technetium-tc-99m-exametazime and Melanoma

Injected antigen-loaded immature monocyte-derived dendritic cells (DCs) may be incapable of migrating from skin to draining lymph nodes for antigen presentation. The in vivo migratory capacity of intradermally administered immature monocyte-derived DCs was therefore investigated during a phase I/II clinical trial for metastatic melanoma. DCs cultured from adherent monocytes in the presence of autologous serum, granulocyte-macrophage colony stimulating factor and interleukin-4 were pulsed with antigen and labelled with technetium-99m hexamethylpropylene-amineoxime (99mTc-HMPAO) ex vivo, then injected intradermally. A 99mTc-HMPAO control containing an equivalent amount of radioactivity was injected into the opposite thigh. The pelvis was then imaged with a gamma camera. The DCs were characterized as immature by functional and phenotypic analysis. Labelled DCs travelled to the draining inguinal lymph nodes within 10 min, and the draining lymph nodes were clearly outlined up to 4 h after injection. Free NmTc outlined draining lymph nodes after 10 min but was cleared from the nodes within 1 h. Thus, immature human monocyte-derived DCs migrate rapidly to and remain in draining lymph nodes after intradermal injection for immunotherapy.

Topics: Adult; Aged; Antigen Presentation; Cell Movement; Cells, Cultured; Dendritic Cells; Female; Flow Cytometry; Humans; Immunophenotyping; Immunotherapy, Adoptive; Injections, Intradermal; Lymph Nodes; Male; Melanoma; Monocytes; Pelvis; Radionuclide Imaging; Technetium Tc 99m Exametazime; Time Factors

We present an autopsy case of primary leptomeningeal melanoma visualized with technetium-99m-hexamethylpropyleneamine oxime (Tc-99m-HMPAO) single photon emission CT (SPECT) of the brain. Increased uptake of Tc-99m-HMPAO coincided with leptomeningeal enhancement on MR images and with the tumor location of the autopsy findings. It was thought that Tc-99m-HMPAO could correlate closely with melanoma. Tc-99m-HMPAO SPECT clearly showed primary leptomeningeal melanoma and severe hypoperfusion induced by intracranial hypertension and tumor proliferation.

Topics: Adult; Fatal Outcome; Humans; Male; Melanoma; Meningeal Neoplasms; Technetium Tc 99m Exametazime; Tomography, Emission-Computed, Single-Photon

A pilot study was carried out in the Nuclear Medicine Department of the National Institute of Oncology and Radiobiology to learn the diagnostic efficacy of the 99mTc-HMPAO scintigraphy in the detection of cutaneous melanoma, distant and local metastases, in patients with a confirmed histological diagnosis. The planar and/or SPECT scintigraphy was performed in 17 patients and revealed 90% sensitivity for the detection of the disease. We calculated the tumor/background index in all the images obtained and found a value greater than 1 in every case. Thus, we can affirm that the 99mTc-HMPAO accumulation in tumors was higher than the background in all cases. We recommend that this procedure be considered in the follow up of these patients as a new diagnostic method which is very useful in the determination of the patient stage and prognostic evaluation.

Topics: Adult; Aged; Aged, 80 and over; False Negative Reactions; False Positive Reactions; Female; Follow-Up Studies; Humans; Male; Melanoma; Middle Aged; Neoplasm Metastasis; Neoplasm Recurrence, Local; Pilot Projects; Radiopharmaceuticals; Sensitivity and Specificity; Skin Neoplasms; Technetium Tc 99m Exametazime; Tomography, Emission-Computed, Single-Photon

Malignant cutaneous melanoma (MCM) is a very aggressive disease that becomes in a true problem of health because of the increase in the last 5 years. In the present paper, authors report the detection of the tumor, metastases and recurrences mean 99mTc HMPAO. We presented 5 cases of MCM patients and observed the radiopharmaceutical uptake in skin, nodules and recurrent tumor lesions. Because of the number of patients was limited, authors recommended the research with a lot of patients. The gammagraphic studies could be very important for the staging of the disease and early detection of metastases and recurrent lesions.

Topics: Bone Neoplasms; Brain Neoplasms; Evaluation Studies as Topic; Female; Humans; Lymphatic Metastasis; Male; Melanoma; Middle Aged; Neoplasm Metastasis; Neoplasm Recurrence, Local; Pelvic Bones; Radionuclide Imaging; Skin Neoplasms; Technetium Tc 99m Exametazime; Technetium Tc 99m Pyrophosphate

Topics: Eye Neoplasms; Female; Humans; Male; Melanoma; Middle Aged; Radionuclide Imaging; Radiopharmaceuticals; Technetium Tc 99m Exametazime; Technetium Tc 99m Sestamibi

A number of radiopharmaceutical agents have been evaluated for the detection of malignant melanoma. Because anamine is necessary for melanin synthesis, melanotic tumors may possess numerous amine receptors. Tc-99m HMPAO consists of macrocyclic amine radicals. The purpose of this study was to evaluate Tc-99m HMPAO as an effective agent for localizing malignant melanomas on the radioisotope imaging. Six patients, with malignant melanomas were studied. Tc-99m HMPAO SPECT images were obtained in all patients. SPECT was used for the localization of the primary focus, and whole body scans were used for the evaluation of unexpected metastatic lesions. The results were compared with MRI and CT. Tc-99m HMPAO SPECT revealed a high correlation with CT and MRI for the primary focus. Abnormally increased activity at the unexpected metastatic lymph nodes in two patients were also noticed. This was confirmed by CT and MRI for those sites. Tc-99m HMPAO appears to be a useful agent for the localization of both the primary focus and unexpected metastatic lesion in patients with malignant melanoma.

Topics: Aged; Female; Humans; Magnetic Resonance Imaging; Male; Melanoma; Middle Aged; Neoplasms; Organotechnetium Compounds; Oximes; Technetium Tc 99m Exametazime; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed