tetracarboxyphenylporphine and Neoplasms

The therapeutic effect of traditional chemodynamic therapy (CDT) agents is severely restricted by their weakly acidic pH and glutathione (GSH) overexpression in the tumor microenvironment. Here, fusiform-like copper(II)-based tetrakis(4-carboxy phenyl)porphyrin (TCPP) nanoscale metal-organic frameworks (nMOFs) were designed and constructed for the first time (named PCN-224(Cu)-GOD@MnO

Topics: Animals; Antineoplastic Agents; Cell Line, Tumor; Cell Survival; Copper; Female; Glucose Oxidase; Glutathione; Humans; Hydrogen Peroxide; Iron; Magnetic Resonance Imaging; Manganese Compounds; Metal-Organic Frameworks; Mice; Neoplasms; Oxidation-Reduction; Oxides; Porphyrins; Singlet Oxygen

Nanostructures based on metal-organic frameworks (MOFs) have promising potential as theragnostic nanoplatforms for phototherapy of cancer cells. However, the MOFs alone are seldom reported to be used as photothermal agents mainly due to their poor near-infrared (NIR) light absorption.

Topics: Animals; Copper; Disease Models, Animal; Hyperthermia, Induced; Magnetic Resonance Imaging; Mice; Nanostructures; Neoplasms; Phototherapy; Porphyrins; Theranostic Nanomedicine

Numerous proteases are known to be necessary for cancer development and progression including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins. The goal of this research is to develop an Fe/Fe3O4 nanoparticle-based system for clinical diagnostics, which has the potential to measure the activity of cancer-associated proteases in biospecimens. Nanoparticle-based "light switches" for measuring protease activity consist of fluorescent cyanine dyes and porphyrins that are attached to Fe/Fe3O4 nanoparticles via consensus sequences. These consensus sequences can be cleaved in the presence of the correct protease, thus releasing a fluorescent dye from the Fe/Fe3O4 nanoparticle, resulting in highly sensitive (down to 1 × 10(-16) mol l(-1) for 12 proteases), selective, and fast nanoplatforms (required time: 60 min).

Topics: Calibration; Carbocyanines; Consensus Sequence; Enzyme Assays; Fluorescence Resonance Energy Transfer; Magnetite Nanoparticles; Matrix Metalloproteinase 13; Nanotechnology; Neoplasms; Peptide Hydrolases; Porphyrins; Reproducibility of Results; Spectrometry, Fluorescence; Surface Properties

The interaction of a series of tumor localizing porphyrins with collagen and elastin was determined. All the porphyrins studied bound to both acid soluble and insoluble collagen, but to differing extents, as well as to elastin. The extent of binding to acid soluble collagen was greater than that to insoluble collagen. The major consequence of this association was a marked decrease in the solubility of the acid soluble collagen suggesting stabilization of the fibrillar structure of the collagen had occurred. Evidence was also obtained indicating the porphyrins could bind to collagen alpha-chains. The relevance of these findings to porphyrin localization is discussed.

Topics: Chemical Phenomena; Chemistry; Collagen; Elastin; Humans; Neoplasms; Porphyrins; Protein Binding; Solubility