technetium-tc-99m-mebrofenin and Fatty-Liver

Topics: Aniline Compounds; Animals; Fatty Liver; Glycine; Hepatectomy; Imino Acids; Liver; Liver Function Tests; Organotechnetium Compounds; Radionuclide Imaging; Radiopharmaceuticals; Rats

Mild steatosis has been thought not to affect outcome after liver resection. However, recent studies have reported impaired postoperative recovery of patients with mild steatosis. This study evaluated the recovery of hepatic functional reserve during regeneration in a rat model of mild steatosis and liver resection.. Male Wistar rats had a standard methione- and choline-deficient diet to induce mild steatosis before 70 per cent liver resection. Evaluation of hepatobiliary function was by (99m)Tc-labelled mebrofenin scintigraphy. Mebrofenin uptake rate, the time for maximum uptake (T peak) and the time required for peak activity to decrease by 50 per cent (T(1/2) peak) were assessed 1, 2, 3 and 7 days after liver resection, along with regeneration of the remnant liver, hepatocellular and sinusoidal damage, and hepatic adenosine 5'-triphosphate (ATP) levels.. Liver regeneration and proliferative response in mild steatotic rats were no different from those in controls. However, the mebrofenin uptake rate was lower (P < 0.050) and the recovery of hepatic ATP impaired (P < 0.050) in animals with mild steatosis. Hepatocellular damage was increased (P < 0.050) but sinusoidal endothelial cell function was not affected after liver resection in mildly steatotic rats.. Mild steatosis impaired functional recovery and increased hepatocellular damage after liver resection.

Topics: Adenosine Triphosphate; Alanine Transaminase; Aniline Compounds; Animals; Aspartate Aminotransferases; Fatty Liver; Glycine; Imino Acids; Immunohistochemistry; Male; Organotechnetium Compounds; Radiopharmaceuticals; Rats; Rats, Wistar; Recovery of Function

This study evaluated the utility of non-invasive assessment of hepatobiliary function by 99mTc-mebrofenin cholescintigraphy in a rat model of diet-induced steatosis.. Male Wistar rats (250-300 g) were fed a standard methionine- and choline-deficient (MCD) diet for up to 5 weeks, thereby inducing hepatic fat accumulation, progressive inflammation and fibrogenesis corresponding with clinical steatosis. 99mTc-mebrofenin pinhole scintigraphy was used to evaluate the hepatocyte mebrofenin uptake rate, the time of maximum hepatic uptake (T(peak)) and the time required for peak activity to decrease by 50% (T(1/2peak)). Scintigraphic parameters were correlated with biochemical and serological parameters and with liver histopathology.. MCD diet induced mild steatosis after 1 week and severe steatosis with prominent inflammation after 5 weeks. T(peak), T(1/2peak) prolonged and the uptake rate decreased significantly, while the severity of steatosis increased (p<0.05). There was a strong, significant correlation between the severity of steatosis (histopathology, hepatic triglyceride content) and the 99mTc-mebrofenin uptake rate (r2=0.83, p<0.0001 and r2=0.82, p<0.0001, respectively). In addition, the uptake rate correlated significantly with the increased inflammation (plasma and hepatic TNF-alpha, r2=0.72, p<0.0001 and r2=0.52, p=0.001, respectively). The correlation of the uptake rate with hepatocellular damage was weak (AST and ALT, r2=0.29 and 0.32, respectively), but correlation with synthetic function was strong (prothrombin time, r2=0.70, p<0.001).. Hepatobiliary function assessed by 99mTc-mebrofenin scintigraphy correlates with the extent and progression of steatosis. These results suggest a potential role for mebrofenin scintigraphy as a non-invasive functional follow-up method for disease progression in steatotic patients.

Topics: Aniline Compounds; Animals; Biliary Tract; Disease Models, Animal; Fatty Liver; Glycine; Image Interpretation, Computer-Assisted; Imino Acids; Liver; Liver Function Tests; Male; Organotechnetium Compounds; Positron-Emission Tomography; Radiopharmaceuticals; Rats; Rats, Wistar; Severity of Illness Index

Topics: Aniline Compounds; Animals; Fatty Liver; Glycine; Imino Acids; Liver; Models, Biological; Organotechnetium Compounds; Positron-Emission Tomography; Radiopharmaceuticals; Rats

Topics: Acute Disease; Adult; Aniline Compounds; Fatty Liver; Female; Glycine; Hepatitis, Alcoholic; Humans; Imino Acids; Organotechnetium Compounds; Radionuclide Imaging