracycline

Topics: Bone and Bones; Bone Neoplasms; Fibrosarcoma; Humans; Neoplasms; Osteosarcoma; Tetracycline