technetium-tc-99m-exametazime and Cadaver

To translate spermatogonial stem cell (SSC) transplantation towards a clinical application.. Mouse green fluorescent protein (GFP)-positive testicular cells were labeled with (99m)technetium and microbubbles. These labeled cells were injected into the rete testis of isolated human testes under ultrasound guidance. Three different conditions were tested: 1) 800 μL of a 20 million cells/mL suspension; 2) 800 μL of a 10 million cells/mL suspension; and 3) 1,400 μL of a 10 million cells/mL suspension. After injection, the human cadaver testes were analyzed with the use of single-photon-emission computerized tomography (SPECT) imaging and histology.. Laboratory research environment.. Cadaver testes, obtained from autopsies at the pathology department.. Ultrasound-guided injection of mouse GFP-positive testicular cells.. Presence of radioactive-labeled cells in the human cadaver testes and GFP-positive cells in the seminiferous tubules.. In all of the experimental groups, GFP-positive cells were observed in the seminiferous tubules, near and far from the rete testis, but also in the interstitium. On SPECT, significant difference was seen between the group injected with 800 μL of a 20 million cells/mL suspension (1,654.6 ± 907.6 mm³) and the group injected with 1,400 μL of a 10 million cells/mL suspension (3,614.9 ± 723.1 mm³). No significant difference was reached in the group injected with 800 μL of a 10 million cells/mL suspension.. Injecting cells in the human cadaver testis is feasible, but further optimization is required.

Topics: Age Factors; Aged; Aged, 80 and over; Animals; Cadaver; Cell Tracking; Green Fluorescent Proteins; Humans; Injections; Male; Mice; Mice, Transgenic; Microscopy, Fluorescence; Middle Aged; Radiopharmaceuticals; Rete Testis; Seminiferous Tubules; Spermatogonia; Technetium Tc 99m Exametazime; Tomography, Emission-Computed, Single-Photon; Ultrasonography, Interventional; Young Adult

Combined pancreatic-renal transplants promise the restoration of physiologic control of serum glucose and normal renal function. As pancreatic transplantation becomes more common, there is an increased need for rapid, noninvasive evaluation of vascular graft patency and function. Pancreatic transplants share the renal transplant's complications of ischemia at harvest but are at greater risk. Tc-99m HMPAO is a lipophilic complex that clears rapidly from the blood after intravenous injection, and tissue accumulation is proportional to regional perfusion. Using Tc-99m HMPAO to monitor the vascular competency has the advantage of a high count rate during dynamic scintigrams but, in contrast to Tc-99m DTPA, has excellent delayed static images. Four patients who received combined cadaveric pancreatic-renal transplants and had a total of eight Tc-99m HMPAO scintigraphic examinations were reviewed.

Topics: Adult; Cadaver; Diabetes Mellitus, Type 1; Female; Graft Survival; Humans; Kidney Transplantation; Male; Organotechnetium Compounds; Oximes; Pancreas Transplantation; Radionuclide Imaging; Renal Insufficiency; Technetium Tc 99m Exametazime; Vascular Patency