tellurium and Lung-Neoplasms

Topics: Cadmium Compounds; Humans; Limit of Detection; Lung Neoplasms; Mucin-1; Neoplastic Cells, Circulating; Quantum Dots; Spectrometry, Fluorescence; Tellurium

Detection of micrometastases plays an important role in early-stage and recurrent cancer diagnosis. In the study, a new method of screening micrometastases of lung cancer in peripheral blood by magnetic nanoparticles (MNPs) and quantum dots (QDs) was developed to achieve early diagnosis and recurrence prevention. MNPs were prepared by combining miniemulsion polymerization and Stöber coating methods. QDs were prepared by using Cd(Ac)(2) · 2H(2)O and oxygen-free NaHTe with thioglycolic acid as the stabilizer. The carbodiimide-mediated condensation method was used to couple pan-cytokeratin (pan-ck) antibody (Ab) to the surface of the MNPs, and Lunx and SP-A Abs to the surface of the QDs. After four kinds of epithelial tumor cells were enriched by MNPs coupled with pan-ck Ab (MNP-pan-ck), lung cancer cells A549 and SPC-A-1 were successfully identified by QDs with double-labeled Abs. Finally, 32 patients with non-small cell lung cancer (NSCLC) were collected, out of 26 cases with the enriched circulating tumor cells (CTCs), 21 cases were successfully identified by QDs. Therefore, a new method was established in which MNP-pan-ck collected CTCs and QDs with double-labeled Abs could be used simultaneously to identify CTCs from NSCLC patients.

Topics: Aged; Cadmium Compounds; Carcinoma, Non-Small-Cell Lung; Case-Control Studies; Cell Line, Tumor; Female; Glycoproteins; Hep G2 Cells; Humans; Immunohistochemistry; Keratins; Leukocytes, Mononuclear; Lung Neoplasms; Magnetite Nanoparticles; Male; Middle Aged; Neoplasm Micrometastasis; Neoplastic Cells, Circulating; Phosphoproteins; Pulmonary Surfactant-Associated Protein A; Quantum Dots; Tellurium

Selenium is an essential trace element incorporated as selenocysteine in 25 human selenoproteins. Among them are thioredoxin reductases (TrxR) and glutathione peroxidases, all central proteins in the regulation of the cellular thiol redox state. In this paper the effects of selenite and tellurite treatment in human cancer cells are reported and compared. Our results show that both selenite and tellurite, at relatively low concentrations, are able to increase the expression of mitochondrial and cytosolic TrxR in cisplatin-sensitive (2008) and -resistant (C13*) phenotypes. We further investigated the cellular effects induced by selenite or tellurite in combination with the specific TrxR inhibitor auranofin. Selenite pretreatment induced a dramatic increase in auranofin cytotoxicity in both resistant and sensitive cells. Investigation of TrxR activity and expression levels as well as the cellular redox state demonstrated the involvement of TrxR inhibition and redox changes in selenite and auranofin combined action.

Topics: Adenocarcinoma; Auranofin; Cell Death; Cell Line, Tumor; Cisplatin; Drug Resistance, Neoplasm; Enzyme Inhibitors; Female; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; Ovarian Neoplasms; Sodium Selenite; Tellurium; Thioredoxin-Disulfide Reductase

The synthesis of stable, low toxic, multifunctional, and water-soluble quantum dots (QDs) is of crucial importance for nanobiotechnology. An in situ anionic ring-opening polymerization strategy was successfully employed to grow multihydroxyl hyperbranched polyglycerol (HPG) from surfaces of aqueous synthesized QDs directly, affording multifunctional CdTe@HPG nanohybrids. The grafted HPG content can be adjusted from about 25 to 80 wt % by manipulating the feed ratio of glycidol monomer to QDs. The resultant CdTe@HPGs still show strong fluorescence and well water-solubility, and can conjugate functional biomolecules (e.g., amino acids) with their multiple reactive hydroxyls. Cytotoxicity measurements reveal that the CdTe@HPGs are much less toxic than the pristine QDs in human lung cancer cells SPCAI and more grafted HPG leads to less toxicity, due to the envelope of biocompatible HPG on QDs. It was found that the pristine QDs were unstable and their fluorescence decreased greatly or was even completed quenched after 24 h in SPCAI cells, whereas the QD@HPGs still exhibited strong fluorescence. This report opens the door for using in situ controlled/living polymerization to tailor QDs with biocompatible dendritic polymers readily and casts a light for obtaining robust nontoxic functionalized QDs and applying them in vitro and in vivo.

Topics: Biocompatible Materials; Cadmium Compounds; Cell Line, Tumor; Dendrimers; Drug Stability; Fluorescence; Glycerol; Humans; Lung Neoplasms; Polymerization; Polymers; Quantum Dots; Tellurium

New asymmetric organotellurides exhibiting good antioxidant properties in vitro and in cell culture can be attached to human serum albumin.

Topics: Antioxidants; Cell Survival; Glutathione Peroxidase; Humans; Kinetics; Lung Neoplasms; Metallothionein; Organometallic Compounds; Peroxides; Phenols; Serum Albumin; Sulfhydryl Compounds; Tellurium; Zinc

Topics: Adenocarcinoma, Papillary; Amyloidosis; Animals; Body Weight; Female; Kidney; Leukemia; Liver; Longevity; Lung; Lung Neoplasms; Lymphoma; Male; Mice; Mice, Inbred Strains; Myocardium; Osteosarcoma; Selenium; Spleen; Tellurium; Time Factors; Tooth Discoloration; Water Supply

Topics: Adult; Aged; Cysts; Diagnosis, Differential; Female; Gold Isotopes; Humans; Iodine Isotopes; Lung Diseases; Lung Neoplasms; Male; Methods; Middle Aged; Pneumonia; Pulmonary Circulation; Pulmonary Embolism; Radionuclide Imaging; Serum Albumin, Radio-Iodinated; Tellurium