technetium-tc-99m-sulfur-colloid and Carcinoma-256--Walker

Micrometastases were induced in Fisher rats using an intraportal inoculation of 0.2 ml of 8 x 10(7) Walker carcinosarcoma cells. A control group received normal saline. The hepatic perfusion index (HPI) was measured during the growth and development of micrometastases. The HPI at 4 days (0.51 +/- 0.008) and at 6 days (0.65 +/- 0.16) was significantly raised when compared to controls (0.31 +/- 0.07) and at 2 days after inoculation (0.31 +/- 0.06). Hepatic artery flow did not change throughout the study period. However, portal venous inflow was decreased significantly at 4 and 6 days (0.57 +/- 0.16 and 0.55 +/- 0.11) when compared to controls (0.96 +/- 0.34). These results indicate that the change in the hepatic perfusion index is related to a decrease in portal venous inflow. The decrease in portal venous inflow could be a mechanical effect of the micrometastases on intrahepatic blood flow or to increased arteriovenous shunting.

Topics: Animals; Carcinoma 256, Walker; Liver Circulation; Liver Neoplasms; Male; Neoplasm Transplantation; Perfusion; Radionuclide Imaging; Rats; Rats, Inbred F344; Technetium Tc 99m Sulfur Colloid

Labelled macrophages accumulate in Walker carcinosarcoma-256 after 'in vivo' and 'in vitro' stimulation with a lectin and are therefore theoretically suitable for scintigraphic tumour detection. At present, routine application of the technique in man is precluded by: the use of PHA, and a labelling method for macrophages with considerable limitations to its application and which results in significant uptake of activity in liver and spleen. However, the purpose of the study was primarily to demonstrate the principle of a possible alternative to the use of labelled monoclonal antibodies for the scintigraphic detection of tumours.

Topics: Animals; Carcinoma 256, Walker; Female; In Vitro Techniques; Monocytes; Phytohemagglutinins; Radionuclide Imaging; Rats; Rats, Inbred Strains; Technetium Tc 99m Sulfur Colloid; Time Factors