tetracycline and Bone-Diseases

In young growing rats, fed the lathyrogenic agent aminoacetonitrile for 3 weeks, characteristic skeletal changes developed resembling the chronic state of osteolathyrism. In both lathyritic and untreated control rats, the femoral head, the distal end of the femur, the proximal tibial end, the thalocalcanean joint and the first lumbar vertebra were investigated using undecalcified thin sections and ground sections. The following methods were employed: quantitative morphometrical analysis of bone structure and bone remodelling of the lumbar vertebra, histochemical demonstration of the composition of polysaccharides, detection of the course of collagenous fibres in cartilage and bone by the polarizing microscope, tetracycline-labelling and fluorescence microscopical determination of epiphyseal longitudinal growth and bone formation activity, photometrical quantification of microradiographies and computer-aided determination of the mineral content of bone.

Topics: Aminoacetonitrile; Animals; Bone Diseases; Bone Resorption; Calcinosis; Disease Models, Animal; Exostoses, Multiple Hereditary; Femur Head; Male; Osteoblasts; Osteoclasts; Rats; Spine; Tetracycline; Tibia

Topics: Adrenal Cortex Hormones; Antacids; Antibiotics, Antineoplastic; Anticoagulants; Anticonvulsants; Bone and Bones; Bone Diseases; Cadmium; Cathartics; Chronic Disease; Dactinomycin; Diuretics; Drug-Related Side Effects and Adverse Reactions; Fluorides; Humans; Hyperparathyroidism, Secondary; Iron; Lead; Methotrexate; Plicamycin; Radium; Tetracycline; Vitamin A; Vitamin D

Topics: Absorptiometry, Photon; Adult; Aged; Aging; Bone Diseases; Bone Resorption; Calcium; Child; Estrogens; Female; Humans; Hydroxyproline; Hyperparathyroidism; Joint Diseases; Male; Microradiography; Middle Aged; Osteitis Deformans; Osteomalacia; Osteoporosis; Parathyroid Glands; Physical Therapy Modalities; Spine; Tetracycline

Topics: Animals; Bone and Bones; Bone Diseases; Bone Regeneration; Bone Resorption; Calcification, Physiologic; Histocytochemistry; Humans; Kinetics; Tetracycline

Topics: Bone Development; Bone Diseases; Child, Preschool; Dentition; Enzyme Repression; Female; Humans; Infant; Infant, Newborn; Maternal-Fetal Exchange; Nails; Pregnancy; Pregnancy Complications, Infectious; Protein Biosynthesis; Skin Diseases; Tetracycline; Tooth Discoloration

Tetracyclines (TCs) are a class of broad-spectrum antibiotics with diverse pharmacotherapeutic properties due to their various functional groups being attached to a common core structure. Beyond their antibacterial activity, TCs trigger pleiotropic effects on eukaryotic cells, including anti-inflammatory and potentially osteogenic capabilities. Consequently, TCs hold promise for repurposing in various clinical applications, including bone-related conditions. This study presents the first comprehensive comparison of the in vitro osteogenic potential of four TCs-tetracycline, doxycycline, minocycline, and sarecycline, within human mesenchymal stem cells. Cultures were characterized for metabolic activity, cell morphology and cytoskeleton organization, osteogenic gene expression, alkaline phosphatase (ALP) activity, and the activation of relevant signaling pathways. TCs stimulated actin remodeling processes, inducing morphological shifts consistent with osteogenic differentiation. Osteogenic gene expression and ALP activity supported the osteoinduction by TCs, demonstrating significant increases in ALP levels and the upregulation of RUNX2, SP7, and SPARC genes. Minocycline and sarecycline exhibited the most potent osteogenic induction, comparable to conventional osteogenic inducers. Signaling pathway analysis revealed that tetracycline and doxycycline activate the Wnt pathway, while minocycline and sarecycline upregulated Hedgehog signaling. Overall, the present findings suggest that TCs promote osteogenic differentiation through distinct pathways, making them promising candidates for targeted therapy in specific bone-related disorders.

Topics: Anti-Bacterial Agents; Bone Diseases; Doxycycline; Hedgehog Proteins; Heterocyclic Compounds; Humans; Immunologic Factors; Mesenchymal Stem Cells; Minocycline; Osteogenesis; Tetracycline

We present an image of a patient's skull characterized by dark, irregular discoloration. This was discovered incidentally in a 66-year-old man who underwent craniotomy for resection of a glioblastoma. This image demonstrates cranial black bone disease. This is an abnormal bone pigmentation associated with long-term tetracycline use, as occurred in this patient.

Topics: Aged; Anti-Bacterial Agents; Bone Diseases; Craniotomy; Glioblastoma; Humans; Male; Skull; Tetracycline; Time; Tomography, X-Ray Computed

To review the phenomenon and implications of temporal bone and craniofacial bone staining in the context of prolonged exposure to tetracycline antibiotic.. Case report and literature review.. A 52-year-old male with a 5-year history of tetracycline use presented to undergo tympanomastoidectomy and was found to have an unusual rusty green pigmentation of the entire aspect of the exposed temporal bone. A literature review revealed more than 20 cases of tetracycline-induced pigmentation of intraoral maxillary and mandibular bone, and 2 prior cases involving the cranial bones.. Tissue and organ pigmentation is an unexpected and unfavourable consequence of the use of tetracyclines, particularly minocycline. Tetracycline is contraindicated in children because of the risk for dysosteogenesis and enamel hypoplasia. In adults, although the unusual staining may present as an unexpected dilemma upon surgical exposure, current research shows no significant clinical consequences for this type of pigmentation.

Topics: Anti-Bacterial Agents; Bone Diseases; Humans; Male; Middle Aged; Pigmentation; Pigmentation Disorders; Temporal Bone; Tetracycline

A radial segmental defect model of a rabbit was used to study the restoration effect on defects treated with chitosan-coated pressed calcium sulfate pellets combined with recombinant human bone morphogenetic protein-2 (rhBMP-2), coated pressed calcium sulfate pellets, and uncoated pressed calcium sulfate pellets. Nothing was implanted in the control group. After 4, 8, and 12 weeks, the results indicated that coated pressed calcium sulfate pellets combined with rhBMP-2 and coated pressed calcium sulfate pellets facilitated new bone formation on defected bones and that, particularly, the coated pressed calcium sulfate pellets combined with rhBMP-2 was more effective than the coated pressed calcium sulfate pellet. Histologic and tetracycline fluorimetric findings showed that the osteogenesis mechanism of chitosan-coated pressed calcium sulfate pellets is membrane bone formation, and the pellets showed slightly slower resorption that closely coincides with the growth rate of new bone.

Topics: Absorbable Implants; Animals; Bone Density; Bone Diseases; Bone Marrow; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Bone Substitutes; Calcification, Physiologic; Calcium Sulfate; Chitosan; Coated Materials, Biocompatible; Connective Tissue; Diaphyses; Disease Models, Animal; Fluorescent Dyes; Fluorometry; Humans; Osteogenesis; Rabbits; Radius; Recombinant Proteins; Stress, Mechanical; Tetracycline; Time Factors; Transforming Growth Factor beta

The purpose of this study was to investigate the incorporation of fresh frozen irradiated membranous allogeneic bone grafts into critical size calvarial defects in the rabbit. Fifteen rabbits had calvarial defects prepared. Twelve rabbits received allogeneic grafts and three received autogenous bone grafts. The rabbits were sacrificed at 9 and 12 months postoperatively, and the specimens were examined radiologically, histopathologically and with fluorescence microscopy. Neovascularization, bone marrow regeneration and new bone formation was evident throughout the grafts however revitalization of the entire graft was incomplete at 12 months. This study revealed that the FFI membranous grafts were well incorporated into rabbit calvarial defects.

Topics: Animals; Anthraquinones; Bone Diseases; Bone Marrow; Bone Matrix; Bone Regeneration; Bone Remodeling; Bone Transplantation; Cryopreservation; Fluoresceins; Fluorescent Dyes; Microscopy, Fluorescence; Neovascularization, Physiologic; Osteoblasts; Osteoclasts; Osteogenesis; Rabbits; Radiography; Skull; Statistics as Topic; Statistics, Nonparametric; Tetracycline; Time Factors; Transplantation, Autologous; Transplantation, Homologous

This is an experimental study on the potential of revascularized periosteal allografts for repair of bony defects. Beijing long-eared white rabbits were used as donors and immunologically incompatible Chinchilla rabbits were used as recipients. The periosteum was stripped off from the femurs of the 6 donor rabbits and transplanted to bony defects of 1.2 cm over the radius of the 12 recipients using microsurgical anastomoses of the blood vessels. In the control group of 8 rabbits the same radial bony defects were produced without the transplantation of periosteal grafts. Immunosuppressants were administered after surgery. The animals were observed for 4 months using a series of radiography, histomorphology, fluorochrome labelling and electron microscopy. Results revealed no bony healing of the defects in the control group, but 9 of the 12 defects healed in the experimental group. The results indicated revascularized periosteal allografts were able to form new bone and heal the 1.2 cm radial bone defect in rabbits.

Topics: Anastomosis, Surgical; Animals; Bone Diseases; Bone Marrow; Bone Transplantation; Cartilage; Female; Haversian System; Male; Microscopy, Electron; Microscopy, Fluorescence; Osteoblasts; Osteocytes; Osteogenesis; Periosteum; Rabbits; Radius; Tetracycline; Transplantation, Homologous; Vascular Patency; Wound Healing

Quantitative histomorphometric analyses of iliac crest biopsy specimens were performed after tetracycline double-labeling in 41 normal individuals, 20 hyper- and 10 hypothyroid patients, 18 patients with primary hyperparathyroidism, 20 epileptic patients receiving long-term anticonvulsant therapy, and 17 patients after jejunoileal bypass for morbid obesity. The mineralization lag time in trabecular bone or the period of time between apposition and subsequent mineralization of osteoid was calculated from the bone formation rate at BMU level (Basic Multicellular Unit) and the mean width of osteoid seams. The mineralization lag time was 8-52 days (median 21 days) in normal individuals and showed no variation with sex or age. The mineralization lag time was shortened in hyperthyroidism, normal in anticonvulsant bone disease and in primary hyperparathyroidism, and markedly prolonged in hypothyroidism and following jejunoileal bypass. Among all individuals an inverse hyperbolic relation (r = 0.94, p less than 0.001) was found between the mineralization lag time and the average cellular activity of the osteoblasts.

Topics: Adult; Aged; Biopsy; Bone and Bones; Bone Diseases; Female; Humans; Hyperparathyroidism; Hyperthyroidism; Hypothyroidism; Ileum; Ilium; Male; Middle Aged; Minerals; Obesity; Osteoblasts; Osteomalacia; Phenytoin; Postoperative Complications; Tetracycline; Time Factors

Fluoride-18 scintigraphy is a simple, safe method for evaluating perfusion to bone, and obviates objections to earlier techniques. The scintillation camera with positron detection represents a unique instrumentation system because of its tomographic effect, relative insensitivity to vascular radioactivity in the surrounding skeleton, and excellent spatial resolution despite low counting efficiency. In 75 patients with avascular disorders about the hip, knee, elbow, foot and hand, the impressions obtained from fluoride-18 scintigraphy were correlated with clinical findings and roentgenograms and when possible confirmed by tetracycline labeling and histological examination. Fluoride-18 scintigraphy was useful: in determining the vascularity of the femoral head following femoral neck fractures; in determining results of treatment; and in diagnosing early the various avascular necrotic disorders of the bone.

Topics: Adolescent; Aged; Bone and Bones; Bone Diseases; Child; Female; Femoral Neck Fractures; Fluorescence; Fluorine; Humans; Male; Necrosis; Osteochondritis; Radioisotopes; Radionuclide Imaging; Tetracycline; Wrist Injuries

Topics: Acute Disease; Anti-Bacterial Agents; Bone Diseases; Clindamycin; Drug Therapy, Combination; Humans; Lincomycin; Moscow; Orthopedics; Penicillin G; Penicillin Resistance; Penicillins; Postoperative Complications; Pseudomonas Infections; Staphylococcal Infections; Streptomycin; Tetracycline; Wound Infection; Wounds and Injuries

Topics: Bone and Bones; Bone Diseases; Humans; Methods; Microscopy, Fluorescence; Tetracycline

Topics: Air Sacs; Alkaline Phosphatase; Animals; Bone Diseases; Calcium; Femur; Locomotion; Microscopy, Electron, Scanning; Periosteum; Phosphorus; Poultry Diseases; Selenium; Tetracycline; Tibia; Turkeys; Vitamin E

Topics: Adolescent; Adult; Aged; Bone and Bones; Bone Diseases; Drug Combinations; Female; Fingers; Hand; Humans; Injections; Male; Middle Aged; Morpholines; Oleandomycin; Osteomyelitis; Paronychia; Suppuration; Tetracycline

Topics: Acidosis; Acute Kidney Injury; Animals; Bone Diseases; Bone Regeneration; Bone Resorption; Chronic Kidney Disease-Mineral and Bone Disorder; Hyperplasia; Male; Nephrectomy; Osteoclasts; Osteomalacia; Parathyroid Glands; Rats; Tetracycline; Tibia; Time Factors; Uremia

Topics: Adult; Aged; Bone Diseases; Calcium; Calcium Isotopes; Calcium Metabolism Disorders; Child, Preschool; Demeclocycline; Diet Therapy; Dietary Proteins; Female; Glutens; Humans; Hydrochlorothiazide; Infant; Kinetics; Male; Methandrostenolone; Middle Aged; Osteoporosis; Rickets; Tetracycline; Vitamin D

Topics: Biopsy; Bone Development; Bone Diseases; Bone Resorption; Cartilage; Child, Preschool; Fractures, Ununited; Humans; Microradiography; Osteogenesis; Pseudarthrosis; Tetracycline; Time Factors

Topics: Antibodies; Bacteria; Bone Diseases; Child, Preschool; Female; Gentamicins; Granuloma; Humans; Lymphatic Diseases; Male; Necrosis; Tetracycline; Tonsillitis

Topics: Abscess; Actinomyces; Actinomycosis; Bone Diseases; Drug Resistance, Microbial; Erythromycin; Female; Humans; Middle Aged; Radiography; Skin Diseases, Infectious; Tetracycline

Topics: Biopsy; Birefringence; Bone and Bones; Bone Diseases; Collagen; Hand; Haversian System; Humans; Knee; Male; Microscopy, Fluorescence; Microscopy, Polarization; Middle Aged; Osteoblasts; Osteoclasts; Osteomalacia; Radiography; Ribs; Tetracycline

Topics: Acute Disease; Animals; Bone Development; Bone Diseases; Bone Resorption; Kidney Failure, Chronic; Male; Nephrectomy; Osteogenesis; Rats; Tetracycline; Tibia; Time Factors; Uremia

Topics: Animals; Bone Diseases; Bone Resorption; Calcitonin; Calcium; Female; Fibula; Hindlimb; Immobilization; Microradiography; Organ Size; Ossification, Heterotopic; Rabbits; Swine; Tendons; Tetracycline; Tibia

Topics: Adult; Age Factors; Anemia, Sickle Cell; Bone Diseases; Female; Femoral Fractures; Femur Head; Fluorescence; Follow-Up Studies; Humans; Male; Microscopy, Polarization; Middle Aged; Necrosis; Osteogenesis; Radiography; Sex Factors; Synovial Fluid; Synovitis; Tetracycline; Uric Acid; X-Ray Diffraction

Topics: Adolescent; Adult; Aged; Animals; Bone and Bones; Bone Development; Bone Diseases; Child; Child, Preschool; Dogs; Female; Haversian System; Humans; Male; Middle Aged; Tetracycline

Topics: Animals; Bone Diseases; Diet; Femur; Fluoresceins; Male; Osteoblasts; Osteogenesis; Radius; Swine; Tetracycline; Vitamin A

Topics: Adult; Bone and Bones; Bone Development; Bone Diseases; Female; Humans; Kidney Function Tests; Male; Middle Aged; Renal Dialysis; Tetracycline

Topics: Aged; Animals; Bone and Bones; Bone Diseases; Bone Resorption; Cortisone; Female; Fluorides; Fractures, Bone; Heparin; Humans; Male; Osteitis Deformans; Osteoporosis; Otosclerosis; Phosphates; Rats; Sodium; Tetracycline

Topics: Alkaline Phosphatase; Bone Diseases; Calcium; Humans; Hydroxyproline; Microscopy, Electron; Phosphorus; Radioisotopes; Tetracycline

Topics: Alkaline Phosphatase; Bone Development; Bone Diseases; Child; Female; Humans; Ribs; Tetracycline

Topics: Bone and Bones; Bone Diseases; Bone Resorption; Fluorescence; Humans; In Vitro Techniques; Minerals; Osteoblasts; Osteoclasts; Osteocytes; Phagocytosis; Tetracycline

Topics: Bone Diseases; Humans; Microradiography; Microscopy, Fluorescence; Tetracycline

Topics: Adolescent; Adult; Age Factors; Aged; Bone and Bones; Bone Development; Bone Diseases; Child; Child, Preschool; Densitometry; Female; Humans; Ilium; Infant; Lumbar Vertebrae; Male; Microscopy, Fluorescence; Middle Aged; Ribs; Sex Factors; Tetracycline

Topics: Anti-Bacterial Agents; Bone Diseases; Child; Humans; Tetracycline; Tetracyclines; Tooth; Tooth Diseases; Toxicology

Topics: Anti-Bacterial Agents; Bone Diseases; Eosinophilic Granuloma; Humans; Protein Synthesis Inhibitors; Tetracycline

Topics: Ankle; Biopsy; Blood Chemical Analysis; Bone Diseases; Fluorescence; Genetics, Medical; Hand Deformities; Hypertension; Hypertension, Renal; Joint Diseases; Kidney Diseases; Kidney Function Tests; Osteolysis; Pathology; Radiography; Surgical Procedures, Operative; Tetracycline; Urine; Wrist

Topics: Bone and Bones; Bone Diseases; Humans; Microradiography; Minerals; Osteoblasts; Osteocytes; Pathology; Tetracycline

Topics: Adolescent; Aging; Biopsy; Bone and Bones; Bone Diseases; Bone Diseases, Metabolic; Haversian System; Histology; Humans; Osteogenesis; Ribs; Tetracycline

Topics: Anti-Bacterial Agents; Bone Diseases; Protein Synthesis Inhibitors; Radiation; Radiation Injuries; Tetracycline