technetium-tc-99m-gluconate and Myocardial-Infarction

Topics: Animals; Diphosphates; Female; Gluconates; Male; Myocardial Infarction; Organotechnetium Compounds; Radionuclide Imaging; Rats; Rats, Inbred Strains; Technetium; Technetium Tc 99m Pyrophosphate; Time Factors

Topics: Adult; Aged; Animals; Dogs; Female; Gluconates; Heart; Heart Diseases; Humans; Male; Middle Aged; Myocardial Infarction; Organometallic Compounds; Organotechnetium Compounds; Radionuclide Imaging; Time Factors

99mTc-gluconate has previously been shown to bind to isolated rat heart mitochondria. In the presence of potassium cyanide this binding is enhanced several fold, an effect which has been shown to be correlated to the efficiency of the in vivo binding of the isotope complex in ischemic dog hearts. The present investigation shows that the potassium cyanide-induced binding was mainly localized to the mitochondrial inner membrane; in the presence of cyanide other organelles, e.g., nuclei and liver microsomes also showed some binding. Boiling, lipid extraction, or addition of N-ethylmaleimide caused a variable inhibition of the binding of 99mTc-gluconate to rat heart mitochondria, which also was markedly influenced by temperature, pH, time, and concentrations of protein and isotope complex, but not by conditions affecting energy levels or calcium transport. Fractionation of submitochondrial particles exposed to 99mTc-gluconate in the presence of potassium cyanide indicated that the 99mTc-gluconate-binding component cofractionated with cytochrome oxidase. It is postulated that a protein component localized in the mitochondrial inner membrane, possibly cytochrome oxidase, is responsible for the binding of 99mTc-gluconate.

Topics: Animals; Coronary Disease; Electron Transport Complex IV; Gluconates; Hydrogen-Ion Concentration; Male; Mitochondria, Heart; Myocardial Infarction; Organotechnetium Compounds; Potassium Cyanide; Radionuclide Imaging; Rats; Rats, Inbred Strains; Technetium

Topics: Adult; Aged; Diphosphates; Female; Gluconates; Heart; Humans; Male; Middle Aged; Myocardial Infarction; Organotechnetium Compounds; Radionuclide Imaging; Technetium; Technetium Tc 99m Pyrophosphate

Topics: Gluconates; Humans; Myocardial Infarction; Organotechnetium Compounds; Radionuclide Imaging; Technetium

Topics: Adult; Aged; Female; Gluconates; Heart; Humans; Male; Middle Aged; Myocardial Infarction; Organotechnetium Compounds; Radionuclide Imaging; Technetium

Binding of 99Tcm-gluconate on the subcellular level in myocardial infarct tissue in the dog was investigated by means of a fractionated centrifugation technique. High isotope activity/g protein was recorded in the mitochondrial fraction indicating the 99Tcm-gluconate binds preferentially to a protein structure localized in the mitochondria.

Topics: Animals; Cell Fractionation; Dogs; Gluconates; In Vitro Techniques; Injections, Intravenous; Mitochondria, Heart; Myocardial Infarction; Myocardium; Organotechnetium Compounds; Potassium Cyanide; Protein Binding; Rats; Technetium

Topics: Animals; Dogs; Gluconates; Myocardial Infarction; Organotechnetium Compounds; Radionuclide Imaging; Technetium; Time Factors